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T L C Wolters Division of Endocrinology, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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C D C C van der Heijden Division of Experimental Internal Medicine, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands
Division of Vascular Medicine, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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N van Leeuwen Department of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands

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B T P Hijmans-Kersten Department of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands

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M G Netea Division of Experimental Internal Medicine, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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J W A Smit Division of Endocrinology, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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D H J Thijssen Department of Physiology, Radboud Institute for Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands
Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UK

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A R M M Hermus Division of Endocrinology, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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N P Riksen Division of Vascular Medicine, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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R T Netea-Maier Division of Endocrinology, Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands

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determined with ELISA. For E-Selectin, MMP2, VCAM1, hsCRP, and IL18, DuoSet ELISA (R&D Systems, Abingdon, United Kingdom) was used with a sensitivity of 93.8 pg/mL for E-Selectin, 625 pg/mL for MMP2, 15.6 pg/mL for VCAM1 and hsCRP, and 11.7 pg/mL for IL18

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0430
Volume/Issue:
Volume 8: Issue 12
Open access
Jana Ernst Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse, Halle (Saale), Germany

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Katharina Gert Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse, Halle (Saale), Germany

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Frank Bernhard Kraus Central Laboratory, University Hospital Halle (Saale), Ernst-Grube-Strasse, Halle (Saale), Germany

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Ulrike Elisabeth Rolle-Kampczyk Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany

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Martin Wabitsch Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany

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Faramarz Dehghani Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse, Halle (Saale), Germany

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Kristina Schaedlich Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse, Halle (Saale), Germany

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-sensitive leptin ELISA, IBL, Hamburg, Germany) and adiponectin (Quantikine® ELISA Human Total Adiponectin/Acrp30, BioVendor, Kassel, Germany) were measured by ELISA according to manufacturer’s manual. ELISA data was normalized to the total protein concentration of

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0549
Volume/Issue:
Volume 9: Issue 7
Open access
Ming Zhu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Bingxin Xu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Meng Wang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Shangyun Liu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Yue Zhang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Chao Zhang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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plate reader. Cell surface ELISA HEK293T cells were seeded onto poly- l -lysine-coated 12-well plates at 10 5 cells/well and transfected with indicated plasmid the next day. After 24 h of transfection, cells were washed with D-PBS three times

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0019
Volume/Issue:
Volume 8: Issue 4
Open access
Charlotte Höybye Department of Endocrinology, Metabolism and Diabetology, Karolinska University Hospital and Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden

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Andreas F H Pfeiffer Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Klinik für Endokrinologie & Stoffwechselmedizin, Berlin, Germany

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Diego Ferone IRCCS AOU San Martino-IST, Università di Genova – Endocrinologia DiMI, Dipartimento di Medicina Interna e Specialità Mediche, & CEBR, Centro di Eccellenza per la Ricerca Biomedica, Genova, Italy

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Jens Sandahl Christiansen Medicinsk Endokrinologist Afd., MEA, NBG, Århus Sygehus, Århus, Denmark

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David Gilfoyle Ascendis Pharma A/S, Hellerup, Denmark

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Eva Dam Christoffersen Ascendis Pharma A/S, Hellerup, Denmark

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Eva Mortensen Ascendis Pharma Inc., Palo Alto, California, USA

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Jonathan A Leff Ascendis Pharma Inc., Palo Alto, California, USA

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Michael Beckert Ascendis Pharma A/S, Hellerup, Denmark

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, immunogenicity evaluation and clinical laboratory testing. Serum for anti-GH-binding antibodies was drawn at screening and Days 0, 8, 22, 29 and 42 and analyzed using a validated ELISA (Millipore BioPharma Services, currently known as Eurofins Pharma Bioanalysis

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0007
Volume/Issue:
Volume 6: Issue 3
Open access
Henrik H Thomsen Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Holger J Møller Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Christian Trolle Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Kristian A Groth Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Anne Skakkebæk Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Anders Bojesen Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Christian Høst Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Claus H Gravholt Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark
Medical Research Laboratories, Departments of Clinical Biochemistry, Molecular Medicine, Department of Clinical Genetics, Department of Endocrinology and Internal Medicine, Clinical Institute, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark

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Committee (no. 1996/3561). Assays The plasma concentration of sCD163 was determined in duplicate in samples that had been frozen at −20 °C by an in-house sandwich ELISA using a BEP-2000 ELISA-analyzer (Dade Behring, Deerfield, IL, USA) essentially as

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-13-0067
Volume/Issue:
Volume 2: Issue 4
Open access
Rui Zhang Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Xinmei Huang Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Yue Li Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Zhiyan Yu Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Yueyue Wu Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Bingbing Zha Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Heyuan Ding Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Shufei Zang Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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Jun Liu Department of Endocrinology, Shanghai Fifth People’s Hospital affiliated to Fudan University, Minhang District, Shanghai, People’s Republic of China

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glucose levels. After a 3-day recovery period, all rats were euthanized using pentobarbital sodium. The serum insulin levels of the rats were quantified using rat ELISA kits (TSZ, San Francisco, CA, USA). A homeostasis model assessment (HOMA) method refers

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0316
Volume/Issue:
Volume 10: Issue 12
Open access
Letícia Ribeiro Oliveira Interdisciplinary Group for Studies of Sex Determination and Differentiation (GIEDDS), School of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil
Department of Pediatrics, Federal University of Uberlandia (UFU), Uberlandia, Minas Gerais, Brazil

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Carlos Alberto Longui Pediatric Endocrinology Unit, School of Medical Sciences, Irmandade da Santa Casa de Misericordia de Sao Paulo, Sao Paulo, Brazil

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Guilherme Guaragna-Filho Interdisciplinary Group for Studies of Sex Determination and Differentiation (GIEDDS), School of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil
Department of Pediatrics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil

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José Luiz Costa School of Pharmaceutical Sciences, UNICAMP, Campinas, Sao Paulo, Brazil
Poison Control Center, FCM, UNICAMP, Campinas, Sao Paulo, Brazil

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Rafael Lanaro Poison Control Center, FCM, UNICAMP, Campinas, Sao Paulo, Brazil

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David Antônio Silva Laboratory of Physiology, Division of Clinical Pathology, Clinical Hospital, UNICAMP, Campinas, Sao Paulo, Brazil

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Maria Izabel Chiamolera Fleury Group, Sao Paulo, Brazil

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Maricilda Palandi de Mello Interdisciplinary Group for Studies of Sex Determination and Differentiation (GIEDDS), School of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil
Laboratory of Human Molecular Genetics, Center for Molecular Biology and Genetics Engineering (CBMEG), UNICAMP, Campinas, Sao Paulo, Brazil

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André Moreno Morcillo Department of Pediatrics, FCM, UNICAMP, Campinas, Sao Paulo, Brazil

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Andrea Trevas Maciel-Guerra Interdisciplinary Group for Studies of Sex Determination and Differentiation (GIEDDS), School of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil
Department of Medical Genetics and Genomic Medicine, FCM, UNICAMP, Campinas, Sao Paulo, Brazil

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Gil Guerra-Junior Interdisciplinary Group for Studies of Sex Determination and Differentiation (GIEDDS), School of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil
Department of Pediatrics, FCM, UNICAMP, Campinas, Sao Paulo, Brazil

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-MS/MS at both time of the study. Concerning IA methods, total testosterone was assessed by electrochemiluminescence (ECLIA) (Roche, Germany, Cobas E, 05200067190, detection limit: 0.03–15 ng/mL); DHT by enzyme immunoassay (ELISA) (DBC – Diagnostic Biochem

Article Type:
Research Article
Collections:
Paediatric Endocrinology Collection
DOI:
https://doi.org/10.1530/EC-20-0454
Volume/Issue:
Volume 9: Issue 11
Open access
Agnieszka Adamska Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Białystok, Białystok, Poland

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Aleksandra Maria Polak Department of Internal Medicine and Metabolic Diseases, Medical University of Białystok, Białystok, Poland

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Anna Krentowska Department of Internal Medicine and Metabolic Diseases, Medical University of Białystok, Białystok, Poland

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Agnieszka Łebkowska Department of Internal Medicine and Metabolic Diseases, Medical University of Białystok, Białystok, Poland

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Justyna Hryniewicka Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Białystok, Białystok, Poland

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Monika Leśniewska Department of Reproduction and Gynecological Endocrinology, Medical University of Białystok, Białystok, Poland

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Irina Kowalska Department of Internal Medicine and Metabolic Diseases, Medical University of Białystok, Białystok, Poland

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assessed with colorimetric method (Cobas c111, Roche Diagnostic). Serum concentration of fetuin-B was estimated with ELISA Kit (Cloud Clone, Wuhan, China), following the manufacturer’s protocol. The degree of precision of the ELISA system in terms of

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0243
Volume/Issue:
Volume 8: Issue 8
Open access
Janko Sattler Adrenal Steroid Group, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Department of Rheumatology and Clinical Immunology, Charité-University Medicine, Berlin, Germany

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Jinwen Tu Adrenal Steroid Group, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Concord Clinical School, The University of Sydney, Sydney, Australia

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Shihani Stoner Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia

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Jingbao Li Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University, Shaanxi, China

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Frank Buttgereit Department of Rheumatology and Clinical Immunology, Charité-University Medicine, Berlin, Germany

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Markus J Seibel Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Concord Clinical School, The University of Sydney, Sydney, Australia
Department of Endocrinology & Metabolism, Concord Hospital, Sydney, Australia

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Hong Zhou Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Concord Clinical School, The University of Sydney, Sydney, Australia

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Mark S Cooper Adrenal Steroid Group, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Concord Clinical School, The University of Sydney, Sydney, Australia
Department of Endocrinology & Metabolism, Concord Hospital, Sydney, Australia

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transparent supernatant was then transferred to a fresh microcentrifuge tube as serum. Levels of corticosterone and adrenocorticotropic hormone (ACTH) were determined with commercial enzyme immunoassay kits (Mouse ACTH ELISA, Sigma Aldrich; Corticosterone EIA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0361
Volume/Issue:
Volume 7: Issue 2
Open access
Marloes Emous Centre for Obesity Northern-Netherlands (CON), Medical Centre Leeuwarden, Leeuwarden, The Netherlands

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Merel van den Broek Centre for Obesity Northern-Netherlands (CON), Medical Centre Leeuwarden, Leeuwarden, The Netherlands

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Ragnhild B Wijma Centre for Obesity Northern-Netherlands (CON), Medical Centre Leeuwarden, Leeuwarden, The Netherlands

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Loek J M de Heide Centre for Obesity Northern-Netherlands (CON), Medical Centre Leeuwarden, Leeuwarden, The Netherlands

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Gertjan van Dijk GELIFES-Neurobiology, Department of Behavioral Neuroscience, University of Groningen, Groningen, The Netherlands

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Anke Laskewitz Certe Laboratories, Medical Center Leeuwarden, Leeuwarden, The Netherlands

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Erik Totté Centre for Obesity Northern-Netherlands (CON), Medical Centre Leeuwarden, Leeuwarden, The Netherlands

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Bruce H R Wolffenbuttel Department of Endocrinology, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands

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André P van Beek Centre for Obesity Northern-Netherlands (CON), Medical Centre Leeuwarden, Leeuwarden, The Netherlands
Department of Endocrinology, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands

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GLP-1 and PYY were determined using commercial ELISA kits on a two-plate ELISA processing system (DS2, DYNEX Technologies, Chantilly, VA, USA), as per the manufacturer’s instructions. The following commercial ELISA kits were used for the active form

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0268
Volume/Issue:
Volume 8: Issue 7
Open access