Department of Endocrinology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
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( Fig. 1A , B , C and D ). Insulin content in both GSIS and SU-SIS was lower than that in vehicle alone ( Fig. 1E , F, and G ). Figure 1 Effects of palmitate and oleate on insulin synthesis and secretion of MIN6 cells. Cells were exposed to
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regulated; cumulus cells only express HSD11B1, and its expression and activity increase in mature COCs, whereas oocytes only express HSD11B2, and its expression and activity remain unchanged irrespective of oocyte maturity ( 13 ). These results indicate that
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arrow indicates the position of promoter I.4. (B) RT-PCR analysis of the expression of β-adrenergic receptors in THP-1 cells. Expression of gene transcripts of the β1-adrenergic receptor (β1-AR) and the β2-AR were examined by RT-PCR using specific
Department of Breast Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China
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Department of Surgery, Second People's Hospital of Guizhou Province, Guiyang, Guizhou, China
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T cell receptor signaling pathway. Figure 2 (A) The upregulated and downregulated genes screened by DEGs. (B) The heatmap for screening the upregulated and downregulated genes. (C) Main upregulated pathways in DEG gene enrichment by KEGG
Department of Gynecology and Obstetrics, Wenzhou People’s Hospital, Wenzhou Women and Children Health, Wenzhou, Zhejiang, China
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that testosterone induced the ER stress status in β cells. Figure 2 TE-induced ER stress in INS-1 cells and primary cultured mouse islets. Levels of XBP1(S), CHOP and DR5 mRNA in INS-1 cells (A) and primary cultured mouse islets (B) were measured
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Western blot was reduced in RDM1 -siRNA infected cells, indicating effective knockdown of the target sequence ( Fig. 3B ). Compared with cells infected with control lentivirus shCtrl, the RDM1 mRNA expression in cells infected with shRDM1 was lower
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oncogene homolog B (BRAF) mutations in LCH lesions point toward a neoplastic rather than an inflammatory nature of the disease ( 1 , 10 ). A combining hypothesis considers LCH to be an inflammatory neoplasia as LCH cells harboring oncogenic mutations
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spheroids is the excellent proven suitability of this model for drug screenings ( 38 , 39 ). Figure 1 Cluster and spheroid formation. (A) Representative image of MTT cells that spontaneously form clusters when cultured in monolayer. (B) Induction of
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released glucose-stimulated c-peptide ( Fig. 1B ) and between the serum oxytocin levels and the HOMA-β index ( Fig. 1C ). Figure 1 Decreased serum oxytocin levels are associated with β-cell dysfunction in patients with gestational diabetes mellitus
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= 0.007). (B) Picture of Western blot membrane of BeWo cells where (A). BeWo cell control group. (B) BeWo cells stimulated with 10 nM T 1 AM. ODC protein expression in BeWo trophoblast model cells after stimulation with T 3 BeWo