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Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
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Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
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Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany
Cologne Center for Musculoskeletal Biomechanics (CCMB), Cologne, Germany
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umbilical blood ( 14 ). Since there is an urgent need for a reliable and sensitive method to determine plasma asprosin levels ( 15 ), our laboratory took the initiative to develop a functional and dependable asprosin ELISA ( 16 ). Here, we set out to test
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commercially available immunoassay ELISA kit (Immutopics Inc.). The analytical parameters of the cFGF23 kit were detection limit 1.5 RU/mL and working range 1.5–660 RU/mL (intraassay CV = 1.4–2.4%, interassay CV = 2.4–4.7%) and reference interval 21.6–91 RU
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markers procollagen type 1 amino-terminal-propeptide (P1NP), C-terminal telopeptide of type I collagen (CTX) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) were measured using commercially available ELISA kits from IDS (Frankfurt/Main, Germany). Dkk
PhyMedExp, Université de Montpellier, INSERM, CNRS, Montpellier, France
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PhyMedExp, Université de Montpellier, INSERM, CNRS, Montpellier, France
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CIC INSERM 1411, Hôpital Gui de Chauliac, CHU Montpellier, Montpellier Cedex 5, France
Institut de Génomique Fonctionnelle, CNRS UMR 5203/INSERM U661/Université Montpellier, Montpellier, France
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CIC INSERM 1411, Hôpital Gui de Chauliac, CHU Montpellier, Montpellier Cedex 5, France
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Département de Biochimie, Hôpital Lapeyronie, CHU Montpellier, Montpellier, France
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Département d’Urgence et Post-Urgence Psychiatrique, Hôpital Lapeyronie, CHU Montpellier, Montpellier, France
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Département d’Urgence et Post-Urgence Psychiatrique, Hôpital Lapeyronie, CHU Montpellier, Montpellier, France
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Département Endocrinologie, Nutrition, Diabète, Equipe Nutrition, Diabète, CHU Montpellier, Montpellier, France
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lower than 7%. The intra- and inter-assay CVs for IGF-1 (Immulite 2000 ® IGF-1, Siemens Healthcare Diagnostics) were lower than 6%. The determination of myokines was performed by ELISA assays. Follistatin and myostatin were measured with R
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Diagnostic Clinic, University Hospital of North Norway, Tromso, Norway
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Division of Internal Medicine, University Hospital of North Norway, Tromso, Norway
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,25(OH) 2 D was 20 nmol/L, hence all participants with values under this limit were allocated the value 0 ( n = 27). Serum free 25(OH)D was analyzed by a competitive ELISA assay, cat. no. KAPF 1991, DIAsource ImmunoAssays SA (Louvain-la-Neuve, Belgium
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CACS for each patient at month 12. Serum concentrations of intact FGF23 (iFGF23) (Boster Biological Technology., Ltd., Wuhan, China) and KLA (Immuno-Biological Laboratories Co., Ltd., Gunma, Japan) were measured using ELISA methods with the available
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)D level. 6 Al-Daghri ( 55 ), 2015 Saudi Arabia; NM Cross-sectional 830 F/M 18–50 Apparently healthy individuals ELISA Sufficient vitamin D >50 nmol/L, insufficient: 25–50 nmol/L and deficient: <25 nmol/L Weight, height, BMI, WC