Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
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Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
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Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
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Department of Pediatrics, Copenhagen University Hospital - Herlev & Gentofte, Copenhagen, Denmark
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Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
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hospital potentially influences the hormone concentrations to be studied. Further, the collection of blood samples requires manual handling of each sample at time of the collection, centrifugation, and freezer facilities. A novel wearable system for
Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy
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handling and restraint of the animals, with a consequent activation of the hypothalamic–pituitary–adrenal (HPA) axis that quickly leads to secretion of glucocorticoids ( 7 ). For some experimental designs, faecal sampling may be a valid non
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NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark
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be affected by sampling and storage conditions, but whether these influence measured concentrations has not yet been investigated. Our study therefore seeks to define optimal conditions for handling of human plasma samples with regards to both short
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analysis using magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to investigate changes in body fat depot distribution, and a meal tolerance test (MTT) combined with arterio-venous (A-V) sampling across muscle tissue, for the first
ZLS, University of Leipzig, Leipzig, Saxony, Germany
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specimen, it becomes very important to identify degradation patterns during handling and storage and to evaluate various storage methods for samples before analysis. Here we exposed human urine samples to various short- (storage at 4°C and room
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research purposes in laboratory rodents. The replacement of radioactivity by an enzymatic label in 1971 by Engvall and Perlmann (ELISA) ( 5 ) as well as by van Weemen and Schuurs (EIA) ( 6 ), and the associated increased handling convenience led to a boost
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between methodological approaches and between visits) may be attributable to pre-analytical (e.g. specimen collection, timing of collection) and analytical error (e.g. collection techniques, sample handling). As mentioned, blood attained following
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, lasting several days after cessation of liquorice intake. We also found profoundly increased salivary cortisol after handling topical hydrocortisone close to saliva sampling and a significant increase with ≥0.5% blood contamination of the saliva
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Comprehensive Cancer Center Mainfranken, University of Wuerzburg, Wuerzburg, Germany
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Department of Pathology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Digestive and Endocrine Surgery, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Digestive and Endocrine Surgery, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Institute of Metabolism and System Research, University of Birmingham, Birmingham, UK
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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S carried out the pathology review in Cochin cohort. A J, J L, M F, S A, R L, L G, C L R, G A and J B collected the clinical data. Samples handling was performed by A J, J L and K P. A J, J L, B D, L V, A S, M N, S A, C L R and G A performed the
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inferior petrosal sinus; a careful analysis of pretest venogram is crucial, and a postsampling venography should confirm the correct positioning of the catheters. Close attention must be paid to handling of sampling. Declaration of interest The