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Stefan Schulz, Anika Mann, Benjamin Novakhov, Hugh D Piggins and Amelie Lupp

receptor or VPAC2 receptor. Stably transfected cells were grown in medium supplemented with 1 μg/ml gentamicin. Immunocytochemistry Stably transfected HEK 293 cells were grown on poly- l -lysine-coated coverslips overnight, fixed with 4% paraformaldehyde

Open access

Simon Schimmack, Yongchao Yang, Klaus Felix, Markus Herbst, Yixiong Li, Miriam Schenk, Frank Bergmann, Thilo Hackert and Oliver Strobel

imaging, Damme, Germany). Immunohistochemistry and immunocytochemistry The expression of CRP and IL-6 was assessed on paraffin-embedded primary tumor samples by immunohistochemistry and on BON1 cells by immunocytochemistry as previously described

Open access

Earn H Gan, Wendy Robson, Peter Murphy, Robert Pickard, Simon Pearce and Rachel Oldershaw

/75 cm 2 density. All cell culture reagents were purchased from Gibco (Invitrogen) unless otherwise stated. Immunocytochemistry/immunofluorescent staining study Following first passage of the primary cell culture, cells were re-plated onto

Open access

Linfei Yang, Xiao Yu and Yongchao Yang

Although the upregulation of autotaxin (ATX) is associated with many solid tumours, its role in pancreatic neuroendocrine neoplasms (pNEN) has not been well elucidated. The expression of ATX in pNEN tissues and pNEN cell line BON1 was analysed by Western blot, PCR and immunocytochemistry upon exposure to interleukin-6 (IL-6). Additionally, pNEN cell line BON1 was transfected with siRNAs against ATX or signal transducer and activator of transcription 3 (STAT3) and assessed by in vitro invasion assays. The following results were obtained. The expression of ATX in pNEN tissues was significantly increased compared with that in normal pancreatic tissues. High ATX expression was strongly correlated with tumour grade, lymph node metastasis and tumour-node-metastasis stage. Furthermore, ATX downregulation notably inhibited the metastatic capacity of pNEN cells, whereas STAT3 knockdown was found to downregulate the expression of ATX. ATX expression was upregulated in BON1 cells upon stimulation with IL-6, and this was accompanied by activation/phosphorylation of STAT3. Western blot analysis of human pNEN tissue extracts confirmed increased ATX expression and STAT3 phosphorylation with elevated expression levels of IL-6. In conclusion, ATX is upregulated in pNEN and is correlated with the metastatic capacity of pNEN cells, potentially via interaction with STAT3 activation.

Open access

Melissa Braga, Zena Simmons, Keith C Norris, Monica G Ferrini and Jorge N Artaza

-life of 1,25-D 3 , the cell culture media, incubated with or without 1,25-D3 (100 nM), was replaced daily ( 11 , 12 ). Detection of PAX7, VDR and MYC by immunocytochemistry Satellite cells incubated in growth media for 1 day in collagen

Open access

Michael J Gratz, Stavroula Stavrou, Christina Kuhn, Simone Hofmann, Kerstin Hermelink, Helene Heidegger, Stefan Hutter, Doris Mayr, Sven Mahner, Udo Jeschke and Aurelia Vattai

, Hopkinton, MA, USA) for 2 h (PCR samples) and 48 h (immunocytochemistry and Western blot samples), respectively. Control cells were incubated sans stimulants. Table 4 Raw numbers to Fig. 2. IRS mean IRS 25th percentile IRS 75th

Open access

Tsuneo Ogawa and Adolfo J de Bold

association of ANF with the atrial-specific granules was demonstrated by both tissue fractionation studies and by immunocytochemistry (14, 15) . The presence of natriuretic activity in the atria of mammals and in both the atria and ventricles of non

Open access

Stavroula Stavrou, Michael Gratz, Eileen Tremmel, Christina Kuhn, Simone Hofmann, Helene Heidegger, Mina Peryanova, Kerstin Hermelink, Stefan Hutter, Bettina Toth, Doris Mayr, Sven Mahner, Udo Jeschke and Aurelia Vattai

: BCBL2185V, Buchs, Switzerland), 3-Iodothyronamine (T 1 AM) (Cayman Chemical) and RO5203548 (Glixx Laboratories, Hopkinton, MA, USA) for 2 h (PCR samples) and 48 h (Immunocytochemistry and Western blot samples), respectively. Control cells were incubated

Open access

Yosep Chong, Soon-Jin Ji, Chang Suk Kang and Eun Jung Lee

7 – 13 . ( doi:10.1002/dc.10391 ) 10.1002/dc.10391 14696138 9 Fadda G Rossi ED Mulè A Miraglia A Vecchio FM Capelli A. Diagnostic efficacy of immunocytochemistry on fine needle aspiration biopsies processed by thin