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‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation
‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation
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electrocardiogram. Persons with abnormalities in the blood tests, electrocardiogram, etc. were considered not eligible for inclusion in the study. The first and second groups were performed an oral glucose tolerance test (OGTT). It should be emphasized that all the
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about the clinical details of the patients. An oral glucose tolerance test (OGTT) was performed following an 8–10 h fasting period (1.75 g/kg, maximum 75 g anhydrous glucose). The glucose measurements included fasting plasma glucose (FPG) and a 2-h value
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-oxaloacetic transaminase (GOT). On the same day, an oral glucose tolerance test (OGTT) (75 g glucose) measuring glycaemia and insulinaemia was performed at 0, 30, 60, 90, and 120 min. The Matsuda index of insulin sensitivity (ISI-Matsuda) (10000/glucose (mg/dL) × insulin
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IR = FBG (mmol/L) × fasting insulin (FIns, UI/L)/22.5) was used for assessing IR ( 21 ). The area under the curve for glucose (AUCg) and insulin (AUCi) during the oral glucose tolerance test (OGTT) was calculated geometrically following the
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with obesity underwent an oral glucose tolerance test (OGTT). Fasting blood samples were taken to measure liver function, kidney function and blood lipids. MRI was performed to assess AT in the lower extremities. Anthropometric measurements The
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using a standardized 75 g oral glucose tolerance test (OGTT) at 24–28 weeks of pregnancy. Based on the International Association of Diabetes and Pregnancy Study Group criteria ( 14 ), GDM was diagnosed if one or more of the following abnormality were met
Danish Diabetes Academy, Odense University Hospital, Odense, Denmark
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Department of Public Health, Research Unit of Epidemiology, Aarhus University, Aarhus, Denmark
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Department of Public Health, Research Unit of Epidemiology, Aarhus University, Aarhus, Denmark
Steno Diabetes Center Aarhus, Aarhus, Denmark
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Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark
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National Institute of Public Health, University of Southern Denmark, Odense, Denmark
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Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark
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drawn. Subsequently, the participants underwent a standardized oral glucose tolerance test (OGTT), ingesting 75 g glucose dissolved in 250 mL water, and blood samples were drawn 30 and 120 min after glucose intake. Biochemical measures Following
University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia
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University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia
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University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia
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University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia
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based on dairy review. According to World Health Organization (WHO) criteria (available from: http://www.who.int/diabetes/publications/diagnosis_diabetes2006/en/ ), during OGTT, normal glucose tolerance (NGT) was defined as fasting glucose levels below
Department of Anatomy, Shanxi Medical University, Taiyuan, China
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Panel 8-Plex Assay (catalog no. 171-F7001M, BIO-RAD) according to the manufacturer’s instructions. Oral glucose tolerance test and insulin tolerance test To perform the oral glucose tolerance test (OGTT), mice were fasted overnight for 16 h
Department of Clinical Research, University of Southern Denmark, Odense, Denmark
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Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
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Department of Clinical Research, University of Southern Denmark, Odense, Denmark
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insulin secretion are evaluated on the same day after the last bout of exercise has subsided ( 19 , 20 ). This is often done using oral glucose tolerance test (OGTT)-derived surrogate markers of insulin secretion and insulin sensitivity ( 5 , 12 , 13