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Anna Olsson-Brown Department of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool, UK
The Clatterbridge Cancer Centre, Wirral, UK

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Rosemary Lord The Clatterbridge Cancer Centre, Wirral, UK

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Joseph Sacco The Clatterbridge Cancer Centre, Wirral, UK
Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool, UK

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Jonathan Wagg Roche Innovation Center, Basel, Switzerland

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Mark Coles Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK

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Munir Pirmohamed Department of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool, UK

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approached to donate a blood sample in line with the Clatterbridge Biobank ethical approval. Informed consent was obtained. The presence of anti-Tg antibodies was analysed by ELISA using a human anti-thyroglobulin ELISA kit (ab178631) on serum isolated from

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0473
Volume/Issue:
Volume 9: Issue 4
Open access
Lauren Bell Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK
Endocrinology & Diabetes, Salford Royal NHS Foundation Trust, Salford, UK

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Ann Louise Hunter Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK
Endocrinology & Diabetes, Salford Royal NHS Foundation Trust, Salford, UK

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Angelos Kyriacou Endocrinology & Diabetes, Salford Royal NHS Foundation Trust, Salford, UK
CEDM Centre of Endocrinology, Diabetes & Metabolism, Limassol, Cyprus

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Annice Mukherjee Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK
Endocrinology & Diabetes, Salford Royal NHS Foundation Trust, Salford, UK

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Akheel A Syed Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK
Endocrinology & Diabetes, Salford Royal NHS Foundation Trust, Salford, UK

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classification. TRAb assay The TRAb assay used in the study period was a commercial third-generation TSH receptor autoantibody enzyme linked immunosorbent assay (ELISA) kit supplied by Thermo Scientific B.R.A.H.M.S (Hennigsdorf, Germany) and

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0082
Volume/Issue:
Volume 7: Issue 4
Open access
Hathairat Rueangdetnarong Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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Rattanaporn Sekararithi Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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Thidarat Jaiwongkam Cardiac Electrophysiology Research and Training Center (CERT), Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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Sirinart Kumfu Cardiac Electrophysiology Research and Training Center (CERT), Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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Nipon Chattipakorn Cardiac Electrophysiology Research and Training Center (CERT), Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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Theera Tongsong Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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Phudit Jatavan Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

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-alpha The TNF-α ELISA (Invitrogen) is used for the quantitative determination of Hu TNF-α in human. The standard Hu TNF-α is prepared by following the protocol of ELISA kit. Determine the number of eight well strips needed for the assay. Insert these strips

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0093
Volume/Issue:
Volume 7: Issue 5
Open access
Rachel Forfar Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Mashal Hussain Centre for Endocrinology, William Harvey Research Institute, Queen Mary University of London, London, UK

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Puneet Khurana Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Jennifer Cook Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Steve Lewis Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Dillon Popat Centre for Endocrinology, William Harvey Research Institute, Queen Mary University of London, London, UK

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David Jackson Centre for Endocrinology, William Harvey Research Institute, Queen Mary University of London, London, UK

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Ed McIver Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Jeff Jerman Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Debra Taylor Centre for Therapeutics Discovery, LifeArc, Accelerator Building, Open Innovation Campus, Stevenage, UK

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Adrian JL Clark Centre for Endocrinology, William Harvey Research Institute, Queen Mary University of London, London, UK

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Li F Chan Centre for Endocrinology, William Harvey Research Institute, Queen Mary University of London, London, UK

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1-24 was purchased from Cambridge Research Biochemicals (Cleveland, UK). Homogeneous time-resolved fluorescence (HTRF) cAMP dynamic 2 assay kits were from Cisbio (Codolet, France). Progesterone ELISA kit was purchased from Cayman Chemical. GeneBLAzer

Article Type:
Research Article
Collections:
Adrenal
DOI:
https://doi.org/10.1530/EC-22-0338
Volume/Issue:
Volume 11: Issue 12
Open access
Xiangyu Gao Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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Wanwan Sun Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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Yi Wang Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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Yawen Zhang Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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Rumei Li Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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Jinya Huang Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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Yehong Yang Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

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-linked immunesorbent assay (ELISA) in the Department of Clinical Laboratory in Huashan Hospital (Shanghai, China). All samples were measured in duplicate. GADA was detected by ELISA kit (Euroimmun AG, Lübeck, Germany) according to the instruction of the manufacturer

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0379
Volume/Issue:
Volume 8: Issue 11
Open access
Göran Oleröd Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden

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Lillemor Mattsson Hultén Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden

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Ola Hammarsten Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden

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Eva Klingberg Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden

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quantification (LoQ) was 12.5 nmol/L at a CV of 8%. The free 25(OH)D concentration in serum was measured with enzyme-linked immunosorbent assay (ELISA) using a commercial kit (Future Diagnostics Solutions, Wijchen, The Netherlands), hereafter, named directly

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-16-0078
Volume/Issue:
Volume 6: Issue 2
Open access
Jana Ernst Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Urszula Grabiec Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Kathrin Falk Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Faramarz Dehghani Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Kristina Schaedlich Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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concentrations of leptin (high sensitive Leptin ELISA, IBL, Hamburg, Germany) and adiponectin (Quantikine® ELISA Human Total Adiponectin/Acrp30, BioVendor, Kassel, Germany) by ELISA according to manufacturer’s manual. ELISA data were normalized to the protein

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0548
Volume/Issue:
Volume 9: Issue 2
Open access
Giovanni Fanni Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden

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Petros Katsogiannos Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden

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Bipasha Nandi Jui Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden

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Magnus Sundbom Department of Surgical Sciences, Uppsala University, Uppsala, Sweden

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Susanne Hetty Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden

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Maria J Pereira Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden

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Jan W Eriksson Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden

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immediately performed, were frozen and stored at −80°C. For analyses, commercially available ELISA or multiplex kits were used: glucagon and glicentin (Mercodia, Uppsala, Sweden); total glucagon-like peptide (GLP)-1 (7-36 and 9-36) and total glucose

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0172
Volume/Issue:
Volume 11: Issue 8
Open access
Mette Bøgehave Department of Clinical Biochemistry, Hospital South West Jutland, University Hospital of Southern Denmark, Esbjerg, Denmark
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Denmark

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Dorte Glintborg Department of Endocrinology, Odense University Hospital, Odense, Denmark
Department of Clinical Research, University of Southern Denmark, Odense, Denmark
OPEN, Open Patient data Explorative Network, Odense University Hospital, Region of Southern Denmark, Odense, Denmark

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Jørgen Brodersen Gram Department of Clinical Biochemistry, Hospital South West Jutland, University Hospital of Southern Denmark, Esbjerg, Denmark
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Denmark

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Else-Marie Bladbjerg Department of Clinical Biochemistry, Hospital South West Jutland, University Hospital of Southern Denmark, Esbjerg, Denmark
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Denmark

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Marianne Skovsager Andersen Department of Endocrinology, Odense University Hospital, Odense, Denmark
Department of Clinical Research, University of Southern Denmark, Odense, Denmark

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Johannes Jakobsen Sidelmann Department of Clinical Biochemistry, Hospital South West Jutland, University Hospital of Southern Denmark, Esbjerg, Denmark
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Denmark

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/L × min), representing the total amount of kallikrein formed. Coagulation factors and inhibitors The plasma protein concentration of coagulation factor XII (FXII) was determined with enzyme-linked immunosorbent assay (ELISA), as described

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-22-0455
Volume/Issue:
Volume 12: Issue 4
Open access
Kim Magaly Pabst Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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Robert Seifert Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
Department of Nuclear Medicine, University Hospital Münster, Münster, Germany

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Nader Hirmas Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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Martina Broecker-Preuss Department of Medicine, Ruhr-University Bochum, University Hospital, Knappschaftskrankenhaus Bochum, Bochum, Germany

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Manuel Weber Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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Wolfgang Peter Fendler Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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Timo Bartel Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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Sarah Theurer German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany
Institute of Pathology, University Hospital Essen, University Duisburg-Essen, Essen, Germany

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Ken Herrmann Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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Rainer Görges Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, Essen, Germany
German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany

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enzyme-linked immunosorbent assay (ELISA) with an LoQ in our laboratory of 0.09 ng/mL (Medizym® Tg Rem ELISA; Medipan, Blankenfelde-Mahlow, Germany) was used. As described in the current ATA guidelines ( 1 , 8 ), a suppressed Tg <0.2 ng/mL was chosen as

Article Type:
Research Article
Collections:
Thyroid
DOI:
https://doi.org/10.1530/EC-22-0312
Volume/Issue:
Volume 12: Issue 2
Open access