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Masafumi Tetsuka Department of Life and Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan

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Misato Tanakadate Department of Life and Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan

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factors involved in these events, to clarify its physiological role. Therefore, in this study, we examined changes in the activity of HSD11B in the bovine cumulus-oocyte complex during maturation and subsequent fertilization and analyzed the expression

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Yufen Zhao College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Erge Namei College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Bingxue Yang College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Xiangnan Bao Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, PR China
National Center of Technology Innovation for Dairy Industry, Hohhot, PR China

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Wei Sun Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, PR China

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Gerile Subudeng College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Guifang Cao College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Haijun Li College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Gui Wang Animal Genetic Breeding and Reproduction Research Center, Hetao College, Bayannur, PR China

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) or luteinizing hormone (LH) in rats ( 18 ) results in a transient increase in the amount of phosphorylated CX43 protein in cumulus–oocyte complexes (COCs). The intra-oocyte cAMP level is elevated immediately after stimulation with FSH ( 17 ) or LH

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Arpna Sharma Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany

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Vijay Simha Baddela Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany

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Frank Becker Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany

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Dirk Dannenberger Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany

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Torsten Viergutz Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany

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Jens Vanselow Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany

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excessive lipid mobilization has been associated with metabolic and reproductive disorders ( 3 ) affecting both the cumulus oocyte complex morphology and embryo viability ( 4 ). Elevated plasma levels of PA, SA, OA and of linoleic acid have been shown in

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Hui Long Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

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Yanhong Nie Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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Li Wang Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

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Yong Lu Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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Yan Wang Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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Yijun Cai Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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Zhen Liu Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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Miaomiao Jia Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

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Qifeng Lyu Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

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Yanping Kuang Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

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Qiang Sun Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

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larger than 3 mm in diameter. 33–38 h after hCG administration, monkeys were anesthetized with tiletamine hydrochloride (4 to 6 mg/kg, Zoletil 50; Virbac S.A.) intramuscularly, and cumulus-oocyte complexes were collected by laparoscopic follicular

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Wioletta Pijacka
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Morag G Hunter
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Fiona Broughton Pipkin School of Biosciences, New Maternity Unit, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK

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Martin R Luck
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91523 and ab91522 (Abcam). Kidney was used as a positive control. Oocyte collection and in vitro maturation Bovine ovaries were obtained from a local abattoir and transported to the laboratory in thermal containers containing 1× PBS at 39 °C. Cumulus–oocyte

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Rosalie Cabry Amiens University, Amiens, Haut-de-France, France

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Philippe Merviel Brest University, Brest, Bretagne, France

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Aicha Madkour Mohammed V University of Rabat, Reproductive Medicine, Rabat, Morocco

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Elodie Lefranc Amiens University, Amiens, Haut-de-France, France

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Florence Scheffler Amiens University, Amiens, Haut-de-France, France

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Rachel Desailloud Amiens University, Amiens, Haut-de-France, France

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Véronique Bach Amiens University, Amiens, Haut-de-France, France

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Moncef Benkhalifa Amiens University, Amiens, Haut-de-France, France

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in oestradiol levels, affecting oocyte maturation ( 51 ). Furthermore, PCB might damage the ovarian reserve, the follicular response to administered gonadotropins, and oocyte maturation ( 51 ). Indeed, exposure of immature porcine cumulus-oocyte

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