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determined by commercial ELISA kits following the manufacturer’s protocol (Jingmei Engineering, Jiangsu Province, China). For all kits, the interassay and intra-assay coefficients of variation were <12 and < 8%, respectively. The minimum detectable
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Department of Cardiology, Yanbian University Hospital, Yanji, China
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tissue wet weight (−80℃ was placed after anticoagulant treatment with aprotinin and EDTA in an ANP radioimmunoassay kit before collection of human, rat perfusate, and mouse plasma). ELISA Plasma from 163 patients was subjected to ELISA experiments
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enzymatically on an analyzer (Hitachi 912; Roche Diagnostics), and LDL cholesterol was calculated by the Friedewald equation. Serum levels of IDOL and PCSK9 were measured using commercially available ELISA kits (CUSABIO® Biotech Co., MD, USA; and Quantikine, R