The authors and journal apologise for an error in the above paper, which appeared in volume 13 part 12, article ID e240303 . The error relates to Fig. 5J, given on page 10, in which incorrect immunofluorescent staining images were unintentionally included in the H2O2+ML385 group panels.
The authors state that both HO-1 (Fig. 5H) and SOD2 (Fig. 5J) are indicators of antioxidant capacity and both had decreased expression in the H2O2+ML385 group in this study. Therefore, this error has no impact on the final conclusions and scientific validity of the article.
The corrected Fig. 5 is given in full below:
MT regulated the Nrf2 pathway to attenuate hepatic OS via the P62 autophagy pathway in PCOS. (A) Immunohistochemistry analysis of Keap1 in rat livers of four groups. Scale bar: 50 μm (n = 3). (B) Western blot analysis of Keap1 expression in rat livers of four groups (n = 3). (C) Western blot analysis of Beclin1, P62, LC3, and Keap1 expressions in HepG2 cells of seven groups (n = 3). (D) Immunohistochemistry analysis of Nrf2 in rat livers of four groups. Scale bar: 50 μm (n = 3). (E) Western blot analysis of Nrf2 expression in rat livers of four groups (n = 3). (F) Western blot analysis of Nrf2 expression in HepG2 cells of seven groups (n = 3). (G) Western blot analysis of HO-1, SOD1, and SOD2 expressions in HepG2 cells of seven groups (n = 3). (H–J) Immunofluorescent staining of HO-1, SOD1, and SOD2 in HepG2 cells of seven groups. Scale bar: 20 μm (n = 3). (K) Changes in the contents of MDA and antioxidant enzymes SOD, GSH-PX, and CAT in HepG2 cell supernatants of seven groups. The GSSG/GSH ratio in HepG2 cells of seven groups (n = 5). Data were presented as the mean ± SD. *P < 0.05; **P < 0.01; #P < 0.05; ##P < 0.01.
Citation: Endocrine Connections 14, 4; 10.1530/EC-24-0303e
