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Department of Endocrinology and Diabetes, The First Affiliated Hospital, Xiamen University, Xiamen, China
Fujian Province Key Laboratory of Diabetes Translational Medicine, Xiamen, China
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), has been proven to be a reliable index for the evaluation of abdominal fat dysfunction in the Chinese population ( 24 ). The CVAI was proposed for the first time from a cross-sectional study with 485 subjects and was further validated in a study with
eXtraOrdinarY Kids Clinic, Children's Hospital Colorado, Aurora, Colorado, USA
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eXtraOrdinarY Kids Clinic, Children's Hospital Colorado, Aurora, Colorado, USA
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eXtraOrdinarY Kids Clinic, Children's Hospital Colorado, Aurora, Colorado, USA
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eXtraOrdinarY Kids Clinic, Children's Hospital Colorado, Aurora, Colorado, USA
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. Methods Overall study design This was a cross-sectional study of untargeted metabolite concentrations in the plasma of 31 males with KS compared to 33 controls selected for similar age, pubertal stage, and body mass index (BMI). The study was
K.G. Jebsen Center for Autoimmune Disorders, University of Bergen, Bergen, Norway
Department of Medicine, Haukeland University Hospital, Bergen, Norway
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Department of Gynecology and Obstetrics, Haukeland University Hospital, Bergen, Norway
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K.G. Jebsen Center for Autoimmune Disorders, University of Bergen, Bergen, Norway
Department of Medicine, Haukeland University Hospital, Bergen, Norway
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Department of Endocrinology, Metabolism and Diabetes, Karolinska University Hospital, Stockholm, Sweden
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Department of Sleep, Landspitali University Hospital Reykjavík, Reykjavik, Iceland
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Allergy and Lung Health Unit, Melbourne School of Population and Global Health, The University of Melbourne, Melbourne, Victoria, Australia
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Albacete Faculty of Medicine, Castilla-La Mancha University, Albacete, Spain
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The National Research Center for the Working Environment, Copenhagen, Denmark
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K.G. Jebsen Center for Autoimmune Disorders, University of Bergen, Bergen, Norway
Department of Medicine, Haukeland University Hospital, Bergen, Norway
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menopausal age and information on health-, lifestyle factors and reproductive hormone levels. Methods Study population In this retrospective cross-sectional study, the study population consisted of the second follow-up of the European Community
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. Methods Study population This study was part of the European dsd-LIFE study ( https://www.dsd-life.eu/ ), a non-interventional, clinical, cross-sectional study ( 8 ). The purpose of the study was to investigate and compare the long-term outcomes of
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CUNY Graduate School of Public Health and Health Policy, New York, New York, USA
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Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA
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Objectives
Recently, an estradiol immunoassay manufacturer (Beckman Coulter, USA) issued an ‘important product notice’ alerting clinical laboratories that their assay (Access Sensitive Estradiol) was not indicated for patients undergoing exogenous estradiol treatment. The objective of this analysis was to evaluate immunoassay bias relative to liquid chromatography tandem mass spectrometry (LC-MS/MS) in transgender women and to examine the influence of unconjugated estrone on measurements.
Design
Cross-sectional secondary analysis.
Methods
Estradiol concentrations from 89 transgender women were determined by 3 immunoassays (Access Sensitive Estradiol (‘New BC’) and Access Estradiol assays (‘Old BC’), Beckman Coulter; Estradiol III assay (‘Roche’), Roche Diagnostics) and LC-MS/MS. Bias was evaluated with and without adjustment for estrone concentrations. The number of participants who shifted between three estradiol concentration ranges for each immunoassay vs LC-MS/MS (>300 pg/mL, 70–300 pg/mL, and <70 pg/mL) was calculated.
Results
The New BC assay had the largest magnitude overall bias (median: −34%) and was −40%, −22%, and −10%, among participants receiving tablet, patch, or injection preparations, respectively. Overall bias was −12% and +17% for the Roche and Old BC assays, respectively. When measured with the New BC assay, 18 participants shifted to a lower estradiol concentration range (vs 9 and 10 participants based on Roche or Old BC assays, respectively). Adjustment for estrone did not minimize bias.
Conclusions
Immunoassay measurement of estradiol in transgender women may lead to falsely decreased concentrations that have the potential to affect management. A multidisciplinary health care approach is needed to ensure if appropriate analytical methods are available.
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Objective
Vitamin D plays an important role during pregnancy. The aim was to compare vitamin D status in a group of singleton (SP) and twin pregnancies (TP) using two diagnostic methods: chemiluminescence immunoassay (CLIA) and liquid chromatography with tandem mass spectrometry (LC-MS/MS).
Design
This is a cross-sectional study.
Methods
The study was conducted in the population of SP and TP at the gestational age above 20 + 0 at the Bielanski Hospital in Warsaw, Poland, between October 2020 and January 2023. All patients had their venous blood samples collected and were given an original survey containing questions on demography and vitamin D supplementation.
Results
The study group included 53 Caucasian women with SP and 78 with TP aged from 21 to 47. Considering LC-MS/MS, patients with TP had lower concentrations of 25-hydroxyvitamin D (25(OH)D) than patients with SP. However, no significant difference was observed in the frequency of the occurrence of vitamin D deficiency (25(OH)D < 30 ng/mL). In both groups, the levels obtained with CLIA were significantly lower than in case of LC-MS/MS, however, strongly correlated. The intermethod agreement accounted for 52.4% and the Cohen’s kappa coefficient was 0.142.
Conclusions
The concentration of 25(OH)D in pregnant women depends on the type of gestation (SP/TP) and on the diagnostic methods used (CLIA/LC-MS/MS). Based on LC-MS/MS, the incidence of vitamin D deficiency was low in our group and no differences occurred in its frequency between SP and TP. The intermethod agreement between CLIA and LC-MS/MS on the detection of vitamin D deficiency was low.
Significance statement
This is the first study to compare the concentration of 25(OH)D levels between SP and TP using two methods: CLIA and the gold standard – LC-MS/MS. Based on LC-MS/MS, a low incidence of vitamin D deficiency was observed in our group, in which the vast majority of patients took cholecalciferol supplements. Moreover, there were no differences in its frequency between SP and TP. However, the 25(OH)D level was significantly lower in TP. The intermethod agreement between CLIA and LC-MS/MS on the detection of vitamin D deficiency was low, which is associated with substantial clinical implications.
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systematic review that the vast majority of studies showed an association between low testosterone concentrations and high CRP levels in men ( 2 ). Due to the cross-sectional design of studies, it is challenging to understand the direction of these
Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark
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Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark
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Department of Geriatrics, Aalborg University Hospital, Aalborg, Denmark
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Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark
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all observational and mainly cross-sectional or case–control designs. The methods used for assessment of thyroid disease varied considerably, and three studied relied on registered diagnosis of thyroid disease rather than actual biochemical
Alfred Health, Melbourne, Victoria, Australia
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Department of General Practice, Melbourne Medical School, The University of Melbourne, Victoria, Australia
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supraphysiological testosterone exposure in a literature review, and then present a scoping review of cross-sectional and prospective studies (cohort and randomised trials) that examine the recovery of hypogonadism, categorising outcomes into physical recovery
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significantly reduced in reproductive-age females with FA and DC/TBD ( 29 , 30 ). The goal of the present study was to assess whether AMH levels are reduced in males with FA, DC/TBD, and DBA. Materials and methods This cross-sectional study includes
