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Amarjit Saini, Linda Björkhem-Bergman, Johan Boström, Mats Lilja, Michael Melin, Karl Olsson, Lena Ekström, Peter Bergman, Mikael Altun, Eric Rullman and Thomas Gustafsson

satellite cell (SC) niche, i.e. myoblast stem cells ( 22 ). Adult skeletal muscle is terminally differentiated and in response to injury, a local pool of SCs situated beneath the basal lamina surrounding each myofibre proliferate ( 23 ). Their progeny

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Melissa Braga, Zena Simmons, Keith C Norris, Monica G Ferrini and Jorge N Artaza

C12 cells ( 11 , 12 , 13 , 14 ). Mouse C2C12 skeletal muscle myoblast cells are an ‘ in vitro ’ cell line, which is widely used to study genes that regulate muscle growth and differentiation ( 15 , 16 ). Satellite cells, also known as ‘skeletal

Open access

Flavia Letícia Martins Peçanha, Reinaldo Sousa dos Santos and Wagner Seixas da-Silva

myoblast cell line was obtained from BCRJ (Banco de Células do Rio de Janeiro) and certified to be free from mycoplasma contamination. The cells were grown in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 nmol/L sodium selenite

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Selina Mäkinen, Yen H Nguyen, Paulina Skrobuk and Heikki A Koistinen

bovine serum (FBS), 1% penicillin, 1% streptomycin and 1% fungizone. Primary myoblasts were separated from non-myogenic cells with CD56-coupled magnetic beads (Miltenyi Biotec, Gologne, Germany). To obtain myotubes, myoblasts at 80% confluence were

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Fernanda A Correa, Ericka B Trarbach, Cintia Tusset, Ana Claudia Latronico, Luciana R Montenegro, Luciani R Carvalho, Marcela M Franca, Aline P Otto, Everlayny F Costalonga, Vinicius N Brito, Ana Paula Abreu, Mirian Y Nishi, Alexander A L Jorge, Ivo J P Arnhold, Yisrael Sidis, Nelly Pitteloud and Berenice B Mendonca

of FGFR1 mutants was assessed using L6 myoblast cells as described previously (15) . In brief, cells were transiently transfected with WT or altered FGFR1c expression vector in combination with the osteocalcin FGF response element luciferase reporter

Open access

Miranda Scharff, Chantal Maria Wiepjes, Maartje Klaver, Thomas Schreiner, Guy T’Sjoen and Martin den Heijer

effect on myoblast proliferation and myoblast differentiation, and testosterone increases the number of satellite cells, which promotes protein synthesis of muscle mass ( 16 ). Thus, testosterone plays an important role in muscle mass and muscle strength

Open access

Joanna Klubo-Gwiezdzinska, John Costello Jr, Kirk Jensen, Aneeta Patel, Rok Tkavc, Douglas Van Nostrand, Kenneth D Burman, Leonard Wartofsky and Vasyl Vasko

coworkers documented synergistic effects of amifostine and radiation in induction of apoptosis of myoblast cells ( 24 ). Dai and coworkers revealed dual-specificity of amifostine action: anticancer synergy between amifostine and paclitaxel in vitro and in

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Anne H van der Spek, Olga V Surovtseva, Saskia Aan, Anton T J Tool, Annemarie van de Geer, Korcan Demir, Anja L M van Gucht, A S Paul van Trotsenburg, Timo K van den Berg, Eric Fliers and Anita Boelen

7 Milanesi A Lee JW Kim NH Liu YY Yang A Sedrakyan S Kahng A Cervantes V Tripuraneni N Cheng SY Thyroid hormone receptor alpha plays an essential role in male skeletal muscle myoblast proliferation

Open access

M L M Barreto-Chaves, N Senger, M R Fevereiro, A C Parletta and A P C Takano

thyroid hormones on the Na/H antiport in L-6 myoblasts: high molecular specificity for 3,3,5-triiodo-l-thyronine . Endocrinology 1999 140 683 – 689 . ( ) 40 Cody V Davis PJ Davis FB . Molecular