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Open access

Aneta Gawlik, Michael Shmoish, Michaela F Hartmann, Stefan A Wudy, Zbigniew Olczak, Katarzyna Gruszczynska and Ze’ev Hochberg

Objective

Analysis of steroids by gas chromatography-mass spectrometry (GC-MS) defines a subject’s steroidal fingerprint. Here, we compare the steroidal fingerprints of obese children with or without liver disease to identify the ‘steroid metabolomic signature’ of childhood nonalcoholic fatty liver disease.

Methods

Urinary samples of 85 children aged 8.5–18.0 years with BMI >97% were quantified for 31 steroid metabolites by GC-MS. The fingerprints of 21 children with liver disease (L1) as assessed by sonographic steatosis (L1L), elevated alanine aminotransferases (L1A) or both (L1AL), were compared to 64 children without markers of liver disease (L0). The steroidal signature of the liver disease was generated as the difference in profiles of L1 against L0 groups.

Results

L1 comparing to L0 presented higher fasting triglycerides (P = 0.004), insulin (P = 0.002), INS/GLU (P = 0.003), HOMA-IR (P = 0.002), GGTP (P = 0.006), AST/SGOT (P = 0.002), postprandial glucose (P = 0.001) and insulin (P = 0.011). L1AL showed highest level of T-cholesterol and triglycerides (P = 0.029; P = 0.044). Fasting insulin, postprandial glucose, INS/GLU and HOMA-IR were highest in L1L and L1AL (P = 0.001; P = 0.017; P = 0.001; P = 0.001). The liver disease steroidal signature was marked by lower DHEA and its metabolites, higher glucocorticoids (mostly tetrahydrocortisone) and lower mineralocorticoid metabolites than L0. L1 patients showed higher 5α-reductase and 21-hydroxylase activity (the highest in L1A and L1AL) and lower activity of 11βHSD1 than L0 (P = 0.041, P = 0.009, P = 0.019).

Conclusions

The ‘steroid metabolomic signature’ of liver disease in childhood obesity provides a new approach to the diagnosis and further understanding of its metabolic consequences. It reflects the derangements of steroid metabolism in NAFLD that includes enhanced glucocorticoids and deranged androgens and mineralocorticoids.

Open access

T P Parikh, B Stolze, Y Ozarda, J Jonklaas, K Welsh, L Masika, M Hill, A DeCherney and S J Soldin

reliability is an important advantage gained by combining information for multiple steroids. Multiplexing several steroids (profiling) offers the ability to assay all of the important analytes from a single sample. Furthermore, we used samples from healthy

Open access

Sandra R Dahl, Ingrid Nermoen, Ingeborg Brønstad, Eystein S Husebye, Kristian Løvås and Per M Thorsby

advantage of LC–MS/MS is that several hormones and glucocorticoid medication can be measured in one sample/run and an adrenal steroid profile can be established. By multiplexing, valuable information is gained. Furthermore, the updated Clinical Practice

Open access

L Ghataore, I Chakraborti, S J Aylwin, K-M Schulte, D Dworakowska, P Coskeran and N F Taylor

. We report here the results of urinary steroid profiling using a high-resolution capillary column for monitoring patients after removal of an ACC who were receiving mitotane combined with hydrocortisone (cortisol). We consistently found profound

Open access

Paal Methlie, Steinar Hustad, Ralf Kellman, Bjørg Almås, Martina M Erichsen, Eystein S Husebye and Kristian Løvås

provides a highly sensitive, fast, comprehensive and cost-effective evaluation of patients with a range of disorders related to glucocorticoid and androgen hormones. Its utility was demonstrated by comparing the steroid profiles of women with AD with those

Open access

Thomas Reinehr, Alexandra Kulle, Juliane Rothermel, Caroline Knop-Schmenn, Nina Lass, Christina Bosse and Paul-Martin Holterhus

, mineralocorticoids and sex hormones are a challenge because of the relatively low specificity of ELISA and RIA. Hence, the state-of-the-art method is a liquid chromatography–tandem mass spectrometry (LC–MS/MS) steroid profiling method ( 7 ), which we have used in

Open access

Bruno Donadille, Muriel Houang, Irène Netchine, Jean-Pierre Siffroi and Sophie Christin-Maitre

treatment was administered to the patient. LC–MS/MS steroids profile analysis showed that 11betahydroxy-androstenedione (11OHA4) plasma levels were below the detection limit ( N : 3–10 nmol/L). Table 1 Hormonal status at age 22 years

Open access

Annette Mouritsen, Alexander Siegfried Busch, Lise Aksglaede, Ewa Rajpert-De Meyts and Anders Juul

435 – e445 . ( https://doi.org/10.1111/j.1365-2605.2011.01173.x ) 10.1111/j.1365-2605.2011.01173.x) 21 Strahm E Sottas PE Schweizer C Saugy M Dvorak J Saudan C. Steroid profiles of

Open access

Eric Seidel, Gudrun Walenda, Clemens Messerschmidt, Benedikt Obermayer, Mirko Peitzsch, Paal Wallace, Rohini Bahethi, Taekyeong Yoo, Murim Choi, Petra Schrade, Sebastian Bachmann, Gerhard Liebisch, Graeme Eisenhofer, Dieter Beule and Ute I Scholl

. Mitotane-resistant cells show impaired production of adrenal steroid hormones Next, we measured levels of adrenal steroid hormones in cell supernatants of founder cells, nonresistant and resistant cells by LCMS ( Fig. 6 ). Steroid profiles of the founder

Open access

Jonneke J Hollanders, Bibian van der Voorn, Noera Kieviet, Koert M Dolman, Yolanda B de Rijke, Erica L T van den Akker, Joost Rotteveel, Adriaan Honig and Martijn J J Finken

utero . Acta Neuropsychiatrica 2017 29 43 – 53 . ( doi:10.1017/neu.2016.30 ) 10.1017/neu.2016.30 27387606 20 Noppe G de Rijke YB Dorst K van den Akker EL van Rossum EF. LC–MS/MS-based method for long-term steroid profiling