Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands
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Department of Pediatric Neuro-Oncology, Prinses Máxima Centrum, Utrecht, The Netherlands
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Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands
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rabbit monoclonal GR antibody (1:500, D6H2L, Cell Signalling technology) and mouse monoclonal MR antibody (1:500, MR1-18 1D5 and MR 4G5) ( 20 ) in 1% BSA/PBST. After washing, sections were incubated with AlexaFluor-488 labeled goat-anti-rabbit IgG and
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solution (BD Biosciences). To differentiate monocytes from other leukocytes, anti-human CD14+ IgG conjugated with FITC (ab28061, Abcam) was first added and incubated on ice for 30 min followed by either surface LDLR or intracellular IDOL antibodies staining