Tingting Shu
Tingting Shu
Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China
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Zhigang Lv
Zhigang Lv
Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China
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Yuchun Xie
Yuchun Xie
Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China
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Junming Tang
Junming Tang
Department of Clinical Laboratory, Yixing People Hospital, Affiliated Jiangsu University, Yixing, Wuxi, Jiangsu, China
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Xuhua Mao
Xuhua Mao
Department of Clinical Laboratory, Yixing People Hospital, Affiliated Jiangsu University, Yixing, Wuxi, Jiangsu, China
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transferred into 48-well plates (10 islets/well; 10 4 cells/well) and treated with different concentrations of glucose. Insulin content was assessed using a commercial ELISA kit (ALPCO Diagnostics) ( 24 ) in accordance with the manufacturer’s instructions
Ying Xu
Ying Xu
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Lei Li
Lei Li
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Jihong Zheng
Jihong Zheng
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Meng Wang
Meng Wang
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Bopei Jiang
Bopei Jiang
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Yue Zhai
Yue Zhai
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Liumei Lu
Liumei Lu
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Cong Zhang
Cong Zhang
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Zhe Kuang
Zhe Kuang
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Xiaomei Yang
Xiaomei Yang
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Li-Na Jin
Li-Na Jin
Department of Hematology, Changzheng Hospital, Naval Medical University, Shanghai, China
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Gufa Lin
Gufa Lin
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
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Chao Zhang
Chao Zhang
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China
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, cell surface detection by ELISA assay showed that the increasing ratio of xlMRAP2.L/S lowered the surface expression of xlMC3Rs significantly. Altogether, our results demonstrated similar pharmacological profiles of xlMC3R.L and xlMC3R.S, which could
Souad Daamouch
Souad Daamouch
Department of Medicine III and Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany
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Sylvia Thiele
Sylvia Thiele
Department of Medicine III and Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany
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Lorenz Hofbauer
Lorenz Hofbauer
Department of Medicine III and Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany
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Martina Rauner
Martina Rauner
Department of Medicine III and Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany
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markers procollagen type 1 amino-terminal-propeptide (P1NP), C-terminal telopeptide of type I collagen (CTX) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) were measured using commercially available ELISA kits from IDS (Frankfurt/Main, Germany). Dkk
Ling Zhou
Ling Zhou
Cangzhou Central Hospital, Cangzhou, Hebei, China
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Ruixue Zhang
Ruixue Zhang
Cangzhou Central Hospital, Cangzhou, Hebei, China
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Shuangyan Yang
Shuangyan Yang
Cangzhou Central Hospital, Cangzhou, Hebei, China
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Yaguang Zhang
Yaguang Zhang
Cangzhou Central Hospital, Cangzhou, Hebei, China
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Dandan Shi
Dandan Shi
Cangzhou Central Hospital, Cangzhou, Hebei, China
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, China). Superoxide dismutase (SOD) activity was detected using a xanthine oxidase technique kit (ShanghaiSolarbio Bioscience and Technology Co., Shanghai, China). ELISA Interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α) and serum
Ermina Bach
Ermina Bach
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
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Niels Møller
Niels Møller
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
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Jens Otto L Jørgensen
Jens Otto L Jørgensen
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
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Mads Buhl
Mads Buhl
The Neonatal Intensive Care Unit, Aarhus University Hospital, Aarhus, Denmark
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Holger Jon Møller
Holger Jon Møller
Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
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protocol was registered at www.clinicaltrials.gov (Nbib1452958). Consent has been obtained from each patient or subject after full explanation of the purpose and nature of all procedures used. ELISA for sCD163 We determined serum concentrations of
Qing Zhou
Qing Zhou
Department of Endocrinology, Fujian Maternity and Child Health Hospital, Fujian Children’s Hospital, Fuzhou, China
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Li Yong Zhang
Li Yong Zhang
Department of Thyroid Surgery, Minimal Invasive Center, Fujian Medical University Union Hospital, Fuzhou, China
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Mei Feng Dai
Mei Feng Dai
Department of Clinical Lab, Fujian Maternity and Child Health Hospital, Fuzhou, China
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Zhen Li
Zhen Li
Department of Endocrinology, Fujian Maternity and Child Health Hospital, Fujian Children’s Hospital, Fuzhou, China
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Chao Chun Zou
Chao Chun Zou
Department of Endocrinology, The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, China
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Hui Liu
Hui Liu
Department of Endocrinology, Fujian Maternity and Child Health Hospital, Fujian Children’s Hospital, Fuzhou, China
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, and TNF-α and interleukin-6 (IL-6) concentrations were measured using an ELISA kit (R&D Systems) according to the manufacturer’s instructions. For the in vivo experiment, serum TSH, FT3, and FT4 levels of mice were determined using an ELISA kit
Eva O Melin
Eva O Melin
Department of Clinical Sciences, Endocrinology and Diabetes, Lund University, Lund, Sweden
Department of Research and Development, Växjö, Sweden
Primary Care, Växjö, Sweden
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Department of Research and Development, Växjö, Sweden
Primary Care, Växjö, Sweden
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Jonatan Dereke
Jonatan Dereke
Department of Clinical Sciences Lund, Diabetes Research Laboratory, Lund University, Lund, Sweden
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Maria Thunander
Maria Thunander
Department of Clinical Sciences, Endocrinology and Diabetes, Lund University, Lund, Sweden
Department of Research and Development, Växjö, Sweden
Department of Internal Medicine, Endocrinology and Diabetes, Central Hospital, Växjö, Sweden
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Department of Research and Development, Växjö, Sweden
Department of Internal Medicine, Endocrinology and Diabetes, Central Hospital, Växjö, Sweden
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Magnus Hillman
Magnus Hillman
Department of Clinical Sciences Lund, Diabetes Research Laboratory, Lund University, Lund, Sweden
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). Biochemical analyses Blood samples were collected into EDTA plasma tubes (BD, Franklin Lakes, NJ, USA). Plasma sCD163 was analyzed using commercially available human enzyme-linked immunosorbent assay (ELISA) DuoSet kits and supplementary ancillary kits (R
Rasha Odeh
Rasha Odeh
Section of Pediatric Endocrinology, Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan
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Abeer Alassaf
Abeer Alassaf
Section of Pediatric Endocrinology, Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan
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Lubna Gharaibeh
Lubna Gharaibeh
School of Pharmacy, University of Jordan, Amman, Jordan
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Sarah Ibrahim
Sarah Ibrahim
Section of Pediatric Endocrinology, Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan
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Fareed Khdair Ahmad
Fareed Khdair Ahmad
Section of Pediatric Gastroenterology, Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan
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Kamel Ajlouni
Kamel Ajlouni
The National Center (Institute) for Diabetes, Endocrinology and Genetics (NCDEG), University of Jordan, Amman, Jordan
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IgA (tTG IgA) using a commercially obtained ELISA (enzyme linked immunosorbent assay) kit, anti-huTransG (Generic Assays, Dahlewitz, Germany). In NCDEG, serology was determined by tTG IgA and tTG IgG using the same method. An ELISA cut-off value of
P R van Dijk
P R van Dijk
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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S J J Logtenberg
S J J Logtenberg
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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K H Groenier
K H Groenier
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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N Kleefstra
N Kleefstra
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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H J G Bilo
H J G Bilo
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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H J Arnqvist
H J Arnqvist
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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Diabetes Centre, Departments of Internal Medicine, General Practice, Langerhans Medical Research Group, Department of Internal Medicine, Division of Cell Biology, Faculty of Health Sciences, Isala Clinics, PO Box 10400, 8000 G.K. Zwolle, The Netherlands
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Medicine of the Linköping University, Linköping, Sweden. Total IGF1 concentration was measured using a one-step ELISA after acid–ethanol extraction from its binding protein using a commercial kit (Human IGFI Quantikine ELISA Kit R&D Systems) (23) . Inter
Xiaomin Li
Xiaomin Li
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, China
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Ling Fang
Ling Fang
Department of Breast Surgery, Cheng Du Shang Jin Nan Fu Hospital, West China Hospital, Sichuan University, Chengdu, China
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Hongjiang Li
Hongjiang Li
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, China
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Xiaoqin Yang
Xiaoqin Yang
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, China
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potential genotoxic damage ( 11 , 12 , 13 , 14 , 15 ). Previous epidemiological studies of estrogen metabolism have been limited and were conducted using RIA and ELISA which have poor specificity, accuracy, and/or reproducibility ( 16 , 17 , 18 , 19
