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), follicle-stimulating hormone (FSH), testosterone, oestradiol (E2) and DHEA on the third day of the menstrual cycle and during menopause excluding pregnancy, and the glucose and insulin levels during an oral glucose tolerance test; and (3) the collection of
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, DHEA sulfate (DHEA-S)) were measured in patients (on days 3–5 of the menstrual cycle or after a progestin-induced withdrawal bleeding) with the Cobas 6000 equipment (Roche Diagnostics), using kits provided by the manufacturer. Hexokinase method (Roche
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-contrast 34.4 HU (IQR 31.1–39.5 HU). Hypercortisolism ( n = 62, 47.7%), elevated serum dehydroepiandrosterone-sulfate (DHEA-S; n = 21, 38.2%), elevated 24-h urine 17-ketosteroid ( n = 16, 32.7%), and excessive aldosterone level ( n = 14, 11.5%) within
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did not use HRT. FSH and luteinizing hormone (LH), were analyzed using ELISAs (Demeditec Diagnostics, Kiel, Germany), and the steroid hormones (17β-estradiol, estrone, progesterone, testosterone and DHEA-S) were measured using liquid chromatography
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adrenals, leading thus to the production of cortisol and DHEAS ( Fig. 2 ). In humans and primates, at term, the placenta does not express cytochrome P450c17, an enzyme necessary for estrogen synthesis from progesterone. In placental syncytiotrophoblast
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and 180 min. At t = 0 min, a 3-h infusion of human insulin (Actrapid; Novo Nordisk A/S) commenced (1.0 mU/kg total body mass/min). Plasma glucose was measured every 10 min and clamped at 5 mmol/L by a variable infusion of 20% glucose. The glucose
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-stimulating hormone (FSH), luteinizing hormone (LH), DHEA sulfate (DHEAS), estradiol (E2), total testosterone (T), sex hormone-binding globulin (SHBG), anti-Müllerian hormone (AMH) and thyroid-stimulating hormone (TSH) measurements were performed using a Cobas 6000
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/25 Data are means ± s.d. *** P < 0.001 compared to PAI. BMI, body mass index; DHEA, dehydroepiandrosterone; GC, glucocorticoid; HC, hydrocortisone. Clinical follow-up During the study period, patients attended the endocrine
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and Human Reproduction 2017 46 1 – 7 . ( https://doi.org/10.1016/j.jgyn.2016.01.002 ) 49 Schwarze JE Canales J Crosby J Ortega-Hrepich C Villa S & Pommer R . DHEA use to improve likelihood of IVF/ICSI success in patients with
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test and plasma ACTH, DHEA-S, testosterone, estrogen and aldosterone levels were obtained in order to classify hormonal excess. Overt hypercortisolism was defined as an elevated 24-h urinary cortisol excretion in combination with an abnormal response to