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biologically functional metabolite, 1,25 dihydroxy vitamin D3 (1,25(OH) 2 D 3 ), is generated by two successive hydroxylations in the liver by 25 hydroxylase (CYP27A1) and in the kidney by 1α-hydroxylase (1α(OH)ase; CYP27B1). When 1,25(OH) 2 D 3 level reaches
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decreasing serum 25(OH)D and no plateau was found ( 17 ). PTH may up-regulate the expression of the NF-κB ligand receptor activator, inhibit osteoprotegerin mRNA expression, and accelerate bone turnover ( 38 ). In animal models, 1α(OH)ase knockout mice