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Jairo Arturo Pinzón-Cortés, Angelina Perna-Chaux, Nicolás Steven Rojas-Villamizar, Angélica Díaz-Basabe, Diana Carolina Polanía-Villanueva, María Fernanda Jácome, Carlos Olimpo Mendivil, Helena Groot, and Valeriano López-Segura

methylation and demethylation processes in diabetes may be differently affected depending on the local conditions and tissue exposed to the disease ( 17 ). Concerning the source of the observed differences in DNA methylation, they can be attributed to

Open access

A H Ludwig-Slomczynska, S Borys, M T Seweryn, J Hohendorff, P Kapusta, B Kiec-Wilk, E Pitera, P P Wolkow, and M T Malecki

- and post-treatment). Bisulfite conversion was performed using 500 ng of DNA with EZ DNA Methylation-Direct Kit (Zymo, Irvine, CA, USA). The next steps were performed according to the Illumina protocol of Methylation EPIC kit (Illumina, San Diego, CA

Open access

Ya-Fen Hu, Lin Hua, Xiu Tuo, Ting-Ting Shi, Yi-Lin Yang, Yun-Fu Liu, Zhong-Yu Yan, and Zhong Xin

outer edge of the orbit was at the lowest point. Identification of candidate genes In a previous study, a genome-scale screening of DNA methylation was performed on the peripheral blood sample from six patients with GO and six controls

Open access

Jessica S Jarmasz, Yan Jin, Hana Vakili, and Peter A Cattini

chromatin that influence gene expression ( 23 ). The methylation of cytosine paired with a guanine (CpG) termed ‘DNA methylation’ is one of the many epigenetic mechanisms that govern gene expression where appro­ximately 70–80% of CpG dinucleotides are

Open access

Kristian Almstrup, Hanne Frederiksen, Anna-Maria Andersson, and Anders Juul

epigenetic studies. We have previously published one of the first studies demonstrating changes in DNA methylation patterns with the onset of puberty in healthy children ( 12 ) and identified the promotor of the thyroid hormone receptor interactor 6 gene

Open access

Elham Barazeghi, Per Hellman, Gunnar Westin, and Peter Stålberg

tumors ( 4 ). Promoter DNA methylation studies revealed increased RASSF1A / CTNNB1 methylation associated with metastatic progression, and epigenetic repression of TCEB3C in SI-NETs ( 5 , 6 ). A comprehensive integrated molecular analysis of a large

Open access

Anne Jouinot, Juliane Lippert, Martin Fassnacht, Bruno de La Villeon, Amandine Septier, Mario Neou, Karine Perlemoine, Silke Appenzeller, Mathilde Sibony, Sébastien Gaujoux, Bertrand Dousset, Rossella Libe, Lionel Groussin, Cristina L Ronchi, Guillaume Assié, and Jérôme Bertherat

mutations, chromosome alteration and DNA methylation profile, or at the RNA level, including transcriptome and targeted gene expression profiles. Intratumor heterogeneity of somatic mutations has been reported in many cancer types ( 12 , 13 , 14 ). In a

Open access

Roberto Cosimo Melcangi, Livio Casarini, Marco Marino, Daniele Santi, Samantha Sperduti, Silvia Giatti, Silvia Diviccaro, Maria Grimoldi, Donatella Caruso, Guido Cavaletti, and Manuela Simoni

analysed in duplicate and the percentage of DNA methylation was calculated using the previously described 2 −ΔΔCt method ( 35 ) optimised for DNA methylation analyses ( 36 , 37 ). Briefly, ΔCt values were calculated by subtracting the internal control Ct

Open access

K G Samsom, L M van Veenendaal, G D Valk, M R Vriens, M E T Tesselaar, and J G van den Berg

deletion in 89% SI-NETs with reduced Elongin A3 expression in 77%. – – Fotouhi et al. 2014 33 SI-NETs ( n  = 44) Pyrosequencing, ELISA-based quantification of global DNA methylation, qRT-PCR Methylation was seen in WIF1 (methylation

Open access

Djeda Belharazem, Matthias Kirchner, Franziska Geissler, Peter Bugert, Martin Spahn, Burkhard Kneitz, Hubertus Riedmiller, Christian Sauer, Stefan Küffer, Lutz Trojan, Christian Bolenz, Maurice Stephan Michel, Alexander Marx, and Philipp Ströbel

different between controls and RPE patients ( P =0.06). Bisulfite treatment DNA methylation analyses, starting with 1–2 μg DNA, were carried out using bisulfite treatment and subsequent sequencing as described previously (26) . For PCR amplification of