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Universidade de São Paulo, Zebrafish Facility, São Paulo, São Paulo, Brazil
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insertions, deletions, and amino acid changes, indicating the efficiency of the technique. The phenotypic analyses were carried out by observing the animals under a magnifying glass (Nikon), and the animals presented several anomalies such as absence of
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). GDH is highly expressed in pancreatic β-cells, liver, kidney and brain, where it plays an important role in the metabolism of amino acids and ammonia ( 3 ). In pancreatic β-cells, it catalyses the oxidative deamination of glutamate to alpha
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16-year-old girl with primary amenorrhoea and poor breast development due to isolated FSH deficiency. She was found to have a homozygous nonsense mutation in amino acid position 76 of the FSH β-subunit gene. The induction of fertility using