Sara Ullsten
Sara Ullsten
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
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Sara Bohman
Sara Bohman
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
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Marie E Oskarsson
Marie E Oskarsson
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
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K Peter R Nilsson
K Peter R Nilsson
Department of Chemistry, Linköping University, Linköping, Sweden
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Gunilla T Westermark
Gunilla T Westermark
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
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Per-Ola Carlsson
Per-Ola Carlsson
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
Department of Medical Sciences, Uppsala University, Uppsala, Sweden
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Department of Medical Sciences, Uppsala University, Uppsala, Sweden
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online version of the paper at http://dx.doi.org/10.1530/EC-17-0148 . Declaration of interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported
Satoshi Inoue
Satoshi Inoue
Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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Taichi Mizushima
Taichi Mizushima
Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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Hiroki Ide
Hiroki Ide
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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Guiyang Jiang
Guiyang Jiang
Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
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James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
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Takuro Goto
Takuro Goto
Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
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James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
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Yujiro Nagata
Yujiro Nagata
Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
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James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
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George J Netto
George J Netto
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA
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James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA
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Hiroshi Miyamoto
Hiroshi Miyamoto
Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Urology, University of Rochester Medical Center, Rochester, New York, USA
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James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, New York, USA
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Urology, University of Rochester Medical Center, Rochester, New York, USA
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-AR cells pretreated with SCH 772984 (2 µM for 6 h; ‘Inh’) or C6 ceramide (50 µM for 24 h; ‘Act’) with (in media containing charcoal-stripped FBS (CS-FBS)) or without (normal FBS) additional treatment of ethanol (mock) or DHT (10 nM) for 24 h. We
Tatsuya Kondo
Tatsuya Kondo
Department of Diabetes, Metabolism and Endocrinology, Kumamoto University Hospital, Chuo-Ward, Kumamoto, Japan
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Nobukazu Miyakawa
Nobukazu Miyakawa
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Sayaka Kitano
Sayaka Kitano
Department of Diabetes, Metabolism and Endocrinology, Kumamoto University Hospital, Chuo-Ward, Kumamoto, Japan
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Takuro Watanabe
Takuro Watanabe
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Rieko Goto
Rieko Goto
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Mary Ann Suico
Mary Ann Suico
Department of Molecular Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Miki Sato
Miki Sato
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Yuki Takaki
Yuki Takaki
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Masaji Sakaguchi
Masaji Sakaguchi
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Motoyuki Igata
Motoyuki Igata
Department of Diabetes, Metabolism and Endocrinology, Kumamoto University Hospital, Chuo-Ward, Kumamoto, Japan
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Junji Kawashima
Junji Kawashima
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Hiroyuki Motoshima
Hiroyuki Motoshima
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Takeshi Matsumura
Takeshi Matsumura
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Hirofumi Kai
Hirofumi Kai
Department of Molecular Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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Eiichi Araki
Eiichi Araki
Department of Metabolic Medicine, Faculty of Life Sciences, Kumamoto University, Chuo-Ward, Kumamoto, Japan
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analysis Statistical analysis was performed with SPSS 16.0 software (IBM Corp.). Values are expressed as mean ± s.d. or media with 25th percentile to 75th percentile. All the outliners have been included in the analyses. Wilcoxon signed-rank test was
Marek Niedziela
Marek Niedziela
Department of Pediatric Endocrinology and Rheumatology, Institute of Pediatrics, Karol Jonscher’s Clinical Hospital, Poznan University of Medical Sciences, Poznan, Poland
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due to high iodine intake ( 15 , 16 ). Iodine-induced hyperthyroidism (iodine-based syndrome) is a complication after iatrogenic overexposure to iodine-containing compounds, including drugs and contrast media used in imaging studies; it can
Marra Jai Aghajani
Marra Jai Aghajani
Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
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School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
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Tao Yang
Tao Yang
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
Saint Vincent’s Clinical School, UNSW Sydney, Sydney, Australia
SydPath, Saint Vincent’s Hospital, Sydney, Australia
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Saint Vincent’s Clinical School, UNSW Sydney, Sydney, Australia
SydPath, Saint Vincent’s Hospital, Sydney, Australia
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Ulf Schmitz
Ulf Schmitz
Computational BioMedicine Laboratory Centenary Institute, The University of Sydney, Camperdown, New South Wales, Australia
Gene & Stem Cell Therapy Program Centenary Institute, The University of Sydney, Camperdown, New South Wales, Australia
Faculty of Medicine & Health, The University of Sydney, Camperdown, New South Wales, Australia
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Gene & Stem Cell Therapy Program Centenary Institute, The University of Sydney, Camperdown, New South Wales, Australia
Faculty of Medicine & Health, The University of Sydney, Camperdown, New South Wales, Australia
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Alexander James
Alexander James
Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
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Charles Eugenio McCafferty
Charles Eugenio McCafferty
Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
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School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
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Paul de Souza
Paul de Souza
Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
School of Medicine, University of Wollongong, New South Wales, Australia
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School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
School of Medicine, University of Wollongong, New South Wales, Australia
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Navin Niles
Navin Niles
Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
Department of Head & Neck Surgery, Liverpool Hospital, Liverpool, New South Wales, Australia
Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia
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School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
Department of Head & Neck Surgery, Liverpool Hospital, Liverpool, New South Wales, Australia
Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia
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Tara L Roberts
Tara L Roberts
Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
South West Sydney Clinical School, UNSW Sydney, Sydney, Australia
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School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
South West Sydney Clinical School, UNSW Sydney, Sydney, Australia
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-Aldrich) and 10% FBS (Thermo Fisher). All media were supplemented with penicillin-streptomycin (Thermo Fisher) and all cells were incubated at 37°C in 5% CO 2 . EMT was induced using recombinant human IFN-γ (300-02) (10 ng/mL, Peprotech). Live cell imaging
Kate E Lines
Kate E Lines
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Mahsa Javid
Mahsa Javid
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Anita A C Reed
Anita A C Reed
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Gerard V Walls
Gerard V Walls
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Mark Stevenson
Mark Stevenson
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Michelle Simon
Michelle Simon
MRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, UK
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Kreepa G Kooblall
Kreepa G Kooblall
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Sian E Piret
Sian E Piret
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Paul T Christie
Paul T Christie
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Paul J Newey
Paul J Newey
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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Ann-Marie Mallon
Ann-Marie Mallon
MRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, UK
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Rajesh V Thakker
Rajesh V Thakker
Academic Endocrine Unit, Radcliffe Department of Medicine, University of Oxford, Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Headington, Oxford, UK
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(Modified Mayer’s Formula, Vector Laboratories, Peterborough, UK) was applied, followed by counterstaining with 1% eosin and permanent mounting (Vector Laboratories mounting media), as previously described ( 19 ). For immunohistochemical staining, antigen
Salman Razvi
Salman Razvi
Translational and Clinical Research Institute, University of Newcastle, Newcastle-upon-Tyne, UK
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Sanaa Mrabeti
Sanaa Mrabeti
Medical Affairs EMEA, Merck Serono Middle East FZ-LLC, Dubai, United Arab Emirates
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Markus Luster
Markus Luster
Department of Nuclear Medicine, University Hospital Marburg, Marburg, Germany
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research ( 3 , 42 ). Physicians should be aware that LT3–LT4 combination therapy is promoted increasingly in the media, and patients may initiate or titrate therapy without involving their healthcare team ( 43 ). Whether the overall results of these
Josi Vidart
Josi Vidart
Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
Intensive Care Unit, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil
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Intensive Care Unit, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil
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Paula Jaskulski
Paula Jaskulski
Internal Medicine Division, Hospital de Clínicas de Porto Alegre, Internal Medicine Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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Ana Laura Kunzler
Ana Laura Kunzler
Internal Medicine Division, Hospital de Clínicas de Porto Alegre, Internal Medicine Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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Rafael Aguiar Marschner
Rafael Aguiar Marschner
Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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André Ferreira de Azeredo da Silva
André Ferreira de Azeredo da Silva
Internal Medicine Division, Hospital de Clínicas de Porto Alegre, Internal Medicine Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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Simone Magagnin Wajner
Simone Magagnin Wajner
Thyroid Section, Endocrine Division, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
Internal Medicine Division, Hospital de Clínicas de Porto Alegre, Internal Medicine Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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Internal Medicine Division, Hospital de Clínicas de Porto Alegre, Internal Medicine Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
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this setting. Supplementary materials This is linked to the online version of the paper at https://doi.org/10.1530/EC-21-0504 . Declaration of interest The authors declare that there is no conflict of interest that could be perceived as
Zhandong Lei
Zhandong Lei
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
Department of Anatomy, Shanxi Medical University, Taiyuan, China
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Department of Anatomy, Shanxi Medical University, Taiyuan, China
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Yunfei Chen
Yunfei Chen
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Jin Wang
Jin Wang
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Yan Zhang
Yan Zhang
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Wenjuan Shi
Wenjuan Shi
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Xuejiao Wang
Xuejiao Wang
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Dehai Xing
Dehai Xing
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Dongxue Li
Dongxue Li
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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Xiangying Jiao
Xiangying Jiao
Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
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prepared approximately 200 μm apart. Then, the sections were immunostained for insulin (1:6400, CST, 3014s) and scanned at 40× using a Nano Zoomer-SQ Digital slide scanner (Hamamatsu, Japan). Images were analyzed using Image-Pro Plus 6.0 software (Media
Yufen Zhao
Yufen Zhao
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Erge Namei
Erge Namei
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Bingxue Yang
Bingxue Yang
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Xiangnan Bao
Xiangnan Bao
Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, PR China
National Center of Technology Innovation for Dairy Industry, Hohhot, PR China
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National Center of Technology Innovation for Dairy Industry, Hohhot, PR China
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Wei Sun
Wei Sun
Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, PR China
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Gerile Subudeng
Gerile Subudeng
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Guifang Cao
Guifang Cao
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Haijun Li
Haijun Li
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China
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Gui Wang
Gui Wang
Animal Genetic Breeding and Reproduction Research Center, Hetao College, Bayannur, PR China
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intra-COC p-CX43 and CX43 expression by cAMP To eliminate potential interference from hormones (gonadotropin and estradiol) and serum, defined pre-IVM media containing cAMP modulators (100 µM FSK and 500 µM IBMX) were used. The intra-COC cAMP level was
