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Jesper Krogh Department of Endocrinology & Metabolism, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark

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Peter Plomgaard Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Ruth Frikke-Schmidt Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Sten Velschow Fluisense ApS, Lillerød, Denmark

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Jesper Johannesen Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
Department of Pediatrics, Copenhagen University Hospital - Herlev & Gentofte, Copenhagen, Denmark

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Linda Maria Hilsted Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Malene Schrøder Fluisense ApS, Lillerød, Denmark

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Ulla Feldt-Rasmussen Department of Endocrinology & Metabolism, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark

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hospital potentially influences the hormone concentrations to be studied. Further, the collection of blood samples requires manual handling of each sample at time of the collection, centrifugation, and freezer facilities. A novel wearable system for

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Carlo Cinque Fondazione Ethoikos, Radicondoli, Italy
Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy

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Manuela Zinni Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy

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Anna Rita Zuena Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy

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Chiara Giuli Fondazione Ethoikos, Radicondoli, Italy

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Sebastiano G Alemà Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy

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Assia Catalani Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy

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Paola Casolini Department of Physiology and Pharmacology V. Erspamer, Sapienza University of Rome, Rome, Italy

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Roberto Cozzolino Fondazione Ethoikos, Radicondoli, Italy

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handling and restraint of the animals, with a consequent activation of the hypothalamic–pituitary–adrenal (HPA) axis that quickly leads to secretion of glucocorticoids ( 7 ). For some experimental designs, faecal sampling may be a valid non

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Nicolai J Wewer Albrechtsen NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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Monika J Bak NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark
NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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Bolette Hartmann NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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Louise Wulff Christensen NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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Rune E Kuhre NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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Carolyn F Deacon NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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Jens J Holst NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark

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be affected by sampling and storage conditions, but whether these influence measured concentrations has not yet been investigated. Our study therefore seeks to define optimal conditions for handling of human plasma samples with regards to both short

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C L Bodinham
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L Smith
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E L Thomas Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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J D Bell Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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J R Swann Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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A Costabile Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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D Russell-Jones
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A M Umpleby
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M D Robertson
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analysis using magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to investigate changes in body fat depot distribution, and a meal tolerance test (MTT) combined with arterio-venous (A-V) sampling across muscle tissue, for the first

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Franka S Schaebs Interim Group Primatology, Max Planck Institute for Evolutionary Anthropology, Leipzig, Saxony, Germany
ZLS, University of Leipzig, Leipzig, Saxony, Germany

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Gwen Wirobski Domestication Lab, Wolf Science Center, Konrad Lorenz Institute of Ethology, University of Veterinary Medicine Vienna, Vienna, Austria

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Sarah Marshall-Pescini Domestication Lab, Wolf Science Center, Konrad Lorenz Institute of Ethology, University of Veterinary Medicine Vienna, Vienna, Austria

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Friederike Range Domestication Lab, Wolf Science Center, Konrad Lorenz Institute of Ethology, University of Veterinary Medicine Vienna, Vienna, Austria

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Tobias Deschner Interim Group Primatology, Max Planck Institute for Evolutionary Anthropology, Leipzig, Saxony, Germany

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specimen, it becomes very important to identify degradation patterns during handling and storage and to evaluate various storage methods for samples before analysis. Here we exposed human urine samples to various short- (storage at 4°C and room

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Maximilian Bielohuby Sanofi-Aventis Deutschland GmbH, R&D, Industriepark Höchst, Frankfurt, Germany

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Martin Bidlingmaier Endocrine Research Laboratories, Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, Munich, Germany

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Uwe Schwahn Sanofi-Aventis Deutschland GmbH, R&D, Industriepark Höchst, Frankfurt, Germany

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research purposes in laboratory rodents. The replacement of radioactivity by an enzymatic label in 1971 by Engvall and Perlmann (ELISA) ( 5 ) as well as by van Weemen and Schuurs (EIA) ( 6 ), and the associated increased handling convenience led to a boost

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Benjamin Paul Green Department of Sport, Exercise and Rehabilitation, Faculty of Health and Life Sciences, Northumbria University, Northumberland Building, Newcastle upon Tyne NE1 8ST, UK

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Javier Thomas Gonzalez Department of Sport, Exercise and Rehabilitation, Faculty of Health and Life Sciences, Northumbria University, Northumberland Building, Newcastle upon Tyne NE1 8ST, UK

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Kevin Thomas Department of Sport, Exercise and Rehabilitation, Faculty of Health and Life Sciences, Northumbria University, Northumberland Building, Newcastle upon Tyne NE1 8ST, UK

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Emma Stevenson Department of Sport, Exercise and Rehabilitation, Faculty of Health and Life Sciences, Northumbria University, Northumberland Building, Newcastle upon Tyne NE1 8ST, UK

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Penny Louise Sheena Rumbold Department of Sport, Exercise and Rehabilitation, Faculty of Health and Life Sciences, Northumbria University, Northumberland Building, Newcastle upon Tyne NE1 8ST, UK

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between methodological approaches and between visits) may be attributable to pre-analytical (e.g. specimen collection, timing of collection) and analytical error (e.g. collection techniques, sample handling). As mentioned, blood attained following

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Marcus Imamovic Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden

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Nils Bäcklund Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden

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Staffan Lundstedt Department of Medical Biosciences, Umeå University, Umeå, Sweden

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Göran Brattsand Department of Medical Biosciences, Umeå University, Umeå, Sweden

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Elisabeth Aardal Department of Clinical Chemistry and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden

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Tommy Olsson Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden

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Per Dahlqvist Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden

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, lasting several days after cessation of liquorice intake. We also found profoundly increased salivary cortisol after handling topical hydrocortisone close to saliva sampling and a significant increase with ≥0.5% blood contamination of the saliva

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Anne Jouinot Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Juliane Lippert Department of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Wuerzburg, Wuerzburg, Germany

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Martin Fassnacht Department of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Wuerzburg, Wuerzburg, Germany
Comprehensive Cancer Center Mainfranken, University of Wuerzburg, Wuerzburg, Germany

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Bruno de La Villeon Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France

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Amandine Septier Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France

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Mario Neou Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France

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Karine Perlemoine Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France

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Silke Appenzeller Department of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Wuerzburg, Wuerzburg, Germany

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Mathilde Sibony Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Pathology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Sébastien Gaujoux Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Digestive and Endocrine Surgery, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Bertrand Dousset Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Digestive and Endocrine Surgery, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Rossella Libe Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Lionel Groussin Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Cristina L Ronchi Department of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Wuerzburg, Wuerzburg, Germany
Institute of Metabolism and System Research, University of Birmingham, Birmingham, UK
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK

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Guillaume Assié Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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Jérôme Bertherat Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France

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S carried out the pathology review in Cochin cohort. A J, J L, M F, S A, R L, L G, C L R, G A and J B collected the clinical data. Samples handling was performed by A J, J L and K P. A J, J L, B D, L V, A S, M N, S A, C L R and G A performed the

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Benedetta Zampetti Department of Endocrinology, Ospedale Niguarda, Milano, Italy

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Erika Grossrubatscher Department of Endocrinology, Ospedale Niguarda, Milano, Italy

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Paolo Dalino Ciaramella Department of Endocrinology, Ospedale Niguarda, Milano, Italy

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Edoardo Boccardi Department of Neuroradiology, Ospedale Niguarda, Milano, Italy

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Paola Loli Department of Endocrinology, Ospedale Niguarda, Milano, Italy

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inferior petrosal sinus; a careful analysis of pretest venogram is crucial, and a postsampling venography should confirm the correct positioning of the catheters. Close attention must be paid to handling of sampling. Declaration of interest The

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