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venous thromboembolism ( 6 , 7 , 8 , 9 ). Although reducing fasting plasma glucose levels to normal is seen as a way to prevent negative cardiovascular outcomes, such as myocardial infarction or stroke ( 10 ), the most recent study with insulin
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, most teleost fish are considered to be glucose-intolerant with a persistent hyperglycemia often observed after a glucose load or the ingestion of carbohydrate-rich diets ( 1 , 2 , 3 ). At present, several valid hypotheses have been proposed to explain
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). Briefly, after fasting for 6 h, mice were intraperitoneally injected with glucose at a dose of 2 g/kg body weight. Blood glucose levels were measured at the indicated time points after glucose injection using a glucometer (Roche Diagnostics). For the
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( 17 ), or 1 mg/kg atosiban ( 17 ), or saline (0.9% NaCl, used as a vehicle) 30 min before glucose loading (Six groups, n = 8). The GTT was then initiated by an intraperitoneal injection of 2 g/kg glucose, as previously described ( 18 ). Briefly, the
Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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and HMW adiponectin determination. Oral glucose tolerance test (OGTT) OGTT was performed after 10 weeks of diet and exercise intervention. Following overnight fasting, mice were given a glucose load (1 mg/g) orally. Blood sample was collected
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) GTT and ITT were performed on GD 15. For GTT, mice, after 6 h fasting, received intraperitoneal (i.p.) injections of glucose at 2 g per kg body weight. Blood samples at 0, 30 and 60 min following glucose administration were obtained from the tail via
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mating was 70%, and the mean success of pregnancy in the second mating was 74%. Intraperitoneal glucose (ipGTT) and insulin tolerance test (ipITT) Animals were submitted to overnight fasting and blood glucose was measured (time 0). For ipGTT, a
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BMI, that were higher in the HSD groups compared to their controls. In addition all the aforementioned parameters were different between males and females. Insulin resistance We performed an intraperitoneal glucose tolerance test (IPGTT, Fig
Department of Anatomy, Shanxi Medical University, Taiyuan, China
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min after administration. For the insulin tolerance test (ITT), mice were fasted for 6 h. The fasting blood glucose was measured, and then, the mice were injected intraperitoneally with 0.075 U/mL human insulin in filter-sterilized PBS at 0.1 mL per 10
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healthcare research ( 2 , 3 ). For obesity prevention, most of the interest in developing alternative strategies targeting related processes, namely glucose transport and intake, differentiation of adipogenesis, suppression of inflammation and changes in