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rats. A comprehensive GC-MS untargeted metabolomics approach was applied to urine samples taken from all rats. Materials and methods Animals and induction of diabetes Induction of diabetes Thirty male Sprague–Dawley rats, weighing 200
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Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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(GC–MS) using a Perkin Elmer Clarus 500 system with an OV-1 column (Perkin Elmer, Beaconsfield, Buckinghamshire, UK). Quantification was based on data obtained in cyclic scan mode. Additional procedures were carried out using published protocols for
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ultrasonography – L1 L , or both – L1 AL ) where defined as liver disease – L1 as compared to L0 – without markers of liver disease. Gas chromatography-mass spectrometry (GC-MS) of urinary steroids Steroid metabolites in 24-h urine samples were analyzed by
Sahlgrenska Osteoporosis Centre, Center for Bone and Arthritis Research (CBAR), Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden
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Sahlgrenska Osteoporosis Centre, Center for Bone and Arthritis Research (CBAR), Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden
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Sahlgrenska Osteoporosis Centre, Center for Bone and Arthritis Research (CBAR), Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden
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Department of Endocrinology, Skaraborg Central Hospital, Skövde, Sweden
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assay using gas chromatography combined with tandem MS (GC-MS/MS) for measurements of sex steroids in analytically challenging matrices with low steroid levels such as serum and tissues from rodents as well as human serum ( 14 , 15 ). Recently, we used
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Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, UK
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. Glucocorticoid metabolites were measured by gas chromatography-tandem mass spectrometry (GC-MS/MS) ( 39 ). Samples were analyzed in 15 batches. Creatinine concentrations were measured by the Jaffé method ( 40 ). The sum of cortisol metabolites divided by
Department of Endocrinology, Department of Molecular Medicine and Surgery, Metabolism and Diabetology, Karolinska University Hospital, 171 76 Stockholm, Sweden
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metabolites LMCs from the serum samples were extracted and analysed by gas chromatography-coupled mass spectroscopy (GC/MS) (Pegasus III time-of-flight mass spectrometer, GC/TOFMS (Leco Corp., St Joseph, MI, USA)) according to a method developed previously
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have used immunoassays which have the problem of steroid cross reactivity (20, 21) . We have addressed this problem by utilising gas chromatography-mass spectrometry (GC-MS) (22) . In addition, we have measured steroids in 24 h urine collections
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development of obesity-associated hypertension requires various concerted steps that could result in an increased availability of bioactive glucocorticoids. Therefore, in the present study using targeted gas chromatography-mass spectrometry (GC-MS) steroid
Department of Pediatrics, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Reproduction & Development Research Institute, de Boelelaan, Amsterdam, The Netherlands
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Department of Pediatrics, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Reproduction & Development Research Institute, de Boelelaan, Amsterdam, The Netherlands
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Department of Pediatrics, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Reproduction & Development Research Institute, de Boelelaan, Amsterdam, The Netherlands
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-mass spectrometry (GC-MS) analysis. Briefly, free and conjugated steroids were extracted from up to 5 mL of urine by solid phase extraction and the conjugates were enzymatically hydrolyzed. After recovery of hydrolyzed steroids by solid phase extraction, known
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of this article). GC–MS/MS analysis of steroids Steroids from the HFA at GW9–10 ( n = 5) and GW11–12 ( n = 7) were extracted twice with diethyl ether and prepared for analysis as described earlier ( 17 ). Briefly, tissue samples (from 0.5 to