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Ayako Sato Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Katsuya Matsuda Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Takahiro Motoyama Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Zhanna Mussazhanova Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Al-Farabi Kazakh National University, Almaty City, Republic of Kazakhstan

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Ryota Otsubo Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Hisayoshi Kondo Biostatics Section, Division of Scientific Data Registry, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Yuko Akazawa Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Miyoko Higuchi Department of Diagnostic Pathology and Cytology, Kuma Hospital, Kobe, Hyogo, Japan

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Ayana Suzuki Department of Diagnostic Pathology and Cytology, Kuma Hospital, Kobe, Hyogo, Japan

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Mitsuyoshi Hirokawa Department of Diagnostic Pathology and Cytology, Kuma Hospital, Kobe, Hyogo, Japan

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Akira Miyauchi Department of Diagnostic Pathology and Cytology, Kuma Hospital, Kobe, Hyogo, Japan

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Takeshi Nagayasu Department of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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Masahiro Nakashima Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

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together with other DDR molecules, such as γH2AX and ATM ( 4 , 5 , 6 ). Previously, we demonstrated using immunofluorescence (IF) that the profile of 53BP1 expression, including the number of NF, which reflects the DDR, is clearly altered during

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0630
Volume/Issue:
Volume 10: Issue 3
Open access
Stavroula Stavrou Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Michael Gratz Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Eileen Tremmel Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Christina Kuhn Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Simone Hofmann Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Helene Heidegger Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Mina Peryanova Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Kerstin Hermelink Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Stefan Hutter Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Bettina Toth Department of Gynaecological Endocrinology and Reproductive Medicine, Medical University Innsbruck, Innsbruck, Austria

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Doris Mayr Department of Pathology, Hospital of the LMU, Munich, Germany

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Sven Mahner Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Udo Jeschke Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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Aurelia Vattai Department of Gynecology and Obstetrics, Hospital of the LMU, Munich, Germany

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using Bio-Rad Quantity One software (Bio-Rad Laboratories). Double-immunofluorescence To determine the cells that express TAAR1 and ODC in the decidua, double-immunofluorescence analysis with markers for extravillous trophoblast (EVT) and

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0272
Volume/Issue:
Volume 7: Issue 2
Open access
Antonina Khoruzhenko Institute of Interdisciplinary Research (IRIBHM), Université libre de Bruxelles, Brussels, Belgium
Institute of Molecular Biology and Genetics, NAS of Ukraine, Kiev, Ukraine

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Françoise Miot Institute of Interdisciplinary Research (IRIBHM), Université libre de Bruxelles, Brussels, Belgium

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Claude Massart Institute of Interdisciplinary Research (IRIBHM), Université libre de Bruxelles, Brussels, Belgium

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Jacqueline Van Sande Institute of Interdisciplinary Research (IRIBHM), Université libre de Bruxelles, Brussels, Belgium

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Jacques Emile Dumont Institute of Interdisciplinary Research (IRIBHM), Université libre de Bruxelles, Brussels, Belgium

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Renaud Beauwens Laboratory of Physiology and Pharmacology, Université libre de Bruxelles, Brussels, Belgium

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Alain Boom Laboratoire d’Histologie, de Neuroanatomie et de Neuropathologie, Université libre de Bruxelles, Brussels, Belgium

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follicles retain their functional activity during at least 2 weeks, it is possible to carry out morphological/immunofluorescence analysis of cultured follicles. In the present work, we have implemented and characterized a method of cultivation of rat

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0169
Volume/Issue:
Volume 10: Issue 5
Open access
Shaomin Shi Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, China

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Xinghua Chen Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, China

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Wen Yu Department of Immunology, School of Medicine, Yangtze University, Jingzhou, China

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Xiaolan Ke Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, China

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Tean Ma Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, China

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color (ZLI-9018, Zsbio, Beijing, China) development for 10 min, and hematoxylin for 10 s, followed by dehydration, transparency, blocking, and microscopic examination. Immunofluorescence Paraffin sections were dewaxed and hydrated, antigen

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0284
Volume/Issue:
Volume 12: Issue 10
Open access
Michael J Gratz Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Stavroula Stavrou Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Christina Kuhn Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Simone Hofmann Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Kerstin Hermelink Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Helene Heidegger Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Stefan Hutter Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Doris Mayr Department of Pathology, University Hospital, LMU Munich, Munich, Germany

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Sven Mahner Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Udo Jeschke Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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Aurelia Vattai Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

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 0.1 nM 65 45 92 DDC protein expression in BeWo cells after stimulation with T 1 AM  Control 75 61 98  0.01 nM 50 43 75  0.1 nM 60 50 66 DDC, l -dopa decarboxylase. Double-immunofluorescence

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0126
Volume/Issue:
Volume 7: Issue 5
Open access
Beril Erdem Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey

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Angela Schulz Rudolf Schönheimer Institute of Biochemistry, Faculty of Medicine, Leipzig University, Leipzig, Germany

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Emel Saglar Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey

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Ferhat Deniz Department of Endocrinology, SBÜ Sultan Abdülhamid Han Teaching Hospital, Istanbul, Turkey

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Torsten Schöneberg Rudolf Schönheimer Institute of Biochemistry, Faculty of Medicine, Leipzig University, Leipzig, Germany

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Hatice Mergen Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey

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sequences were N- and C-terminal epitope-tagged (N-terminal: hemagglutinin (HA)-tag; C-terminal: FLAG-tag) ( 10 , 11 ). For immunofluorescence studies, all mutants and the WT receptor were fused to the EGFP sequence right after the AVPR2 coding sequence and

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0236
Volume/Issue:
Volume 7: Issue 1
Open access
Yiqiong Ma Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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Zhaowei Chen Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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Yu Tao Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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Jili Zhu Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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Hongxia Yang Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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Wei Liang Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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Guohua Ding Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China

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increase. Immunofluorescence assay Frozen kidney sections were blocked with 5% bovine serum albumin (BSA) for 30 min at 37°C. The sections were incubated with a mixture of primary antibodies (AKAP1 rabbit monoclonal antibody, 1:100, Cell Signaling

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0234
Volume/Issue:
Volume 8: Issue 8
Open access
Anne-Sophie C A M Koning Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Philippe C Habets Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Marit Bogaards Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Jan Kroon Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Hanneke M van Santen Department of Pediatric Endocrinology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
Department of Pediatric Neuro-Oncology, Prinses Máxima Centrum, Utrecht, The Netherlands

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Judith M de Bont Department of Pediatric Neuro-Oncology, Prinses Máxima Centrum, Utrecht, The Netherlands

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Onno C Meijer Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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wells. Immunofluorescence staining Paraffin-embedded hippocampal sections of 5 µm were mounted on coated slides (Starfrost), deparaffinized in histomount (National Diagnostics, Nottingham, UK) and rehydrated in a graded ethanol series. Antigen

Article Type:
Research Article
Collections:
Cardiovascular
DOI:
https://doi.org/10.1530/EC-21-0425
Volume/Issue:
Volume 11: Issue 3
Open access
Ying Xu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Lei Li Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Jihong Zheng Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Meng Wang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Bopei Jiang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Yue Zhai Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Liumei Lu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Cong Zhang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Zhe Kuang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Xiaomei Yang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Li-Na Jin Department of Hematology, Changzheng Hospital, Naval Medical University, Shanghai, China

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Gufa Lin Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Chao Zhang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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. Direct protein interactions between xlMC3Rs and xlMRAPs were confirmed by co-immunoprecipitation (Co-IP) and immunofluorescence assays in vitro . xlMRAPs exhibited a great impact on the cAMP production of xlMC3R.L/S stimulated by α-MSH. Additionally

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0179
Volume/Issue:
Volume 10: Issue 11
Open access
Zuyao Chen The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China
The First Affiliated Hospital, Department of Otorhinolaryngology, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Xiaolin Zhong The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China
The First Affiliated Hospital, Department of Endocrinology and Metabolism, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Weiqiang Tang The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Min Xia The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Chang Liu Department of Endocrinology and Metabolism, The First People's Hospital of Chenzhou, Chenzhou, China

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Yinping Guo The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Yan Yi The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Qingshan Jiang The First Affiliated Hospital, Department of Otorhinolaryngology, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Xuyu Zu The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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Jing Zhong The First Affiliated Hospital, Institute of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China

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microscope (Olympus). Under the microscope, brown or yellow granules in the cytoplasm or nucleus are positive staining of immunohistochemistry. Immunofluorescence staining B-CPAP and CAL-62 cells were cultured for 24 h in 6-well plates. Cells were

Article Type:
Research Article
Collections:
Thyroid
DOI:
https://doi.org/10.1530/EC-23-0014
Volume/Issue:
Volume 12: Issue 5
Open access