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Chenmin Wei Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
Department of Endocrinology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Zichen Zhang Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Qi Fu Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Yunqiang He Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Tao Yang Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Min Sun Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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-rabbit IgG (H+L), Cat# 34206ES60) and Alexa Fluor 594 (donkey anti-mouse IgG (H+L), Cat# 34112ES60) were purchased from Yeasen Biotech Co (Shanghai, China). Cell culture, FFA preparation, and cell treatment MIN6 cells (passage 20–45) were routinely

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0221
Volume/Issue:
Volume 12: Issue 1
Open access
Yi Jia School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Yanan Yang School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Jing Qu School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Lijun Yin School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Xiaohui Wang School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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:1000,2438S, Cell Signaling Technology) primary antibodies. The membranes were incubated at room temperature for 1 h with the secondary antibodies (HRP-labeled goat anti-mouse IgG(H+L),1:1000, A0216, Beyotime; goat anti-rabbit IgG(H+L)-HRP antibody, 1

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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0484
Volume/Issue:
Volume 13: Issue 4
Open access
Li Qian Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Yuxiao Zhu Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Yan Luo Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Mu Zhang Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Liping Yu Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, Aurora, Colorado, USA

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Yu Liu Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Tao Yang Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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-risk autoantibodies and greatly improve sensitivity and disease specificity. Moreover, ECL assays, which capture all of the immunoglobulin classes (IgG, IgM, IgA, and IgE), are more sensitive than ELISA, which captures only IgG. More importantly, previous studies have

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0325
Volume/Issue:
Volume 11: Issue 12
Open access
Shaomin Shi Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, China

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Xinghua Chen Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, China

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Wen Yu Department of Immunology, School of Medicine, Yangtze University, Jingzhou, China

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Xiaolan Ke Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, China

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Tean Ma Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, China

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, Abcam), NPHS2 (1:2000, ab181143, Abcam), GLP-1R (1:250, ab218532, Abcam), overnight at 4°C, HRP-labeled goat anti-rabbit secondary antibody immunoglobulin G (IgG) (1:50, A0208, Beyotime, Shanghai, China) was incubated at room temperature for 1 h, DAB

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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0284
Volume/Issue:
Volume 12: Issue 10
Open access
Huiyuan Zhai Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Dongxu Wang Department of Geriatrics, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Yong Wang Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Hongwei Gu Central Laboratory, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Juan Jv Department of Cardiology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Liangliang Yuan Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Chao Wang Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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Leiyao Chen Department of Pharmacy, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Affiliated with Nanjing University of Chinese Medicine, Nanjing, China

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overnight. Following washing, the sections were incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (cat. Ab6721, Abcam) at a dilution of 1:1000 for 1 h. The F4/80 signal was detected using the DAB method. Finally

Article Type:
Research Article
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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0379
Volume/Issue:
Volume 13: Issue 5
Open access
Fabyan Esberard de Lima Beltrão Lauro Wanderley University Hospital, Federal University of Paraíba, João Pessoa, Paraíba, Brazil
Postgraduate Program in Nutritional Sciences, Department of Nutrition, Center for Health Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil
University Centre of João Pessoa (UNIPE), João Pessoa, Paraíba, Brazil

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Daniele Carvalhal de Almeida Beltrão University Centre of João Pessoa (UNIPE), João Pessoa, Paraíba, Brazil
Postgraduate Program in Cognitive Neuroscience and Behavior, Center for Health Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil

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Giulia Carvalhal Center for Biological and Health Sciences, Federal University of Campina Grande, Campina Grande, Paraíba, Brazil

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Fabyo Napoleão de Lima Beltrão Department of Medicine, Faculty of Medical Sciences, João Pessoa, Brazil

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Igor Motta de Aquino Metropolitan Hospital Dom José Maria Pires, Santa Rita, Paraíba, Brazil

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Thaíse da Silva Brito New Hope Medical School – FAMENE, João Pessoa, Paraíba, Brazil

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Barbara Costa Paulino Postgraduate Program in Nutritional Sciences, Department of Nutrition, Center for Health Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil

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Elisa Aires Postgraduate Program in Interactive Processes of Organs and Systems, Health & Science Institute, Federal University of Bahia, Salvador, Bahia, Brazil

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Diana Viegas Internal Medicine Department, rede UniFTC, Salvador, Bahia, Brazil

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Fabio Hecht The Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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Bruno Halpern Weight Control Centre, Hospital 9 de Julho, São Paulo, São Paulo, Brazil

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Liana Clebia De Morais Pordeus Postgraduate Program in Cognitive Neuroscience and Behavior, Center for Health Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil

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Maria da Conceição Rodrigues Gonçalves Postgraduate Program in Nutritional Sciences, Department of Nutrition, Center for Health Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil

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Helton Estrela Ramos Postgraduate Program in Interactive Processes of Organs and Systems, Health & Science Institute, Federal University of Bahia, Salvador, Bahia, Brazil
Department of Biorregulation, Health Sciences Institute, Federal University of Bahia, Bahia, Brazil
Postgraduate Program in Medicine and Health, Medical School of Medicine, Federal University of Bahia, Salvador, Bahia, Brazil

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All patients tested positive for SARS-CoV-2 using the real-time quantitative reverse-transcriptase-PCR (rRT-qPCR) with samples from the respiratory tract and, in cases of negative rRT-qPCR, using clinical, radiological, and serological (IgG positive

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Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0290
Volume/Issue:
Volume 11: Issue 10
Open access
Wenjun Long Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Tuo Zhou Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Xiuping Xuan Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Qiuli Cao Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Zuojie Luo Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Yingfen Qin Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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Qin Ning Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Xiaoping Luo Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Xuemei Xie Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China

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probed with secondary horseradish-peroxidase-conjugated IgG (1:10,000 dilution; Aspen, Wuhan, China) for 2 h at room temperature. The membranes were washed with ECL solution for 5 min and repeated for five times, followed by chemiluminescence detection in

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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-21-0203
Volume/Issue:
Volume 11: Issue 1
Open access
Zeting Li Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Ling Pei Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Huangmeng Xiao Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Nan Chen Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Fenghua Lai Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Shufang Yue Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Changliu Xu Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Yanbing Li Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Haipeng Xiao Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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Xiaopei Cao Department of Endocrinology, Sun Yat-sen University First Affiliated Hospital, Guangzhou, China

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temperature. Primary GLP-1 antibodies (1:100, ab22625, Abcam) were diluted in the blocking solution, and the cells were incubated with the antibodies at room temperature for 2 h. Subsequently, Alexa Fluor 488 goat anti-mouse IgG (H&L) (1:1000, ab150113, Abcam

Article Type:
Research Article
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Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0548
Volume/Issue:
Volume 13: Issue 11
Open access