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, Abcam), NPHS2 (1:2000, ab181143, Abcam), GLP-1R (1:250, ab218532, Abcam), overnight at 4°C, HRP-labeled goat anti-rabbit secondary antibody immunoglobulin G (IgG) (1:50, A0208, Beyotime, Shanghai, China) was incubated at room temperature for 1 h, DAB
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. Diabetes insipidus (DI) was seen in 46.6% of patients. MRI showed pituitary enlargement and thickened stalk in 81.8 and 90.6% of patients, respectively. The most common histopathological subtypes were lymphocytic ( n = 35) followed by IgG4 ( n = 9
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overnight. Following washing, the sections were incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (cat. Ab6721, Abcam) at a dilution of 1:1000 for 1 h. The F4/80 signal was detected using the DAB method. Finally
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(Invitrogen) for permeabilized conditions with 1 μL anti-NIS (Proteintech, Wuhan, China). After washing, cells were incubated with donkey anti-rabbit IgG H&L (Abcam). The fluorescence of 10 7 cells per tube was assayed using the fluorescence-activated cell
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general procedure was followed but antigen retrieval was performed using 10 mM citric acid (pH 6.0). Following staining with primary antibody, samples were incubated with biotinylated anti-rabbit or anti-mouse IgG secondary antibody, followed by peroxidase
Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia
Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
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Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
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ten top ranked genes present in the obesity signature, the majority were linked to inflammation (Supplementary Table 2), including Fc fragment of IgG-binding protein ( FCGBP ), lysozyme ( LYZ ), chemokine ligand motif 10 ( CXCL10 ), myeloid cell
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-buffered saline, 0.1% Tween 20, 5% nonfat dry milk) for 1 h and incubated overnight at 4°C with the primary antibody. Blots were developed using a peroxidase-conjugated anti-rabbit and anti-mouse IgG and a chemiluminescent detection system (Santa Cruz
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have a marked defect in B-cell proliferation ( 24 , 48 ). SAP could form a ternary complex with the Lyn kinase and the inhibitory IgG Fc receptor FCGR2B to regulate B-cell proliferation and survival, leading to a decrease in B-cell proliferation and an
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washed, treated with 5% bovine serum albumin for 30 min and subsequently incubated overnight with rabbit anti-Hepcidin (1:100; Santa Cruz Biotechnology) at 4°C. The sections were rinsed and then incubated with biotinylated goat anti-rabbit IgG (1:200) for
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), followed by a goat anti-rabbit IgG-HRP (1:10,000 dilution, Santa Cruz Biotechnology) secondary antibody. Immunoreactivity was detected using chemiluminescence as recommended by the manufacturer (Pierce Biotechnology). In order to obtain a relative