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Department of Clinical Chemistry, Hematology and Immunology, Noordwest Ziekenhuis, Alkmaar, The Netherlands
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Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Amsterdam UMC location University of Amsterdam, Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands
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always the same for every method designed to measure vitamin D. Second, knowing the pitfalls of the used assay, most often an automated immunoassay or a liquid-chromatography coupled to tandem mass-spectrometry, is important for the interpretation of the
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Center for Healthy Aging, Universitätsklinikum Dresden, Technische Universität Dresden, Dresden, Germany
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decorated by the Nobel committee for his research on the composition of sterols and their connection to vitamins in 1928. The formal mass spectrometry confirmation of this UV-dependent non-enzymatic activation reaction in the skin was not, however, provided
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) Küçükali ( 19 ), 2021 Turkey; NM Cross sectional 162 (84/78) F/M 12–18 84 obese children and 78 healthy children 25(OH)D: Liquid chromatography–tandem mass spectrometry with a commercial kit. PTH: colorimetric method using ready-made kits
Fondazione Italiana Ricerca sulle Malattie dell’Osso (FIRMO Onlus), Florence, Italy
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to a reference procedure (isotope dilution mass spectrometry) and automatized on a Cobas 8000. Serum VDBP was measured by enzyme-linked immuno assay method (R&D Systems). BPA analysis was performed on urine samples using the HPLC/mass spectrometry by
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Alexandridou A Schorr P Stokes CS Volmer DA . Analysis of vitamin D metabolic markers by mass spectrometry: recent progress regarding the ‘gold standard’ method and integration into clinical practice . Mass Spectrometry Reviews 2021 [epub]. ( https
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commercial kits. The serum concentration of 25 hydroxyvitamin D (25(OH)D) was measured by HPLC/mass spectrometry. Cardiovascular events were obtained by phone calls to patients’ homes and by reviewing the hospital records. In the study, we defined
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identification of the metabolite as 1α,25-(OH) 2 D 3 with mass spectrometry. Chemically synthesized 1,25-(OH) 2 D 3 was first produced by Semmler et al. ( 43 ) and made commercially by a group headed by Dr Milan Uskokovic at Hoffmann-La Roche in the early
Department of Nutrition, Institute of Life Sciences, Federal University of Juiz de Fora, Governador Valadares, Minas Gerais, Brazil
Department of Nutrition, Faculty of Health and Medical Sciences, University of Surrey, University of Surrey, Guildford, UK
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, protein binding assays, high-performance liquid chromatography (HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) ( 15 , 52 , 53 ). This variety of methods may partially explain the variability of results. However, there is a consensus
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-off representing an increased risk of vitamin D deficiency ( 1 ). The US survey used a standardised liquid chromatography with tandem mass spectrometry (LC-MS/MS) methodology for measurement of 25(OH)D, whereas the Canadian survey used an immunoassay-based method
School of Nursing, Institute of Clinical Sciences, University of Birmingham, Edgbaston, Birmingham, UK
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DJ . Circulating conjugated and unconjugated vitamin D metabolite measurements by liquid chromatography mass spectrometry . Journal of Clinical Endocrinology and Metabolism 2022 107 435 – 449 . ( https://doi.org/10.1210/clinem/dgab708 ) 3