Department of Endocrinology, Zunyi Medical University, Zunyi, China
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targets, and sample-specific barcode sequences were used for PCR amplification of the variable regions of the 16S rRNA gene (Q5 HiFi DNA polymerase; NEB, Ipswich, MA, USA). The amplification products were subjected to 2% agarose gel electrophoresis, and
Faculty of Medicine, University of Freiburg, Freiburg, Germany
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Faculty of Medicine, University of Freiburg, Freiburg, Germany
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Faculty of Medicine, University of Freiburg, Freiburg, Germany
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Faculty of Medicine, University of Freiburg, Freiburg, Germany
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Faculty of Medicine, University of Freiburg, Freiburg, Germany
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Faculty of Medicine, University of Freiburg, Freiburg, Germany
Institute of Veterinary Medicine, Georg-August-University Goettingen, Goettingen, Germany
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Cancer Research 2014 16 496. ( https://doi.org/10.1186/s13058-014-0496-5 ) 34 Kokolo M Bach-Elias M Downregulation of p68 RNA helicase (DDX5) activates a survival pathway involving mTOR and MDM2 signals . Folia Biologica 2017 63 52 – 59
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(Epicenter, Madison, WI, USA) and further treated with RNase R (Epicenter). The total RNA from each tissue specimen was isolated by RNA extraction using TRIzol (Invitrogen) according to the manufacturer’s recommendations. The concentration and purity of RNA
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continuing with the extraction. Average RNA yield was 4725 ng ( s.e.m. ± 319.7) and 4942 ng ( s.e.m. ± 368.7) for RCD and HFD (respectively). RNA (1–2 µg) was reverse transcribed into cDNA using the QuantiTect RT Kit (205314; Qiagen) in the MJ Research PTC
Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Guangdong Geriatric Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China
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Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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Reproductive Medicine Center, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China
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Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China
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Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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.1007/978-1-4939-8982-9_1 ) 37 Xu J Cao X Long noncoding RNAs in the metabolic control of inflammation and immune disorders . Cellular and Molecular Immunology 2019 16 1 – 5 . ( https://doi.org/10.1038/s41423-018-0042-y ) 38 Dhodapkar KM Friedlander D Scholes
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Center of Innovation in Personalized Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
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represents an essential resource for genetic studies because of the consanguinity rate of >50%, the high fertility levels, and the territory’s geography that favor the genetic isolation of this region ( 16 ). In fact, the high incidence of homozygous
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://doi.org/10.1093/bib/bbw042 ) 5 Yang Y Liu M Yang F Wang X Bai X Mu S Liu Y Hu D . Circular RNA expression profiles following negative pressure wound therapy in burn wounds with experimental Pseudomonas aeruginosa infection . Bioengineered
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. Manual micro-dissection was performed to maximize the amount of tumor cells. DNA was extracted and purified by using the QIAamp DNA Mini Kit (Qiagen), according to the manufacturer’s protocol. DNA was eluted in 50 μL of elution buffer. RNA was isolated
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tissues was extracted from frozen adrenal glands using Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. Adrenal glands from two to three animals were pooled as one biological replicate
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Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK
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samples using TRIzol reagent (Invitrogen) as per the manufacturer’s instructions. cDNA was then synthesised from total RNA using the QuantiTect reverse transcriptase kit (Qiagen) as per the manufacturer’s instructions. Expression levels of mRNA in adult