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collection All blood samples were collected from the abdominal aorta and immediately centrifuged at 3000 g for 10 min at 4°C under anesthesia before sacrifice. Serum IGF-1 levels were measured with ELISA kits (Cloud-Clone Corp., Wuhan, China) according
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Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, Hunan, China
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Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, Hunan, China
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glucose, HbA1c, total cholesterol (TC), HDL-C, LDL-C, and triacylglycerols (TG). For insulin, ghrelin, and LEAP2 measurement, separate blood samples were collected without anticoagulation to obtain serum for ELISA assay. All blood samples were centrifuged
Department of Infectious Diseases, Copenhagen University Hospital – Amager and Hvidovre, Hvidovre, Denmark
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Centre for Physical Activity Research, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
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Centre for Physical Activity Research, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
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Centre for Physical Activity Research, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Center for Clinical Research and Prevention, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Copenhagen, Denmark
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Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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described for the first cohort ( 11 ). For the second COVID-19 cohort, serum IL-6 was measured by microfluidic ELISA (Ella, R&D Systems). To account for measurement bias, the protocol for measurement of gonadal hormones was the same regardless of cohort