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value, and low specificity, a rule-out test for thyroid cancer, with ‘benign’ or ‘suspicious’ test results ( 8 ); (ii) ThyroSeq v2 (ThyroSeq; University of Pittsburgh Medical Center, Pittsburgh, PA, USA and Sonic Healthcare, Austin, TX, USA), released in
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patients are diagnosed using commercially available molecular tests. The most widely used tests, Afirma Gene Expression Classifier (Afirma GEC) and ThyroSeq, were developed to reduce the number of thyroidectomies in patients with indeterminate nodules. Most
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. Next-generation sequencing-based ThyroSeq® using FNAC samples was recently reported as the most accurate test for thyroid nodules, which prevents the largest number of diagnostic thyroidectomy cases, avoiding surgery for up to 61% of all Bethesda III
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thyroid malignancies ( 3 , 4 ). To identify and understand the biomarkers of cancer susceptibility is of great clinical value. NGS is a means of learning what is happening behind each thyroid tumor. NGS panels targeting thyroid diseases, like Thyroseq
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in thyroid specimens are commercially available (Thyroseq V3, Afirma GSC, and ThyraMIR) ( 23 ), but these panels are not listed in China. Indeed, they were not developed based on Chinese population data, and available data suggest differences in
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