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Paal Methlie, Steinar Hustad, Ralf Kellman, Bjørg Almås, Martina M Erichsen, Eystein S Husebye and Kristian Løvås

factors likely to slow its adoption. The development of simple, reliable, and cost- and labour-efficient methods is, therefore, important. Liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is a highly selective mode of detection. It

Open access

Sandra R Dahl, Ingrid Nermoen, Ingeborg Brønstad, Eystein S Husebye, Kristian Løvås and Per M Thorsby

(LC–MS/MS) has emerged as the method of choice for determination of steroid hormones. Advantages include its superior specificity compared to immunoassays, the possibility for multiplexing and low sample volume ( 10 ). This enables simultaneous

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T P Parikh, B Stolze, Y Ozarda, J Jonklaas, K Welsh, L Masika, M Hill, A DeCherney and S J Soldin

participants by an LC–MS/MS method primarily developed for diagnosis and subtyping of patients with disorders of adrenal function and steroidogenesis. Subjects and methods Subjects and specimens Subjects included 182 healthy patients all providing

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Marloes L P Langelaan, Jérôme M H Kisters, Mirjam M Oosterwerff and Arjen-Kars Boer

concentrations in healthy individuals would hamper the identification of AI ( 1 ). However, novel liquid chromatography tandem-mass spectrometry (LC–MS/MS) techniques have made it possible to increase accuracy and sensitivity in the determination of salivary

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M P Schuijt, C G J Sweep, R van der Steen, A J Olthaar, N M M L Stikkelbroeck, H A Ross and A E van Herwaarden

chromatography tandem mass spectrometry (ED-LC-MS/MS). LC-MS/MS enables the accurate, specific and matrix-independent measurement of testosterone with direct calibration. Equilibrium dialysis enables to separate the free- from protein-bound testosterone with

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Filippo Ceccato, Elisa Selmin, Chiara Sabbadin, Miriam Dalla Costa, Giorgia Antonelli, Mario Plebani, Mattia Barbot, Corrado Betterle, Marco Boscaro and Carla Scaroni

form, to ensure a correct home sampling ( 13 , 19 ). Salivary F and E were routinely measured by LC-MS/MS with an automated sample preparation, as previously described, utilizing an Agilent HPLC series 1200, with a triple quadrupole mass spectrometer

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Jonneke J Hollanders, Bibian van der Voorn, Noera Kieviet, Koert M Dolman, Yolanda B de Rijke, Erica L T van den Akker, Joost Rotteveel, Adriaan Honig and Martijn J J Finken

-grade methanol at 25°C for 18 h. These extracts were subsequently centrifuged and cleaned using solid phase extraction. GC concentrations were quantified by liquid chromatography–tandem mass spectrometry LC–MS/MS (Waters XEVO-TQ-S system, Waters Corporation

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Thomas Reinehr, Alexandra Kulle, Juliane Rothermel, Caroline Knop-Schmenn, Nina Lass, Christina Bosse and Paul-Martin Holterhus

, mineralocorticoids and sex hormones are a challenge because of the relatively low specificity of ELISA and RIA. Hence, the state-of-the-art method is a liquid chromatography–tandem mass spectrometry (LC–MS/MS) steroid profiling method ( 7 ), which we have used in

Open access

Thomas Reinehr, Alberto Sánchez-Guijo, Nina Lass and Stefan A Wudy

-of-the-art liquid chromatography–tandem mass spectrometry (LC–MS/MS) ( 20 ). This method can quantify 11 sulfated steroids simultaneously, providing a powerful tool to understand the sulfated steroidome in human blood ( 21 ). We hypothesized that sulfated steroids

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Amalie Carlsson, Kaspar Sørensen, Anna-Maria Andersson, Hanne Frederiksen and Anders Juul

one out of nine environmental phenols measured by liquid chromatography-tandem mass spectrometry (LC–MS/MS) with prior enzymatic deconjugation ( 19 , 20 ). For the present study, first morning urine collected from the first of the two consecutive