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are shown in Table 1 . Each sample was assayed in triplicate, and relative mRNA levels were normalised to the expression levels of the housekeeping gene GAPDH . Table 1 The primers of clock genes. Genes Primer sequences (5ʹ-3ʹ
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(Applied Biosystems ). Thereafter, an RT-PCR kit (Promega) was used to determine the target mRNA content using an RT-PCR system (Applied Biosystems), according to the manufacturer’s instructions. The primers used in this study were as follows: Pgc1a , 5
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modulate diverse cellular processes, such as proliferation and metastasis by reducing the abundance of target messenger RNAs (mRNAs) ( 13 , 14 , 15 , 16 ). miR-205-5p was involved in vascularization and epithelial-to-mesenchymal transition (EMT) ( 17
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imprinting effects are expressed, and now identify those CYP2C11 required transcription factors affected by GH imprinting. In addition, we have measured mRNA and/or protein levels of male-specific CYP3A2, female-predominant CYP2C6 as well as signal transducer
Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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Key Laboratory of Sports Technique, Tactics and Physical Function of General Administration of Sport of China, Scientific Research Center, Guangzhou Sport University, Guangzhou, China
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hypoxia-induced HIF-1α activation ( 5 , 6 ). In adipose tissue, the transcription factor HIF-1α promotes the mRNA expression of pro-fibrotic genes such as lysyl oxidase (LOX), collagens I (Col1), and collagens III (Col3), consequently leading to adipose
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tissue. Unpaired Student’s t -test was used to compare GAL1 protein levels between control and GH-treated Swiss-Webster mice for each sex. When GAL1 protein/mRNA expression was compared between control and GH-treated cells, unpaired Student’s t -test
Department of Anatomy, Shanxi Medical University, Taiyuan, China
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mice (KO, catalog number: ENSMUST00000074519.12) were originally generated by GemPharmatech Co., Ltd (Nanjing, China) using CRISPR/Cas9 technology. In brief, Cas9 mRNA and gRNA (sequence, S1: 5’-ACACGGTGTGCTCCTAGCG-3’; S2: 5’-CAACCAATCAGCGAGGCCGC-3’; S3
Department of Diabetes and Endocrinology, Skåne University Hospital, Malmö, Sweden
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Department of Clinical Sciences, Lund University, Malmö, Sweden
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Department of Plastic Surgery, Skåne University Hospital, Malmö, Sweden
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Department of Ophthalmology, Skåne University Hospital, Malmö, Sweden
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Department of Diabetes and Endocrinology, Skåne University Hospital, Malmö, Sweden
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Department of Diabetes and Endocrinology, Skåne University Hospital, Malmö, Sweden
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(DMEM) (Gibco, Thermo Scientific) with 10% fetal bovine serum (FBS) (Gibco, Thermo Scientific) and passaged by trypsinization less than 12 times. In order to find the concentration of simvastatin that would give an effect on the mRNA expression of genes
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/condition/disease Tissue/cells GRα mRNA expression GRα protein expression Implications for GC sensitivity References Stress Pre/post-natal stress Humans Pre-natal stressChildhood adversity/abuse leading to adult suicide PBMCs a Hippocampus
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FGF21 mRNA expression in WAT was slightly enhanced by exercise, no significant difference in serum levels and hepatic mRNA expression of FGF21 was observed between the HFD and HFD + EXE mice ( Fig. 2A , B and C ). Compared to LFD mice, HFD mice