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Lilit Egshatyan ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Daria Kashtanova ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Anna Popenko ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Olga Tkacheva ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Alexander Tyakht ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Dmitry Alexeev ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Natalia Karamnova ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Elena Kostryukova ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation
‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation
‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Vladislav Babenko ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Maria Vakhitova ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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Sergey Boytsov ‘Research of Age and Age-Associated Conditions’ Department, Laboratory of Bioinformatics, The ‘Russian Clinical Research Center for Gerontology’, ‘Chronic Noncommunicable Diseases Primary Prevention in the Healthcare System’ Department, Moscow Institute of Physics and Technology, National Research Centre for Preventive Medicine, National Research Centre for Preventive Medicine, Building 10, Petroverigskiy Lane, Moscow RF 101000, Russian Federation

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electrocardiogram. Persons with abnormalities in the blood tests, electrocardiogram, etc. were considered not eligible for inclusion in the study. The first and second groups were performed an oral glucose tolerance test (OGTT). It should be emphasized that all the

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Maria Lola Evia-Viscarra Pediatric Endocrinology Department, Research Department, Hospital Regional de Alta Especialidad del Bajío, Boulevard Milenio 130, San Carlos la Roncha, C.P. 37660 León, Guanajuato, Mexico

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Edel Rafael Rodea-Montero Pediatric Endocrinology Department, Research Department, Hospital Regional de Alta Especialidad del Bajío, Boulevard Milenio 130, San Carlos la Roncha, C.P. 37660 León, Guanajuato, Mexico

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Evelia Apolinar-Jiménez Pediatric Endocrinology Department, Research Department, Hospital Regional de Alta Especialidad del Bajío, Boulevard Milenio 130, San Carlos la Roncha, C.P. 37660 León, Guanajuato, Mexico

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Silvia Quintana-Vargas Pediatric Endocrinology Department, Research Department, Hospital Regional de Alta Especialidad del Bajío, Boulevard Milenio 130, San Carlos la Roncha, C.P. 37660 León, Guanajuato, Mexico

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about the clinical details of the patients. An oral glucose tolerance test (OGTT) was performed following an 8–10 h fasting period (1.75 g/kg, maximum 75 g anhydrous glucose). The glucose measurements included fasting plasma glucose (FPG) and a 2-h value

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Valentina Guarnotta Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Malattie Endocrine, del Ricambio e della Nutrizione, Università di Palermo, Palermo, Italy

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Silvia Lucchese Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Malattie Endocrine, del Ricambio e della Nutrizione, Università di Palermo, Palermo, Italy

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Mariagrazia Irene Mineo Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Malattie Endocrine, del Ricambio e della Nutrizione, Università di Palermo, Palermo, Italy

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Donatella Mangione Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Ostetricia e Ginecologia, Università di Palermo, Palermo, Italy

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Renato Venezia Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Ostetricia e Ginecologia, Università di Palermo, Palermo, Italy

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Piero Luigi Almasio Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Gastroenterologia ed Epatologia, Università di Palermo, Palermo, Italy

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Carla Giordano Dipartimento di Promozione della Salute, Materno-Infantile, Medicina Interna e Specialistica di Eccellenza ‘G. D’Alessandro’ (PROMISE), Sezione di Malattie Endocrine, del Ricambio e della Nutrizione, Università di Palermo, Palermo, Italy

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-oxaloacetic transaminase (GOT). On the same day, an oral glucose tolerance test (OGTT) (75 g glucose) measuring glycaemia and insulinaemia was performed at 0, 30, 60, 90, and 120 min. The Matsuda index of insulin sensitivity (ISI-Matsuda) (10000/glucose (mg/dL) × insulin

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Xingrong Tan Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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Wenjing Hu Chongqing Prevention and Treatment Hospital for Occupational Diseases, Chongqing, China

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Shan Yang Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

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Han Dai Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

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Shangcheng Xu Chongqing Prevention and Treatment Hospital for Occupational Diseases, Chongqing, China

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Gangyi Yang Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

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Ling Li Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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Shiguo Tang Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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Yi Wang Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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IR  = FBG (mmol/L) × fasting insulin (FIns, UI/L)/22.5) was used for assessing IR ( 21 ). The area under the curve for glucose (AUCg) and insulin (AUCi) during the oral glucose tolerance test (OGTT) was calculated geometrically following the

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Xiaobing Lu Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Jiang Yue Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Qianjing Liu Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Shengyun He Department of Radiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Ying Dong Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Ming Zhang Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Yicheng Qi Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Minglan Yang Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Wang Zhang Department of Radiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Hua Xu Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Qing Lu Department of Radiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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Jing Ma Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

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with obesity underwent an oral glucose tolerance test (OGTT). Fasting blood samples were taken to measure liver function, kidney function and blood lipids. MRI was performed to assess AT in the lower extremities. Anthropometric measurements The

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Yu Lin State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Yingying Zhang State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Lei Xu Maternal and Child Health Care Hospital of Dongchangfu District, Liaocheng, People’s Republic of China

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Wei Long State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Chunjian Shan State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Hongjuan Ding State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Lianghui You State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Chun Zhao State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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Zhonghua Shi State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People’s Republic of China

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using a standardized 75 g oral glucose tolerance test (OGTT) at 24–28 weeks of pregnancy. Based on the International Association of Diabetes and Pregnancy Study Group criteria ( 14 ), GDM was diagnosed if one or more of the following abnormality were met

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Charlotte Janus Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Danish Diabetes Academy, Odense University Hospital, Odense, Denmark

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Dorte Vistisen Steno Diabetes Center Copenhagen, Gentofte, Denmark

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Hanan Amadid Steno Diabetes Center Copenhagen, Gentofte, Denmark
Department of Public Health, Research Unit of Epidemiology, Aarhus University, Aarhus, Denmark

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Daniel R Witte Danish Diabetes Academy, Odense University Hospital, Odense, Denmark
Department of Public Health, Research Unit of Epidemiology, Aarhus University, Aarhus, Denmark
Steno Diabetes Center Aarhus, Aarhus, Denmark

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Torsten Lauritzen Department of Public Health, Research Unit of Epidemiology, Aarhus University, Aarhus, Denmark

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Søren Brage MRC Epidemiology Unit, University of Cambridge, Cambridge, UK

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Anne-Louise Bjerregaard Department of Public Health, Research Unit of Epidemiology, Aarhus University, Aarhus, Denmark

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Torben Hansen Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark

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Jens J Holst Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark

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Marit E Jørgensen Steno Diabetes Center Copenhagen, Gentofte, Denmark
National Institute of Public Health, University of Southern Denmark, Odense, Denmark

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Oluf Pedersen Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark

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Kristine Færch Steno Diabetes Center Copenhagen, Gentofte, Denmark

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Signe S Torekov Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark

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drawn. Subsequently, the participants underwent a standardized oral glucose tolerance test (OGTT), ingesting 75 g glucose dissolved in 250 mL water, and blood samples were drawn 30 and 120 min after glucose intake. Biochemical measures Following

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Mojca Jensterle Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre, Ljubljana, Slovenia
University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia

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Nika Aleksandra Kravos Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre, Ljubljana, Slovenia
University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia

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Simona Ferjan Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre, Ljubljana, Slovenia
University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia

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Katja Goricar University of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Pharmacogenetics Laboratory, Ljubljana, Slovenia

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Vita Dolzan University of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Pharmacogenetics Laboratory, Ljubljana, Slovenia

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Andrej Janez Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre, Ljubljana, Slovenia
University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia

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based on dairy review. According to World Health Organization (WHO) criteria (available from: http://www.who.int/diabetes/publications/diagnosis_diabetes2006/en/ ), during OGTT, normal glucose tolerance (NGT) was defined as fasting glucose levels below

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Zhandong Lei Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China
Department of Anatomy, Shanxi Medical University, Taiyuan, China

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Yunfei Chen Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Jin Wang Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Yan Zhang Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Wenjuan Shi Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Xuejiao Wang Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Dehai Xing Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Dongxue Li Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Xiangying Jiao Key Laboratory of Cellular Physiology, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China

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Panel 8-Plex Assay (catalog no. 171-F7001M, BIO-RAD) according to the manufacturer’s instructions. Oral glucose tolerance test and insulin tolerance test To perform the oral glucose tolerance test (OGTT), mice were fasted overnight for 16 h

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Maria Houborg Petersen Steno Diabetes Center Odense, Odense University Hospital, Odense, Denmark
Department of Clinical Research, University of Southern Denmark, Odense, Denmark

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Jacob Volmer Stidsen Steno Diabetes Center Odense, Odense University Hospital, Odense, Denmark

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Martin Eisemann de Almeida Steno Diabetes Center Odense, Odense University Hospital, Odense, Denmark
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark

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Emil Kleis Wentorf Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark

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Kurt Jensen Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark

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Niels Ørtenblad Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark

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Kurt Højlund Steno Diabetes Center Odense, Odense University Hospital, Odense, Denmark
Department of Clinical Research, University of Southern Denmark, Odense, Denmark

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insulin secretion are evaluated on the same day after the last bout of exercise has subsided ( 19 , 20 ). This is often done using oral glucose tolerance test (OGTT)-derived surrogate markers of insulin secretion and insulin sensitivity ( 5 , 12 , 13

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