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-associated protein proliferating cell nuclear antigen (PCNA) and the apoptosis-associated proteins BCL-XL and BAX were measured using respective ELISA kits (Meimian, Jianshu, China) according to the manufacturer instructions. Key ceRNA network construction and
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, and TNF-α and interleukin-6 (IL-6) concentrations were measured using an ELISA kit (R&D Systems) according to the manufacturer’s instructions. For the in vivo experiment, serum TSH, FT3, and FT4 levels of mice were determined using an ELISA kit
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
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German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
Department of Nuclear Medicine, University Hospital Münster, Münster, Germany
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German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
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German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
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German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
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Institute of Pathology, University Hospital Essen, University Duisburg-Essen, Essen, Germany
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German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
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German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
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enzyme-linked immunosorbent assay (ELISA) with an LoQ in our laboratory of 0.09 ng/mL (Medizym® Tg Rem ELISA; Medipan, Blankenfelde-Mahlow, Germany) was used. As described in the current ATA guidelines ( 1 , 8 ), a suppressed Tg <0.2 ng/mL was chosen as
Department of Diabetes & Endocrinology, Skåne University Hospital, Malmö, Sweden
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Department of Diabetes & Endocrinology, Skåne University Hospital, Malmö, Sweden
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Department of Ophthalmology, Skåne University Hospital, Malmö, Sweden
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Department of Diabetes & Endocrinology, Skåne University Hospital, Malmö, Sweden
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-linked immunosorbent assay Cell culture supernatants were harvested, and the protein levels of PGE 2 , IL-1B, IL-6, and IgG were analyzed with commercially available ELISA kits (R & D Systems) according to the manufacturer’s guidelines. Cell culture for flow
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Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
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Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
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performed as follows: The level of neutrophil elastase was measured with the Human PMN-Elastase Platinum ELISA kit (eBioscience, Vienna, Austria); the level of histone–DNA complex was measured with the Cell Death Detection ELISA kit (Roche Diagnostics); the