the HAM-RS2 ( P =0.036). The A-V sampling across the forearm muscle also showed a trend for higher glucose uptake with HAM-RS2 compared with the placebo ( P =0.077; Fig. 1 B). Figure 1 Postprandial glucose concentrations (A), glucose flux into the
C L Bodinham, L Smith, E L Thomas, J D Bell, J R Swann, A Costabile, D Russell-Jones, A M Umpleby and M D Robertson
Nicolai Preisler, Pascal Laforêt, Karen Lindhardt Madsen, Edith Husu, Christoffer Rasmus Vissing, Gitte Hedermann, Henrik Galbo, Christopher Lindberg and John Vissing
Pompe disease (glycogenosis type II) is caused by lysosomal alpha-glucosidase deficiency, which leads to a block in intra-lysosomal glycogen breakdown. In spite of enzyme replacement therapy, Pompe disease continues to be a progressive metabolic myopathy. Considering the health benefits of exercise, it is important in Pompe disease to acquire more information about muscle substrate use during exercise.
Seven adults with Pompe disease were matched to a healthy control group (1:1). We determined (1) peak oxidative capacity (VO2peak) and (2) carbohydrate and fatty acid metabolism during submaximal exercise (33 W) for 1 h, using cycle-ergometer exercise, indirect calorimetry and stable isotopes.
In the patients, VO2peak was less than half of average control values; mean difference −1659 mL/min (CI: −2450 to −867, P = 0.001). However, the respiratory exchange ratio increased to >1.0 and lactate levels rose 5-fold in the patients, indicating significant glycolytic flux. In line with this, during submaximal exercise, the rates of oxidation (ROX) of carbohydrates and palmitate were similar between patients and controls (mean difference 0.226 g/min (CI: 0.611 to −0.078, P = 0.318) and mean difference 0.016 µmol/kg/min (CI: 1.287 to −1.255, P = 0.710), respectively).
Reflecting muscle weakness and wasting, Pompe disease is associated with markedly reduced maximal exercise capacity. However, glycogenolysis is not impaired in exercise. Unlike in other metabolic myopathies, skeletal muscle substrate use during exercise is normal in Pompe disease rendering exercise less complicated for e.g. medical or recreational purposes.
Ashley N Reeb, Andrea Ziegler and Reigh-Yi Lin
-CO2 cell lines. Stable clones of WRO, FTC-238, and TT2609-CO2 cells expressing luciferase were serially diluted. Luciferin substrate was added to each well 10 min before imaging and the plate was imaged to obtain total flux (p/s) per cell line using
Mengxue Yang, Bowen Sun, Jianhui Li, Bo Yang, Jie Xu, Xue Zhou, Jie Yu, Xuan Zhang, Qun Zhang, Shan Zhou and Xiaohua Sun
microbial diversity. Statistical analysis The original high-flux sequencing data were initially screened according to the sequence quality. The sequences were identified and then assigned to corresponding samples according to the primer and barcode
Aasem Saif, Shrook Mousa, Maha Assem, Nashwa Tharwat and Alaa Abdelhamid
same skilled sonographer. Endothelial function has been assessed by measuring the percent of change in blood flow following heat-mediated vasodilation using laser Doppler flowmetry (Pen Flux System 5000, Perimed AB) in patients and controls. Skin
Yiqiong Ma, Zhaowei Chen, Yu Tao, Jili Zhu, Hongxia Yang, Wei Liang and Guohua Ding
the mitochondrial outer membrane Na + /Ca2 + transporter Ncx3, thereby stabilizing mitochondrial calcium flux and alleviating cellular damage caused by hypoxia ( 34 ). Deleting AKAP1 increases mitochondrial ROS production and aggravates myocardial
Sayaka Kawano, Yukiko Kawagoe, Kenji Kuwasako, Satoshi Shimamoto, Koji Igarashi, Mariko Tokashiki, Kazuo Kitamura and Johji Kato
Nakao K Minami T Yamada C Ueshima K . The effects of super-flux (high performance) dialyzer on plasma glycosylated pro-B-type natriuretic peptide (proBNP) and glycosylated N-terminal proBNP in end-stage renal disease patients on dialysis
Peng Fan, Chao-Xia Lu, Di Zhang, Kun-Qi Yang, Pei-Pei Lu, Ying Zhang, Xu Meng, Su-Fang Hao, Fang Luo, Ya-Xin Liu, Hui-Min Zhang, Lei Song, Jun Cai, Xue Zhang and Xian-Liang Zhou
reabsorption by paracellular flux and potassium secretion by the renal outer medullary K + channels ( 13 ). Three homologous subunits share 30–40% identity, and each one comprises an extracellular loop, two transmembrane domains and a short intracellular N
Meena Asmar, Ali Asmar, Lene Simonsen, Flemming Dela, Jens Juul Holst and Jens Bülow
of Bente Matthiesen is gratefully acknowledged. References 1 Frayn KN . Adipose tissue as a buffer for daily lipid flux . Diabetologia 2002 45 . ( https://doi.org/10.1007/s00125-002-0873-y ) 12242452 2 Samra JS Simpson EJ Clark
Wang Chengji and Fan Xianjin
vascular inflammation and increased proinflammatory cytokines ( 35 ). In diabetes, PKC is activated by advanced glycation end (AGE) products and polyol pathway flux ( 36 ). Also, chronic hyperglycemia stimulates synthesis of DAG and activates DAG