insulin resistant groups, into similar observations in a T2DM cohort. Using an integrated, whole-body approach, stable isotope tracers to differentiate between changes in hepatic and peripheral insulin sensitivity of glucose uptake, body composition
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C L Bodinham, L Smith, E L Thomas, J D Bell, J R Swann, A Costabile, D Russell-Jones, A M Umpleby, and M D Robertson
Nicolai Preisler, Pascal Laforêt, Karen Lindhardt Madsen, Edith Husu, Christoffer Rasmus Vissing, Gitte Hedermann, Henrik Galbo, Christopher Lindberg, and John Vissing
during prolonged (i.e. 1 h) moderate intensity exercise, in patients with Pompe disease. We used cycle-ergometer exercise and stable isotope methodology in combination with indirect calorimetry to determine glucose, glycerol, and fatty acid metabolism
Leanne Hodson and Fredrik Karpe
hepatic DNL to a greater extent than consumption of the same amount of either glucose or fructose alone ( 65 , 66 , 67 , 68 ). Metabolic studies, utilising stable-isotope labelled fructose, have demonstrated that the contribution of ingested fructose to
Ingeborg Brønstad, Lars Breivik, Paal Methlie, Anette S B Wolff, Eirik Bratland, Ingrid Nermoen, Kristian Løvås, and Eystein S Husebye
molecular weight of wt 21OH (56 kDa) were cut out for trypsin treatment. Stable isotope-labelled synthetic peptides (N-terminal replacement of lysine ( 13 C 6 , 15 N 2 ) or arginine ( 13 C 6 , 15 N 4 ) respectively) were added in constant amount before
Jesper Krogh, Peter Plomgaard, Ruth Frikke-Schmidt, Sten Velschow, Jesper Johannesen, Linda Maria Hilsted, Malene Schrøder, and Ulla Feldt-Rasmussen
-0564) using a Wallac DBS Puncher (PerkinElme). Cortisol was extracted from the DBS with 200 μL extraction solvent (acetonitrile/water: 80:20) containing the stable isotopically labeled cortisol-( 13 C3) internal standard (30 nmol/L) by vigorously mixing
Kathrin R Frey, Tina Kienitz, Julia Schulz, Manfred Ventz, Kathrin Zopf, and Marcus Quinkler
Winterer JC Loriaux DL . Daily cortisol production rate in man determuned by stable isotope dilution/mass spectrometry . Journal of Clinical Endocrinology and Metabolism 1991 72 39 – 45 . ( https://doi.org/10.1210/jcem-72-1-39 ) 10.1210/jcem-72
María L Bacigalupo, Verónica G Piazza, Nadia S Cicconi, Pablo Carabias, Andrzej Bartke, Yimin Fang, Ana I Sotelo, Gabriel A Rabinovich, María F Troncoso, and Johanna G Miquet
Stable Isotope Labeling by amino Acids in Cell Culture (SILAC) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) quantitative proteomic analysis identifies galectin-1 as a potential biomarker for predicting sorafenib resistance in liver
Siphiwe N Dlamini, Zané Lombard, Lisa K Micklesfield, Nigel Crowther, Shane A Norris, Tracy Snyman, Andrew A Crawford, Brian R Walker, and Julia H Goedecke
Extraction of steroids from serum All calibrators, internal standards (labelled with stable isotope deuterium; corticoseterone-d8 and cortisol-d4), and controls were purchased from Chromsystems (Grafelfing, Germany). A liquid–liquid extraction technique
J A Tamblyn, C Jenkinson, D P Larner, M Hewison, and M D Kilby
.01.049 ) 10.1016/j.jchromb.2016.01.049 40 Ogawa S Ooki S Shinoda K Higashi T. Analysis of urinary vitamin D(3) metabolites by liquid chromatography/tandem mass spectrometry with ESI-enhancing and stable isotope-coded derivatization . Analytical and
Teresa Lam, Mark McLean, Amy Hayden, Anne Poljak, Birinder Cheema, Howard Gurney, Glenn Stone, Neha Bahl, Navneeta Reddy, Haleh Shahidipour, and Vita Birzniece
, coefficients of variation (CVs) for LRa and Lox are 3.5 and 6.1% respectively ( 18 ). Hepatic urea production The rate of urea production was measured by the urea turnover technique using stable isotope methodology with 15 N 2 -Urea as tracer, as
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