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://doi.org/10.1007/s00125-008-1206-6 ) 15 Ng WY Thai AC Lui KF Yeo PP Cheah JS IgG-class insulin autoantibodies in autoimmune thyroid disease . International Archives of Allergy and Applied Immunology 1990 91 431 – 436 . ( https://doi.org/10
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HUH Diagnostic Center and Helsinki University Hospital, Helsinki, Finland
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HUH Diagnostic Center and Helsinki University Hospital, Helsinki, Finland
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HUH Diagnostic Center and Helsinki University Hospital, Helsinki, Finland
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REAL ® antibody diluent (S2022, Dako/Agilent) and incubated overnight in +4°C. Secondary antibody (Vectastain Elite ® ABC kit, Sec-IgG: anti-rabbit goat-Ig and anti-mouse Horse-Ig) was diluted in normal serum and PBS and incubated for 30 min in room
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Medical Microbiology Department, College of Basic Medicine, Qingdao University, Qingdao, China
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Physiology Department, College of Basic Medicine, Qingdao University, Qingdao, China
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:2000; Abcam); β-actin (#4967, 1:4000 CST, Danvers, MA, USA) at 4°C overnight and with secondary antibodies (goat anti-rabbit IgG H&L (HRP (ab6721, Abcam, 1:2000)) at room temperature for 1 h. Bands were visualized with Immobilon Western chemiluminescent
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Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
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-tag rabbit mAb (CST) at 1:2000 dilution overnight. After washing with PBS three times, cells were incubated with goat anti-rabbit IgG (Alexa Fluor 647) (1:1000) (Abcam) for 2 h. Before coverslips were mounted and sealed, Gold Antifade Reagent with DAPI (CST
Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain
Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain
University Center of Defense of Madrid (CUD-ACD), Madrid, Spain
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Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain
Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain
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Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain
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Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain
Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain
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Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain
Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain
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Immune System Diseases-Rheumatology and Oncology Service, University Hospital Príncipe de Asturias, Alcalá de Henares, Madrid, Spain
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/100; CD4, CD8: 1/50), followed by biotinylated goat anti-mouse IgG antibody and avidin–alkaline phosphatase conjugate (1/300 and 1/200 respectively; Sigma-Aldrich). Slides were developed using Fast Red (Sigma-Aldrich) chromogenic substrate and nuclei
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Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, China
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Cruz), and FITC-conjugated anti-mouse IgG (1:400, Beyotime, Shanghai, China) was used as the secondary antibody. Images were then recorded by fluorescence microscopy (Olympus). Quantitative real-time PCR (qPCR) Total RNA was extracted from the
Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, Zhenjiang, China
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antibodies and secondary antibodies were used: rabbit anti-human β-catenin, rabbit anti-human GSK-3β, rabbit anti-human p-GSK-3β, rabbit anti-β-actin (Cell Signaling Technology), and goat anti-rabbit IgG-HRP (Santa Cruz). Western blot analysis Briefly
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blocked with 3% BSA. The tissue was incubated overnight with anti-FOXP3 antibodies (rabbit C-terminal polyclonal IgG antibody clone E18492, SpringBio ® ), anti-CD25 (mouse monoclonal antibody clone 4C9, Cell Marque ® ), anti-CD4 (rabbit monoclonal antibody
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solution (BD Biosciences). To differentiate monocytes from other leukocytes, anti-human CD14+ IgG conjugated with FITC (ab28061, Abcam) was first added and incubated on ice for 30 min followed by either surface LDLR or intracellular IDOL antibodies staining
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in both groups. Furthermore, we have previously shown no difference in the prevalence of anti-SARS-CoV-2 IgM and IgG antibody positivity between IGHD subjects and matched normal stature individuals in October 2020, during the acceleration of the