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Huiyuan Zhai Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Dongxu Wang Department of Geriatrics, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Yong Wang Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Hongwei Gu Central Laboratory, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Juan Jv Department of Cardiology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Liangliang Yuan Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Chao Wang Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Leiyao Chen Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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macrophage mRNA expression of TNF-α and IL-18 was significantly upregulated, while M2 macrophage mRNA expression of Arg1 and IL-10 was downregulated in db/db mice. Kaempferol administration effectively reversed the M1-specific macrophage and M2-specific

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0379
Volume/Issue:
Volume 13: Issue 5
Open access
Xianghe Chen College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Xinyu Zeng College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Xiao Qiu College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Chi Liu College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Pengcheng Lu College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Ziming Shen College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Xiangxiang Zhou College of Physical Education, Yangzhou University, Yangzhou, Jiangsu, China

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Kang Yang Department of Rehabilitation Medicine, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China

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concentration and purity of RNA were detected, and the extracted RNA was reverse transcribed into cDNA by Takara reverse transcription kit (RR037A, Takara Bio). Then, the mRNA expression was quantitatively detected using the Takara quantitative kit (RR014A

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0448
Volume/Issue:
Volume 13: Issue 3
Open access
Katrine M Lauritsen Steno Diabetes Center Aarhus, Aarhus, Denmark
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
Danish Diabetes Academy, Odense University Hospital, Odense, Denmark

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Jens Hohwü Voigt Steno Diabetes Center Aarhus, Aarhus, Denmark

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Steen Bønløkke Pedersen Steno Diabetes Center Aarhus, Aarhus, Denmark
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark

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Troels K Hansen Steno Diabetes Center Aarhus, Aarhus, Denmark

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Niels Møller Steno Diabetes Center Aarhus, Aarhus, Denmark
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark

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Niels Jessen Steno Diabetes Center Aarhus, Aarhus, Denmark
Department of Biomedicine, Aarhus University, Aarhus, Denmark

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Lars C Gormsen Department of Nuclear Medicine and PET Centre, Aarhus University Hospital, Aarhus, Denmark

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Esben Søndergaard Steno Diabetes Center Aarhus, Aarhus, Denmark
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
Danish Diabetes Academy, Odense University Hospital, Odense, Denmark

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LightCycler SYBR Green master mix. mRNA levels of GLUT4, G0S2, CIDEC, PDE3B, ATGL, HSL, PLIN1, ANGPTL4, LPL, IL6, PTEN, PGC1A and PIK3R1 were analyzed. Beta 2 microglobulin was used as the housekeeping gene and β2 microglobulin mRNA levels were similar in both

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Research Article
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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-21-0558
Volume/Issue:
Volume 11: Issue 4
Open access
Yang Yu Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Hairong Hao Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Linghui Kong Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Jie Zhang Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Feng Bai Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Fei Guo Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Pan Wei Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Rui Chen Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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Wen Hu Department of Endocrinology and Metabolism, Huai’an Hospital Affiliated to Xuzhou Medical University and Huai’an Second People’s Hospital, Huai’an, Jiangsu, China

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mainly in muscle tissue. Therefore, the expression of BCAAs-metabolizing enzymes in rat skeletal hindlimb muscles was investigated. Branched-chain α-keto acid dehydrogenase (BCKDH) and ACAD are the two rate-limiting enzymes of BCAA catabolism. The mRNA

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0079
Volume/Issue:
Volume 12: Issue 10
Open access
Jing Li School of Physical Education, Liaoning Normal University, Dalian, Liaoning, China

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Xuejie Yi Exercise and Health Research Center, Department of Kinesiology, Laboratory Management Center, Shenyang Sport University, Shenyang, Liaoning, China

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Tao Li Exercise and Health Research Center, Department of Kinesiology, Laboratory Management Center, Shenyang Sport University, Shenyang, Liaoning, China

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Tingting Yao School of Physical Education, Liaoning Normal University, Dalian, Liaoning, China

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Dongyang Li Exercise and Health Research Center, Department of Kinesiology, Laboratory Management Center, Shenyang Sport University, Shenyang, Liaoning, China

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Guangxuan Hu Exercise and Health Research Center, Department of Kinesiology, Laboratory Management Center, Shenyang Sport University, Shenyang, Liaoning, China

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Yongqi Ma Exercise and Health Research Center, Department of Kinesiology, Laboratory Management Center, Shenyang Sport University, Shenyang, Liaoning, China

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Bo Chang Exercise and Health Research Center, Department of Kinesiology, Laboratory Management Center, Shenyang Sport University, Shenyang, Liaoning, China

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Shicheng Cao Department of Sports Medicine, School of Public and Basic Sciences, China Medical University, Shenyang, Liaoning, China

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(Applied Biosystems ). Thereafter, an RT-PCR kit (Promega) was used to determine the target mRNA content using an RT-PCR system (Applied Biosystems), according to the manufacturer’s instructions. The primers used in this study were as follows: Pgc1a , 5

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-21-0625
Volume/Issue:
Volume 11: Issue 3
Open access
Zhandong Lei Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
Department of Anatomy, Shanxi Medical University, Taiyuan, China

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Yunfei Chen Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Jin Wang Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Yan Zhang Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Wenjuan Shi Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Xuejiao Wang Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Dehai Xing Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Dongxue Li Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Xiangying Jiao Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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mice (KO, catalog number: ENSMUST00000074519.12) were originally generated by GemPharmatech Co., Ltd (Nanjing, China) using CRISPR/Cas9 technology. In brief, Cas9 mRNA and gRNA (sequence, S1: 5’-ACACGGTGTGCTCCTAGCG-3’; S2: 5’-CAACCAATCAGCGAGGCCGC-3’; S3

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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-21-0641
Volume/Issue:
Volume 11: Issue 4
Open access
Wenjun Long Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Tuo Zhou Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Xiuping Xuan Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Qiuli Cao Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Zuojie Luo Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Yingfen Qin Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Qin Ning Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Xiaoping Luo Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Xuemei Xie Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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. Insulin signaling was impaired in skeletal muscle of male CG-IUGR To investigate the insulin signaling in skeletal muscle of male CG-IUGR, the protein and mRNA level of insulin receptor (IR)-β and insulin receptor substrate (IRS)-1, two canonical

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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-21-0203
Volume/Issue:
Volume 11: Issue 1
Open access
Yi Jia School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Yanan Yang School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Jing Qu School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Lijun Yin School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Xiaohui Wang School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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) and chemerin (−/−) mice at DNA level. The mRNA levels of chemerin in (C) gastrocnemius and (D) liver of WT and chemerin (−/−) mice. (E) The protein levels of chemerin in multiply organs of WT and chemerin (−/−) mice. eWAT, epididymal white adipose

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Research Article
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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0484
Volume/Issue:
Volume 13: Issue 4
Open access
Chenmin Wei Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
Department of Endocrinology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Zichen Zhang Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Qi Fu Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Yunqiang He Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Tao Yang Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Min Sun Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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protein expression levels ( Fig. 2C , D and E ). Figure 2 Effects of palmitate and oleate on mRNA and total protein expressions of K ATP channels. Cells were exposed to 0.5 mM PA or OA for 48 h and then washed out for 24 h with vehicle. qRT

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Research Article
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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0221
Volume/Issue:
Volume 12: Issue 1
Open access
Ying Pei Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Rui Wang School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Wanyu Chen Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Shulin Yi Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Chen Huang Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Shaochan Liang School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Hongying Cao School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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Yifei Xu Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, China

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Bo Tan Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China

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, HFD, and HGD groups ( Fig. 4C , D and E , P < 0.05). These data suggested that inhibitory neurotransmitters (NO) played a critical role in colonic neuromuscular relaxation in the HGD group. Next, the mRNA and protein expression levels of nNOS were

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0078
Volume/Issue:
Volume 12: Issue 9
Open access