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Simonetta Piana, Eleonora Zanetti, Alessandra Bisagni, Alessia Ciarrocchi, Davide Giordano, Federica Torricelli, Teresa Rossi and Moira Ragazzi

analyzing by immunohistochemistry the expression of NOTCH1 in a large series of thyroid neoplasms with distinct histogenesis and very different biological behavior. Materials and methods Case selection The study was conducted by the Pathology

Open access

Catarina Tavares, Maria João Coelho, Catarina Eloy, Miguel Melo, Adriana Gaspar da Rocha, Ana Pestana, Rui Batista, Luciana Bueno Ferreira, Elisabete Rios, Samia Selmi-Ruby, Bruno Cavadas, Luísa Pereira, Manuel Sobrinho Simões and Paula Soares

). These observations suggest that in thyroid carcinomas, besides transcription regulation, NIS expression appears to be modulated by post-transcriptional events. Therefore, studies of NIS expression by immunohistochemistry (IHC) ( 1 , 9 , 10 , 11 , 12

Open access

Stefan Schulz, Anika Mann, Benjamin Novakhov, Hugh D Piggins and Amelie Lupp

of great advantage to know the receptor expression of the tumour. However, the widespread application of immunohistochemistry for in vitro VIP receptor evaluation has been hampered by the lack of MABs and the limited availability of specific

Open access

Sara Storvall, Helena Leijon, Eeva Ryhänen, Johanna Louhimo, Caj Haglund, Camilla Schalin-Jäntti and Johanna Arola

-JT), characterized by early-onset PHPT, ossifying jaw fibromas, kidney and uterine tumors, but may also cause familial isolated hyperparathyroidism ( 15 , 16 , 17 ). Parafibromin immunohistochemistry is thus a useful marker. The specificity of negative staining is

Open access

Tao Mei, Jianhe Zhang, Liangfeng Wei, Xingfeng Qi, Yiming Ma, Xianhua Liu, Shaohua Chen, Songyuan Li, Jianwu Wu and Shousen Wang

Plasma membrane Polyclonal/rabbit Placenta HIF-1α Nucleus and cytoplasm Polyclonal/mouse Human gastric cancer tissue EliVision immunohistochemistry was performed using a two-step method, according to the manufacturer

Open access

Tiina Vesterinen, Teijo Kuopio, Maarit Ahtiainen, Aija Knuuttila, Harri Mustonen, Kaisa Salmenkivi, Johanna Arola and Caj Haglund

cores were marked. The TMAs were constructed using a TMA Grand Master (3DHISTECH, Budapest, Hungary) or Galileo TMA CK4500 microarrayer (Isenet, Milan, Italy). Immunohistochemistry Fresh 3.5 µm thick TMA sections were cut with a microtome onto

Open access

Adrian F Daly, David A Cano, Eva Venegas-Moreno, Patrick Petrossians, Elena Dios, Emilie Castermans, Alvaro Flores-Martínez, Vincent Bours, Albert Beckers and Alfonso Soto-Moreno

approved by the Ethics Committees of the CHU de Liège and the Hospital Universitario Virgen del Rocío. Immunohistochemistry for AIP We undertook a specific study of immunohistochemistry for AIP (AIP-IHC) in a series of 51 somatotropinomas operated

Open access

María J Gómora, Flavia Morales-Vásquez, Enrique Pedernera, Delia Perez-Montiel, Horacio López-Basave, Antonio R Villa, Azucena Hernández-Martínez, Esteban Mena and Carmen Mendez

and could help characterize ovarian carcinomas. Materials and methods In this retrospective study, we evaluated the expression of AR, ER, PR and the cell proliferation biomarker Ki-67 by immunohistochemistry. We used 81 samples of ovarian

Open access

Manon Engels, Paul N Span, Rod T Mitchell, Joop J T M Heuvel, Monica A Marijnissen-van Zanten, Antonius E van Herwaarden, Christina A Hulsbergen-van de Kaa, Egbert Oosterwijk, Nike M Stikkelbroeck, Lee B Smith, Fred C G J Sweep and Hedi L Claahsen-van der Grinten

right testis) were previously collected as described by Claahsen and coworkers ( 3 , 5 ) (informed consent was obtained). Paraffin-embedded material for immunohistochemistry was available for all tumours, while frozen material for RNA isolation was

Open access

Wei Li, Qing Huang, Danyang Sun, Guizhi Zhang and Jian Tan

The specimens were immunostained using the PV-6000 immunohistochemistry method to evaluate the expression of the RDM1 protein. The primary antibody used was RDM1 rabbit polyclonal antibody (HPA024708, Sigma). The samples stained with RDM1