School of Medicine, Universidad de los Andes, Bogotá, Colombia
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Endocrinology Section, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá, Colombia
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School of Medicine, Universidad de los Andes, Bogotá, Colombia
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School of Medicine, Universidad de los Andes, Bogotá, Colombia
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methylation and demethylation processes in diabetes may be differently affected depending on the local conditions and tissue exposed to the disease ( 17 ). Concerning the source of the observed differences in DNA methylation, they can be attributed to
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University Hospital, Krakow, Poland
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University Hospital, Krakow, Poland
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University Hospital, Krakow, Poland
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University Hospital, Krakow, Poland
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- and post-treatment). Bisulfite conversion was performed using 500 ng of DNA with EZ DNA Methylation-Direct Kit (Zymo, Irvine, CA, USA). The next steps were performed according to the Illumina protocol of Methylation EPIC kit (Illumina, San Diego, CA
Department of Endocrinology, The People’s Hospital of Daxing District, Beijing, China
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outer edge of the orbit was at the lowest point. Identification of candidate genes In a previous study, a genome-scale screening of DNA methylation was performed on the peripheral blood sample from six patients with GO and six controls
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chromatin that influence gene expression ( 23 ). The methylation of cytosine paired with a guanine (CpG) termed ‘DNA methylation’ is one of the many epigenetic mechanisms that govern gene expression where approximately 70–80% of CpG dinucleotides are
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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epigenetic studies. We have previously published one of the first studies demonstrating changes in DNA methylation patterns with the onset of puberty in healthy children ( 12 ) and identified the promotor of the thyroid hormone receptor interactor 6 gene
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tumors ( 4 ). Promoter DNA methylation studies revealed increased RASSF1A / CTNNB1 methylation associated with metastatic progression, and epigenetic repression of TCEB3C in SI-NETs ( 5 , 6 ). A comprehensive integrated molecular analysis of a large
Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Comprehensive Cancer Center Mainfranken, University of Wuerzburg, Wuerzburg, Germany
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Department of Pathology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Digestive and Endocrine Surgery, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Digestive and Endocrine Surgery, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Institute of Metabolism and System Research, University of Birmingham, Birmingham, UK
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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Department of Endocrinology, Assistance Publique Hôpitaux de Paris, Hôpital Cochin, Paris, France
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mutations, chromosome alteration and DNA methylation profile, or at the RNA level, including transcriptome and targeted gene expression profiles. Intratumor heterogeneity of somatic mutations has been reported in many cancer types ( 12 , 13 , 14 ). In a
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Center for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy
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Center for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy
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Department of Medical Specialties, Azienda Ospedaliero-Universitaria di Modena, Modena, Italy
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Center for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy
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Center for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy
Department of Medical Specialties, Azienda Ospedaliero-Universitaria di Modena, Modena, Italy
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analysed in duplicate and the percentage of DNA methylation was calculated using the previously described 2 −ΔΔCt method ( 35 ) optimised for DNA methylation analyses ( 36 , 37 ). Briefly, ΔCt values were calculated by subtracting the internal control Ct
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various diseases, including thyroid cancer ( 3 , 4 ). This regulation includes DNA methylation, chromatin remodeling, histone modifications, and the expression of diverse non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), long ncRNAs (lncRNAs), and
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark
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Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark
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Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark
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Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Gynaecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark
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Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Endocrinology, Aarhus University Hospital, Aarhus, Denmark
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Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark
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chromosome abnormalities,’ ‘Turner syndrome,’ ‘Klinefelter syndrome,’ ‘47,XYY,’ and ‘47,XXX’ as search terms in combination with ‘DNA methylation,’ ‘transcriptome profile,’ ‘epigenetics,’ and ‘genomics’. Relevant articles were obtained and reviewed as well as