the HAM-RS2 ( P =0.036). The A-V sampling across the forearm muscle also showed a trend for higher glucose uptake with HAM-RS2 compared with the placebo ( P =0.077; Fig. 1 B). Figure 1 Postprandial glucose concentrations (A), glucose flux into the
C L Bodinham, L Smith, E L Thomas, J D Bell, J R Swann, A Costabile, D Russell-Jones, A M Umpleby, and M D Robertson
Leanne Hodson and Fredrik Karpe
hyperinsulinaemia are often said to have ‘insulin resistance’ ( 4 , 5 ). Here we discuss the direct and indirect effects of insulin on intrahepatic processes (e.g. fatty acid synthesis) and systemic processes (e.g. the regulation of fatty acid flux from adipose
Nicolai Preisler, Pascal Laforêt, Karen Lindhardt Madsen, Edith Husu, Christoffer Rasmus Vissing, Gitte Hedermann, Henrik Galbo, Christopher Lindberg, and John Vissing
Pompe disease (glycogenosis type II) is caused by lysosomal alpha-glucosidase deficiency, which leads to a block in intra-lysosomal glycogen breakdown. In spite of enzyme replacement therapy, Pompe disease continues to be a progressive metabolic myopathy. Considering the health benefits of exercise, it is important in Pompe disease to acquire more information about muscle substrate use during exercise.
Seven adults with Pompe disease were matched to a healthy control group (1:1). We determined (1) peak oxidative capacity (VO2peak) and (2) carbohydrate and fatty acid metabolism during submaximal exercise (33 W) for 1 h, using cycle-ergometer exercise, indirect calorimetry and stable isotopes.
In the patients, VO2peak was less than half of average control values; mean difference −1659 mL/min (CI: −2450 to −867, P = 0.001). However, the respiratory exchange ratio increased to >1.0 and lactate levels rose 5-fold in the patients, indicating significant glycolytic flux. In line with this, during submaximal exercise, the rates of oxidation (ROX) of carbohydrates and palmitate were similar between patients and controls (mean difference 0.226 g/min (CI: 0.611 to −0.078, P = 0.318) and mean difference 0.016 µmol/kg/min (CI: 1.287 to −1.255, P = 0.710), respectively).
Reflecting muscle weakness and wasting, Pompe disease is associated with markedly reduced maximal exercise capacity. However, glycogenolysis is not impaired in exercise. Unlike in other metabolic myopathies, skeletal muscle substrate use during exercise is normal in Pompe disease rendering exercise less complicated for e.g. medical or recreational purposes.
Ashley N Reeb, Andrea Ziegler, and Reigh-Yi Lin
-CO2 cell lines. Stable clones of WRO, FTC-238, and TT2609-CO2 cells expressing luciferase were serially diluted. Luciferin substrate was added to each well 10 min before imaging and the plate was imaged to obtain total flux (p/s) per cell line using
Jiwen Yang, Yayin Huang, Bohan Dong, and Yunhai Dai
stained and counted under a microscope. Aerobic glycolysis detection The real-time extracellular acidification rate (ECAR) was detected through an XF96 metabolic flux analyzer (Seahorse Biosciences, Billerica, MA, USA) ( 27 ). TPC-1 and BHT-101
M Feller R Fernandez M Fernandez Oliveira F Ferrau F Ferraù H Ferrero L Figueroa S Filetti C Fiori S Fischli J Flannick A Fletcher E Fliers G Flux C Follin S Forbes P A Foster L Fozzatti M Fragoso K Frank
Espen Nordheim and Trond Geir Jenssen
back-transportation of sodium and glucose from the renal tubular system ( 6 ). Sodium-glucose-co-transporter-2 (SGLT2) contributes to 90% of this transportation reducing distal tubular flux of glucose and sodium. Due to reduced sodium flux in the loop
Mengxue Yang, Bowen Sun, Jianhui Li, Bo Yang, Jie Xu, Xue Zhou, Jie Yu, Xuan Zhang, Qun Zhang, Shan Zhou, and Xiaohua Sun
microbial diversity. Statistical analysis The original high-flux sequencing data were initially screened according to the sequence quality. The sequences were identified and then assigned to corresponding samples according to the primer and barcode
Aasem Saif, Shrook Mousa, Maha Assem, Nashwa Tharwat, and Alaa Abdelhamid
same skilled sonographer. Endothelial function has been assessed by measuring the percent of change in blood flow following heat-mediated vasodilation using laser Doppler flowmetry (Pen Flux System 5000, Perimed AB) in patients and controls. Skin
Po-Chung Cheng and Chia-Hung Kao
prevention of diabetic CHD is an essential component of diabetes management guidelines ( 5 , 6 ). Diabetic dyslipidemia has several unique features. Insulin resistance increases the flux of free fatty acids to the liver, which leads to excessive plasma