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María J Gómora, Flavia Morales-Vásquez, Enrique Pedernera, Delia Perez-Montiel, Horacio López-Basave, Antonio R Villa, Azucena Hernández-Martínez, Esteban Mena and Carmen Mendez

-expression of steroid receptors Receptor profile expression in each histological subtype of ovarian carcinoma has been analyzed considering the eight possible combinations of AR, ER and PR ( Fig. 3 ). HGSC was characterized by a high frequency of both triple

Open access

T P Parikh, B Stolze, Y Ozarda, J Jonklaas, K Welsh, L Masika, M Hill, A DeCherney and S J Soldin

reliability is an important advantage gained by combining information for multiple steroids. Multiplexing several steroids (profiling) offers the ability to assay all of the important analytes from a single sample. Furthermore, we used samples from healthy

Open access

Sandra R Dahl, Ingrid Nermoen, Ingeborg Brønstad, Eystein S Husebye, Kristian Løvås and Per M Thorsby

–MS/MS. The dashed lines indicate the 95% confidence interval for the regression line. (A) all samples included; (B) samples with 17OHP <100nmol/l included. Genotype, phenotype and steroid hormone profile Thirty-one individuals were classified

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Aneta Gawlik, Michael Shmoish, Michaela F Hartmann, Stefan A Wudy, Zbigniew Olczak, Katarzyna Gruszczynska and Ze’ev Hochberg

catabolized and conjugated by liver enzymes, we have anticipated that NAFLD would have its metabolic impact on steroid metabolism. Here, we utilized our previously reported concept, arguing that an individual’s urinary steroid metabolite profile represents a

Open access

Thomas Reinehr, Alexandra Kulle, Juliane Rothermel, Caroline Knop-Schmenn, Nina Lass, Christina Bosse and Paul-Martin Holterhus

, mineralocorticoids and sex hormones are a challenge because of the relatively low specificity of ELISA and RIA. Hence, the state-of-the-art method is a liquid chromatography–tandem mass spectrometry (LC–MS/MS) steroid profiling method ( 7 ), which we have used in

Open access

Thomas Reinehr, Alberto Sánchez-Guijo, Nina Lass and Stefan A Wudy

longitudinal design, the analyses of a comprehensive profile of intact steroid sulfates by state-of-the-art LC–MS/MS ( 21 ) and the study of a homogenous cohort of obese children naive to drugs. However, our study presents some potential limitations. First, BMI

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L Ghataore, I Chakraborti, S J Aylwin, K-M Schulte, D Dworakowska, P Coskeran and N F Taylor

. We report here the results of urinary steroid profiling using a high-resolution capillary column for monitoring patients after removal of an ACC who were receiving mitotane combined with hydrocortisone (cortisol). We consistently found profound

Open access

Milène Tetsi Nomigni, Sophie Ouzounian, Alice Benoit, Jacqueline Vadrot, Frédérique Tissier, Sylvie Renouf, Hervé Lefebvre, Sophie Christin-Maitre and Estelle Louiset

expression pattern of steroidogenic enzymes and determined the steroid secretion profile using cultured oncocytic cells. We could demonstrate that the oncocytic cells co-secreted cortisol and androgens, harboring therefore an intermediate steroidogenic

Open access

Paal Methlie, Steinar Hustad, Ralf Kellman, Bjørg Almås, Martina M Erichsen, Eystein S Husebye and Kristian Løvås

provides a highly sensitive, fast, comprehensive and cost-effective evaluation of patients with a range of disorders related to glucocorticoid and androgen hormones. Its utility was demonstrated by comparing the steroid profiles of women with AD with those

Open access

Willem de Ronde and Diederik L Smit

Introduction Every now and then a clinical endocrinologist will be visited by a patient that uses anabolic androgenic steroids (AAS) or has been using them in the past. The interaction between doctor and patient may be hampered for a number of