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Sandra Pereira Department of Physiology, University of Toronto, Toronto, Ontario, Canada

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Jessy Moore Department of Health Sciences, Brock University, St. Catharines, Ontario, Canada

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Jia-Xu Li Department of Pharmaceutical Sciences, University of Toronto, Toronto, Ontario, Canada

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Wen Qin Yu Department of Physiology, University of Toronto, Toronto, Ontario, Canada

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Husam Ghanim Division of Endocrinology, Diabetes, and Metabolism, State University of New York at Buffalo, Kaleida Health, Buffalo, New York, USA

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Filip Vlavcheski Department of Health Sciences, Brock University, St. Catharines, Ontario, Canada

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Yemisi Deborah Joseph Department of Physiology, University of Toronto, Toronto, Ontario, Canada

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Paresh Dandona Division of Endocrinology, Diabetes, and Metabolism, State University of New York at Buffalo, Kaleida Health, Buffalo, New York, USA

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Allen Volchuk Department of Physiology, University of Toronto, Toronto, Ontario, Canada
Division of Cellular and Molecular Biology, Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada

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Carolyn L Cummins Department of Pharmaceutical Sciences, University of Toronto, Toronto, Ontario, Canada

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Evangelia Tsiani Department of Health Sciences, Brock University, St. Catharines, Ontario, Canada

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Adria Giacca Department of Physiology, University of Toronto, Toronto, Ontario, Canada
Department of Medicine, University of Toronto, Toronto, Ontario, Canada
Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada
Banting and Best Diabetes Centre, University of Toronto, Toronto, Ontario, Canada

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sample was run more than once for a specific parameter, quantification results were averaged for that sample. RT-PCR analysis Quantitative RT-PCR for glucose-regulated protein 78 (GRP78) mRNA in the liver and adipose tissue samples was carried out

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0248
Volume/Issue:
Volume 10: Issue 8
Open access
Nese Cinar Department of Endocrinology and Metabolism, Hacettepe University School of Medicine, 06100 Sihhiye, Ankara, Turkey

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Alper Gurlek Department of Endocrinology and Metabolism, Hacettepe University School of Medicine, 06100 Sihhiye, Ankara, Turkey

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the extent of thyroid hormone alterations in each study. The levels of ADP mRNA in the adipose tissue are decreased in hypothyroid rats compared with controls, and this decrease is in parallel with the decrease in triiodothyronine (T 3 ), T 4 , fT 3

Article Type:
Review Article
DOI:
https://doi.org/10.1530/EC-13-0061
Volume/Issue:
Volume 2: Issue 4
Open access
A Daniel Bird Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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Spencer Greatorex Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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David Reser Department of Physiology, Monash University, Melbourne, Victoria, Australia

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Gareth G Lavery Institute of Metabolism and Systems Research, 2nd Floor IBR Tower, University of Birmingham, Birmingham, UK
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK

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Timothy J Cole Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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samples using TRIzol reagent (Invitrogen) as per the manufacturer’s instructions. cDNA was then synthesised from total RNA using the QuantiTect reverse transcriptase kit (Qiagen) as per the manufacturer’s instructions. Expression levels of mRNA in adult

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0119
Volume/Issue:
Volume 6: Issue 7
Open access
Xu-Ting Song Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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Jia-Nan Zhang Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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Duo-Wei Zhao Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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Yu-Fei Zhai Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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Qi Lu Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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Mei-Yu Qi Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China

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Ming-Hai Lu Department of Animal Science, Heilongjiang State Farms Science Technology Vocational College, Harbin, China

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Shou-Long Deng CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China

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Hong-Bing Han Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, China

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Xiu-Qin Yang Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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Yu-Chang Yao Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology

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18.0 (SPSS Inc.). All data are presented as the mean ± s.e.m. Differences in mRNA and protein expression, cell proliferation, and apoptosis were analyzed using a one-way ANOVA procedure, followed by Duncan’s post hoc test. Differences in the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0181
Volume/Issue:
Volume 10: Issue 9
Open access
Lian Hollander-Cohen Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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Benjamin Böhm Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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Krist Hausken Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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Berta Levavi-Sivan Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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previously ( 15 ). To assess the relative expression of clhcgr and cfshr mRNAs, mRNA levels were normalized against the geometric mean of two endogenous reference genes, EF1α and β-actin , using the comparative threshold cycle method (−∆CT). The mRNA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0389
Volume/Issue:
Volume 8: Issue 11
Open access
Melissa Braga Department of Internal Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA

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Zena Simmons Department of Health & Life Sciences, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA

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Keith C Norris Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA

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Monica G Ferrini Department of Internal Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA
Department of Health & Life Sciences, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA
Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA

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Jorge N Artaza Department of Internal Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA
Department of Health & Life Sciences, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA
Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA

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95°C at the rate of 0.5°C per 10 s. The samples of 25°ng cDNA were analyzed in quadruplicate in parallel with GAPDH controls; standard curves (threshold cycle vs log pg cDNA) were generated by log dilutions of standard cDNA (reverse transcribed mRNA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0008
Volume/Issue:
Volume 6: Issue 3
Open access
Anna-Pauliina Iivonen Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland

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Johanna Känsäkoski Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland

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Kirsi Vaaralahti Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland

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Taneli Raivio Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland
New Children’s Hospital, Pediatric Research Center, Helsinki University Hospital, Helsinki, Finland

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-coding RNAs that suppress gene expression by binding with 3′UTRs of their target mRNAs. Binding of a miRNA with its target mRNA induces the mRNA’s translational repression or decay ( 4 ). miRNAs from individual gene families typically target hundreds of mRNAs

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0080
Volume/Issue:
Volume 8: Issue 5
Open access
Ruixin Hu School of pharmacy, Qing Dao University, Qingdao, China

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Yanting Yuan School of pharmacy, Qing Dao University, Qingdao, China

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Chaolong Liu School of pharmacy, Qing Dao University, Qingdao, China

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Ji Zhou School of pharmacy, Qing Dao University, Qingdao, China

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Lixia Ji School of pharmacy, Qing Dao University, Qingdao, China

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Guohui Jiang School of pharmacy, Qing Dao University, Qingdao, China

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the subcutaneous adipose tissue and the bacteria are encapsulated in the adipose tissue ( 7 ). In addition, the tight junctions are damaged, and the mRNA levels of occludin and zonula occludens-1 (zo-1) are significantly downregulated in diabetic mice

Article Type:
Review Article
DOI:
https://doi.org/10.1530/EC-20-0431
Volume/Issue:
Volume 10: Issue 1
Open access
Hong Tang Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Xiaomei Jiang Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Yu Hua Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Heyue Li Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Chunlan Zhu Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Xiaobai Hao Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Minhui Yi Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Linxia Li Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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these eight genes was assessed in PCOS mice using qPCR analysis. As shown in Fig. 1B , the mRNA expression of SKP2 and NEDD4L was markedly increased in PCOS mice compared with control mice. Similar to the qPCR results, the data from western blotting

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-22-0459
Volume/Issue:
Volume 12: Issue 4
Open access
Teresa Vilariño-García Department of Medical Biochemistry, Molecular Biology and Immunology. Medical School, Virgen Macarena University Hospital, University of Seville, Seville, Spain

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Antonio Pérez-Pérez Department of Medical Biochemistry, Molecular Biology and Immunology. Medical School, Virgen Macarena University Hospital, University of Seville, Seville, Spain

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Esther Santamaría-López Valencian Infertility Institute (IVI), Seville, Spain

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Nicolás Prados Valencian Infertility Institute (IVI), Seville, Spain

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Manuel Fernández-Sánchez Valencian Infertility Institute (IVI), Seville, Spain

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Víctor Sánchez-Margalet Department of Medical Biochemistry, Molecular Biology and Immunology. Medical School, Virgen Macarena University Hospital, University of Seville, Seville, Spain

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extraction and quantitative real-time-PCR (qRT-PCR) assay Relative abundance of aromatase and Sam68 mRNA was determined by qRT-PCR. After treatment with leptin, total RNA from GCs cultures was extracted using TRISURE reagent (Bioline Co) according to the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0062
Volume/Issue:
Volume 9: Issue 6
Open access