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, and aggressiveness e.g., non-progressive vs malignant/metastatic) ( 41 , 42 ) and have demonstrable predictive utility at a tissue level ( 43 ). More recently, blood-based assays (CTCs, miRNA and circulating mRNA) have been developed. The most

in clinical samples and experimental systems and thereby to sensitising tumour epithelial cells to low androgen levels ( 23 , 24 ). Detection of AR amplification in circulating tumour cells (CTCs) and circulating tumour DNA (ctDNA) isolated from

′→3′) Temp. (°C) Amp. size (bp) GAPDH FwdRev TGA ACG GGA AGC TCA CTG GTCC ACC ACC CTG TTG CTG TA 60 306 IL-2 FwdRev CTG ACG CTT GTC CTC CTT GTCTGC TGC TGT GTT TCC TTT GC 60 64 IL-10 FwdRev CAT GGC CCA GAA ATC AAG

polymorphism was conducted using restriction enzyme PvuII following gel agarose electrophoresis. Study of rs53576(G→A) polymorphism of oxytocin receptor gene ( OXTR ) was conducted using PCR method. The primers were F: 5′-GCC CAC CAT GCT CTC CAC ATC-3′ και R

. Gene Primer sequences HPRT ( hprt ) Forward 5′-GCA GTA CAG CCC CAA AAT GG-3′ Reverse 5′-AAC AAA GTC TGG CCT GTA TCC AA-3′ Ubiquitin ( Ubc ) Forward 5′-AGC CCA GTG TTA CCA CCA AG-3′ Reverse 5′-CTA AGA CAC CTC CCC CAT CA-3′ EF1

GTT GT REN L43524 GAC CGA GGA CGT CTT CTC C 378 62 CTT GGA GGC AAA GCC TAC AC ACE TC255148 TCT CGC TGC TGA GAA GAT CA 359 60 CAT TCG GGT CAG GAA GTT GT AGTR1 NM_174233 CAT TAC GAA TCC CAA AAT TCT ACC 344 60 AGG CAA TTG TTA AAA TAA GCC AAG AGTR2 NM

, 5′-GGG GCG GGT GGA ACC TTA G-3′; 2F, 5′-TTG GGT CAC AGG CTT GGA AAG-3′; 3R, ACA GGG ACC ACC CAC CAT GTG-3′; 8F, 5′-AAG AAT GTT CCC AAC CTA TGC-3′; 9R, 5′-GCA GAA CAT GGG CTC AGA GTT G-3′. Next-generation sequencing (NGS) NGS was performed in

interventions: the TREND statement . American Public Health Association 2004 94 361 – 366 . ( https://doi.org/10.2105/AJPH.94.3.361 ) 10.2105/AJPH.94.3.361 11 Common Terminology Criteria for Adverse Events (CTCAE) Version 4.0 . (available from: http://ctep.cancer.gov/reporting/ctc

′ Reverse 5′ TGA CAA GCC ACA GAA CTC TC 3′ β-Tsh Forward 5′ TCT GCG CTG GGT ATT GTA TG 3′ Reverse 5′ CGG TAT TTC CAC CGT TCT GT 3′ Gapdh Forward 5′ TGA TTC TAC CCA CGG GAA GT 3′ Reverse 5′ AGC ATC ACC CCA TTT GAT GT

reported. Funding This research was funded by the Ingham Institute for Applied Medical Research CTC Head & Neck Research Grant. T L R is the recipient of a Cancer Institute New South Wales Future Research Leader Fellowship. M J A is the recipient of