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SDS–PAGE with each well loaded with 30 µg protein and transferred to nitrocellulose membranes. Primary antibodies, including CFTR (Catalog# 78335, Cell Signaling), p-CREB (Catalog# 91985, Cell Signaling) and β-tubulin (Catalog# 2146, Cell Signaling
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protein kinase A (p-PKA) and phosphorylated versions of cAMP response element-binding protein (p-CREB) were purchased from Cell Signaling Technology. The antibodies against NIS were purchased from Proteintech (Chicago, IL, USA). Reverse transcription
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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National Center of Technology Innovation for Dairy Industry, Hohhot, PR China
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. 3B and C ). Treatment with FSK and IBMX also significantly increased the transcript expression of Gja1 and Creb genes in ovine COCs compared to the control ( P < 0.05, Fig. 3D and E ). Figure 3 Effect of forskolin and IBMX on p-CX43
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relationship between cognitive impairment due to SCH and the apoptosis in cerebral cortex remains unclear. The p75 neurotrophin receptor (p75 NTR ) is an important member of the death receptor family. Although the role of the p75 NTR -mediated signal
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Endocrinology and Diabetology Unit, Diabetology and Metabolic Disease Unit, Endocrinology Unit, Unit of Human Pathology, Surgical Department, Department of Clinical Sciences and Community Health, University of Milan, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Pad.Granelli, Via F. Sforza, 35, 20122 Milan, Italy
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subunit, is required for 3T3-L1 preadipocyte differentiation . Frontiers in Bioscience 2010 2 392 – 398 . ( doi:10.2741/e99 ). 9 Delghandi MP Johannessen M Moens U . The cAMP signalling pathway activates CREB through PKA, p38 and MSK1 in NIH
Laboratory Medicine, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands
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survive because of corticoid production by adrenal-like cells in the testes, which Heikinheimo ( 20 ) and Pihlajoki ( 21 ) proposed might be similar to TART cells. Interestingly, GATA genes contain cAMP response element-binding protein (CREB) sites, and
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cAMP/PKA signaling inhibits the secretion of progesterone via PKA ( 50 ) and the transcription of beta-hCG via CREB ( 49 ). The activated EP3/G i1 can stimulate p-ERK1/2 and p53, which eventually enhance PAI-1 gene transcription ( 52 ). These changes
Department of Endocrinology and Metabolism, People's Hospital of Liaoning Province, Shenyang, People’s Republic of China
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Department of Laboratory Medical, The First Hospital of China Medical University, Shenyang, People’s Republic of China
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Institute of Health Guide for the Care and Use of Laboratory Animals. The rats were randomly assigned to three groups: control group (CON, n = 35), OH group ( n = 35) and SCH group ( n = 35). The rats were treated by intraperitoneal (i.p.) injection of 3
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analyze plasma parameters, tissue glycogen contents, related gene and protein expression levels, and hepatic enzyme activities. Tukey’s multiple test was used to compare the means at a significance level of P < 0.05. All data are presented as the mean
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has been shown to affect the signalling pathway mediated by the cAMP-dependent protein kinase (PKA) activation and cAMP-responsive element-binding protein (CREB) phosphorylation in different cell types ( 12 , 27 ). In hepatocytes, biguanides suppress