Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
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). The prevalence of UBE2L3-Ab and eEF1A1-Ab was 35.8 and 29.5%, respectively, in Korean T1DM by solid-phase ELISA ( 6 ). Of note, 40.9% of T1DM patients who lack GADA, the most prevalent autoantibody in T1DM, had UBE2L3-Ab and/or eEF1A1-Ab. The
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NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, Department of Science, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK‐2200 Copenhagen N, Denmark
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–36NH 2 was measured with both an in-house RIA specific for the amidated C-terminal (total GLP-1 assay, codename 89390 (12) ) and with an in-house two-site sandwich ELISA for GLP-1, using a modification of the method originally described by Wilken et
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commercially available immunoassay ELISA kit (Immutopics Inc.). The analytical parameters of the cFGF23 kit were detection limit 1.5 RU/mL and working range 1.5–660 RU/mL (intraassay CV = 1.4–2.4%, interassay CV = 2.4–4.7%) and reference interval 21.6–91 RU
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Service of Endocrinology, Laboratory of Intensive Care, Department of Microbiology and Molecular Medicine, Diabetes, Hypertension and Nutrition
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Service of Endocrinology, Laboratory of Intensive Care, Department of Microbiology and Molecular Medicine, Diabetes, Hypertension and Nutrition
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Service of Endocrinology, Laboratory of Intensive Care, Department of Microbiology and Molecular Medicine, Diabetes, Hypertension and Nutrition
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arterial catheter, and by venous puncture in healthy volunteers. Plasma FGF21 concentrations were measured using a commercial ELISA Kit (BioVendor GmbH, Heidelberg, Germany). C-reactive protein (CRP), PCT, and glucose were measured using standard methods by
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Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway
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Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway
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Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway
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against 21OH (c17, goat polyclonal IgG, Santa Cruz Biotechnology) and alkaline phosphatase-conjugated donkey anti-goat IgG secondary antibody (Santa Cruz Biotechnology). Relative quantitation of 21OH from in vitro expression ELISA plates (MaxiSorp, Nunc
School of Women’s & Children’s Health, Discipline of Obstetrics and Gynaecology, University of New South Wales, Sydney, Australia
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for another ovarian factor (perhaps unknown)? Would the association between AMH and FSH weaken if follicle size and number were included as factors in the analysis? The AMH ELISA used in the 2009 study had limited sensitivity and was only able to
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gene was carried out according to the Comparative CT Method for relative quantification of gene expression ( 23 ). Concentrations of estradiol and progesterone were measured using commercially available ELISA kits (NovaTec Immundiagnostica, Germany). A
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). Determination of GH and IGF1 concentration by ELISA Trunk blood was immediately collected in tubes for serum isolation (BD Vacutainer, BD Diagnostics, NJ, USA). Blood was centrifuged (3000 g at 4°C for 15 min) to obtain serum. Aliquots were stored at −70
Department of Gynecology and Obstetrics, Wenzhou People’s Hospital, Wenzhou Women and Children Health, Wenzhou, Zhejiang, China
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:1000 dilution), GAPDH (1:2000 dilution), and cleaved caspase3 (1:1000 dilution) were ordered from CST (Danvers, U.S.); Proinsulin MAB (D3E7) and insulin MAB (ICBTACLS) were obtained from Thermo Fisher Scientific Inc. ELISA The INS-1 cells were treated by
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medium was exchanged every 3 days, and the cells were trypsinized and reseeded at a 1:3 dilution when they reached 70–80%. For quantitative reverse transcription PCR (qPCR), Western blot, ELISA, and immunofluorescence tests, STC-1 cells were plated in six