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Sandra R Dahl, Ingrid Nermoen, Ingeborg Brønstad, Eystein S Husebye, Kristian Løvås, and Per M Thorsby

for the patient’s age and sex. However, current immunoassays for testosterone are prone to interferences in the female reference range (low levels of testosterone) and can be inaccurate ( 7 , 8 , 9 ). Liquid chromatography–tandem mass spectrometry

Open access

Letícia Ribeiro Oliveira, Carlos Alberto Longui, Guilherme Guaragna-Filho, José Luiz Costa, Rafael Lanaro, David Antônio Silva, Maria Izabel Chiamolera, Maricilda Palandi de Mello, André Moreno Morcillo, Andrea Trevas Maciel-Guerra, and Gil Guerra-Junior

laboratories ( 3 , 4 ). More recently, liquid chromatography coupled with mass spectrometry in tandem (LC-MS/MS) has replaced conventional methods of steroid hormone analysis, enabling the use of small volume samples, improving specificity and reducing the

Open access

Muriel Houang, Thao Nguyen-Khoa, Thibaut Eguether, Bettina Ribault, Séverine Brabant, Michel Polak, Irène Netchine, and Antonin Lamazière

was eventually positively diagnosed thanks to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), which allowed the quantitation of endogenous steroid metabolites and the identification of exogenous glucocorticoid treatment on a

Open access

Niek F Dirks, Etienne Cavalier, and Annemieke C Heijboer

). Measurement results are therefore to be reported in ‘nmol/L’ in the case of 25(OH)D and 24,25(OH) 2 D or ‘pmol/L’ for 1,25(OH) 2 D. Measurement preanalysis For 25(OH)D, both automated assays and liquid chromatography–tandem mass spectrometry (LC-MS/MS

Open access

Paal Methlie, Steinar Hustad, Ralf Kellman, Bjørg Almås, Martina M Erichsen, Eystein S Husebye, and Kristian Løvås

factors likely to slow its adoption. The development of simple, reliable, and cost- and labour-efficient methods is, therefore, important. Liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is a highly selective mode of detection. It

Open access

Lauren R Cirrincione, Bridgit O Crews, Jane A Dickerson, Matthew D Krasowski, Jessica Rongitsch, Katherine L Imborek, Zil Goldstein, and Dina N Greene

effect of unconjugated estrone concentrations on estradiol immunoassay interference, we determined analytical bias relative to liquid chromatography tandem mass spectrometry (LC-MS/MS) in three estradiol commercial immunoassays among transgender women

Open access

M P Schuijt, C G J Sweep, R van der Steen, A J Olthaar, N M M L Stikkelbroeck, H A Ross, and A E van Herwaarden

chromatography tandem mass spectrometry (ED-LC-MS/MS). LC-MS/MS enables the accurate, specific and matrix-independent measurement of testosterone with direct calibration. Equilibrium dialysis enables to separate the free- from protein-bound testosterone with

Open access

T P Parikh, B Stolze, Y Ozarda, J Jonklaas, K Welsh, L Masika, M Hill, A DeCherney, and S J Soldin

of high-performance liquid chromatography (HPLC) in conjunction with tandem mass spectrometry (MS) is increasingly becoming the method of choice for clinical testing of hormone levels ( 5 , 6 , 15 , 16 , 17 ). Given its high sensitivity

Open access

Johanna Christina Penell, Mark M Kushnir, Lars Lind, Jonatan Bergquist, Jonas Bergquist, P Monica Lind, and Tord Naessen

concentrations seen in elderly ( 25 , 26 , 27 ). We recently developed highly sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods to identify and quantify a large number of steroids in small sample volumes ( 13 , 14 , 16

Open access

Fidéline Bonnet-Serrano, Maxime Barat, Anna Vaczlavik, Anne Jouinot, Lucas Bouys, Christelle Laguillier-Morizot, Corinne Zientek, Catherine Simonneau, Etienne Larger, Laurence Guignat, Lionel Groussin, Guillaume Assié, Jean Guibourdenche, Ioannis Nicolis, Marie-Claude Menet, and Jérôme Bertherat

nitrogen. Dried extracts were further reconstituted in 100 µL of MeOH/water (50/50). Conditions and instrumentation for ultra-high-performance liquid chromatography-mass spectrometry Steroid profiles were characterized using LC-MS/MS. Ten