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Malin Nylander Department of Obstetrics and Gynecology, Herlev Gentofte Hospital, Herlev, Denmark
Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Signe Frøssing Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Internal Medicine, Endocrine Unit, Herlev Gentofte Hospital, Herlev, Denmark

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Caroline Kistorp Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Internal Medicine, Endocrine Unit, Herlev Gentofte Hospital, Herlev, Denmark

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Jens Faber Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Internal Medicine, Endocrine Unit, Herlev Gentofte Hospital, Herlev, Denmark

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Sven O Skouby Department of Obstetrics and Gynecology, Herlev Gentofte Hospital, Herlev, Denmark
Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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. Participants Participants were enrolled from social media ( www.facebook.com/PCOSkliniskforsoeg ), from private practicing gynecologists and from our outpatient PCOS clinic, securing external validity. Inclusion and exclusion criteria are published elsewhere

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-16-0113
Volume/Issue:
Volume 6: Issue 2
Open access
Wioletta Pijacka
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Morag G Hunter
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Fiona Broughton Pipkin School of Biosciences, New Maternity Unit, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK

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Martin R Luck
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-targeted genes AGTR1, AGTR2 and AGT were designed using the online program Primer3 ( http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi ). Primer sequences for β-actin were obtained from (17) and those for ACE and REN were kindly made available by

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-12-0013
Volume/Issue:
Volume 1: Issue 1
Open access
Thera P Links Division of Endocrinology, Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Trynke van der Boom Division of Endocrinology, Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Wouter T Zandee Division of Endocrinology, Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Joop D Lefrandt Division of Vascular Medicine, Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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cardiovascular events. This could be the result of a hypercoagulable state, because increased factor X activity has been measured in patients with subclinical hyperthyroidism ( 16 ). Moreover, in subclinical hyperthyroidism, an increased carotid intima-media

Article Type:
Review Article
DOI:
https://doi.org/10.1530/EC-20-0539
Volume/Issue:
Volume 10: Issue 1
Open access
Jintao Hu Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Qingbo Chen Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Xiao Ding Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Xin Zheng Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Xuefeng Tang Department of Pathology, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Song Li Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Hui Yang Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing, China

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Annexin V flow cytometry. (E) GH3 cells labeled with EDU. Representative confocal microscopy images. Scale bar represents 100 μm. (F) For protein expression analysis, we collected GH3 cells grown in media containing 1 mM ASNase alone or in combination with

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0515
Volume/Issue:
Volume 9: Issue 3
Open access
B Shahida Department of Clinical Sciences, Genomics, Diabetes and Endocrinology, Lund University, Malmö, Sweden
Department of Diabetes and Endocrinology, Skåne University Hospital, Malmö, Sweden

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P Sahlstrand Johnson Department of Oto-Rhino-Laryngology, Skåne University Hospital, Malmö, Sweden
Department of Clinical Sciences, Lund University, Malmö, Sweden

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R Jain Department of Clinical Sciences, Genomics, Diabetes and Endocrinology, Lund University, Malmö, Sweden

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H Brorson Department of Clinical Sciences, Lund University, Malmö, Sweden
Department of Plastic Surgery, Skåne University Hospital, Malmö, Sweden

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P Åsman Department of Clinical Sciences Malmö, Ophthalmology, Lund University, Malmö, Sweden
Department of Ophthalmology, Skåne University Hospital, Malmö, Sweden

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M Lantz Department of Clinical Sciences, Genomics, Diabetes and Endocrinology, Lund University, Malmö, Sweden
Department of Diabetes and Endocrinology, Skåne University Hospital, Malmö, Sweden

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T Planck Department of Clinical Sciences, Genomics, Diabetes and Endocrinology, Lund University, Malmö, Sweden
Department of Diabetes and Endocrinology, Skåne University Hospital, Malmö, Sweden

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for further experiments. Confluent OFs were pretreated with 10 µM simvastatin for 24 h and in some experiments, exposed to 10% CSE for 30–120 min. Media was then changed to serum-free Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM:F-12

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0319
Volume/Issue:
Volume 8: Issue 9
Open access
Kristin Ottarsdottir Primary Health Care, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Margareta Hellgren Primary Health Care, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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David Bock Biostatistics, School of Public Health and Community Medicine, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden

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Anna G Nilsson Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Department of Endocrinology, Sahlgrenska University Hospital, Gothenburg, Sweden

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Bledar Daka Primary Health Care, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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with its receptor, may have own properties in regulating glucose metabolism. Supplementary materials This is linked to the online version of the paper at https://doi.org/10.1530/EC-20-0141 . Declaration of interest The authors declare

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0141
Volume/Issue:
Volume 9: Issue 5
Open access
Jasmin Asberger Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Thalia Erbes Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Markus Jaeger Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Gerta Rücker Institute of Medical Biometry and Statistics, Medical Center – University of Freiburg, Freiburg, Germany

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Claudia Nöthling Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Andrea Ritter Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Kai Berner Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Ingolf Juhasz-Böss Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany

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Marc Hirschfeld Department of Obstetrics and Gynecology, Medical Center – University of Freiburg, Freiburg, Germany
Faculty of Medicine, University of Freiburg, Freiburg, Germany
Institute of Veterinary Medicine, Georg-August-University Goettingen, Goettingen, Germany

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, Germany) and the cells were counterstained with hemalaun (Merck). The colour intensity was quantified and evaluated with the software Image-Pro ® 10 (MEDIA Cybernetics, Rockville, USA), according to the manufacturer’s specifications. For better comparison

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0281
Volume/Issue:
Volume 9: Issue 12
Open access
Emanuelle Nunes-Souza Pelé Pequeno Príncipe Research Institute, Água Verde, Curitiba, Parana, Brazil
Faculdades Pequeno Príncipe, Rebouças, Curitiba, Parana, Brazil
Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC) at Universidade Federal do Paraná, Agostinho Leão Jr., Glória, Curitiba, Parana, Brazil

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Mônica Evelise Silveira Laboratório Central de Análises Clínicas, Hospital de Clínicas, Universidade Federal do Paraná, Centro, Curitiba, Paraná, Brazil

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Monalisa Castilho Mendes Pelé Pequeno Príncipe Research Institute, Água Verde, Curitiba, Parana, Brazil
Faculdades Pequeno Príncipe, Rebouças, Curitiba, Parana, Brazil
Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC) at Universidade Federal do Paraná, Agostinho Leão Jr., Glória, Curitiba, Parana, Brazil

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Seigo Nagashima Serviço de Anatomia Patológica, Hospital de Clínicas, Universidade Federal do Paraná, General Carneiro, Alto da Glória, Curitiba, Parana, Brazil
Departamento de Medicina, PUC-PR, Prado Velho, Curitiba, Parana, Brazil

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Caroline Busatta Vaz de Paula Serviço de Anatomia Patológica, Hospital de Clínicas, Universidade Federal do Paraná, General Carneiro, Alto da Glória, Curitiba, Parana, Brazil
Departamento de Medicina, PUC-PR, Prado Velho, Curitiba, Parana, Brazil

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Guilherme Guilherme Vieira Cavalcante da Silva Serviço de Anatomia Patológica, Hospital de Clínicas, Universidade Federal do Paraná, General Carneiro, Alto da Glória, Curitiba, Parana, Brazil
Departamento de Medicina, PUC-PR, Prado Velho, Curitiba, Parana, Brazil

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Giovanna Silva Barbosa Serviço de Anatomia Patológica, Hospital de Clínicas, Universidade Federal do Paraná, General Carneiro, Alto da Glória, Curitiba, Parana, Brazil
Departamento de Medicina, PUC-PR, Prado Velho, Curitiba, Parana, Brazil

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Julia Belgrowicz Martins Pelé Pequeno Príncipe Research Institute, Água Verde, Curitiba, Parana, Brazil
Faculdades Pequeno Príncipe, Rebouças, Curitiba, Parana, Brazil

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Lúcia de Noronha Serviço de Anatomia Patológica, Hospital de Clínicas, Universidade Federal do Paraná, General Carneiro, Alto da Glória, Curitiba, Parana, Brazil
Departamento de Medicina, PUC-PR, Prado Velho, Curitiba, Parana, Brazil

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Luana Lenzi Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil

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José Renato Sales Barbosa Pelé Pequeno Príncipe Research Institute, Água Verde, Curitiba, Parana, Brazil
Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC) at Universidade Federal do Paraná, Agostinho Leão Jr., Glória, Curitiba, Parana, Brazil

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Rayssa Danilow Fachin Donin Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC) at Universidade Federal do Paraná, Agostinho Leão Jr., Glória, Curitiba, Parana, Brazil

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Juliana Ferreira de Moura Pós Graduação em Microbiologia, Parasitologia e Patologia, Departamento de Patologia Básica – UFPR, Curitiba, Brazil

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Gislaine Custódio Faculdades Pequeno Príncipe, Rebouças, Curitiba, Parana, Brazil
Laboratório Central de Análises Clínicas, Hospital de Clínicas, Universidade Federal do Paraná, Centro, Curitiba, Paraná, Brazil

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Cleber Machado-Souza Pelé Pequeno Príncipe Research Institute, Água Verde, Curitiba, Parana, Brazil
Faculdades Pequeno Príncipe, Rebouças, Curitiba, Parana, Brazil
Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC) at Universidade Federal do Paraná, Agostinho Leão Jr., Glória, Curitiba, Parana, Brazil

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Enzo Lalli Institut de Pharmacologie Moléculaire et Cellulaire CNRS, Sophia Antipolis, Valbonne, France

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Bonald Cavalcante de Figueiredo Pelé Pequeno Príncipe Research Institute, Água Verde, Curitiba, Parana, Brazil
Faculdades Pequeno Príncipe, Rebouças, Curitiba, Parana, Brazil
Centro de Genética Molecular e Pesquisa do Câncer em Crianças (CEGEMPAC) at Universidade Federal do Paraná, Agostinho Leão Jr., Glória, Curitiba, Parana, Brazil
Departamento de Saúde Coletiva, Universidade Federal do Paraná, Curitiba, Paraná, Brazil

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standard image of each layer containing appropriate levels of each set of immunohistochemical signals was chosen for use as a ‘mask’, determined by the Image Pro Plus ® 4.5 morphometric analysis program (Media Cybernetics ® , Rockville, MD, USA). Tissue

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0416
Volume/Issue:
Volume 9: Issue 12
Open access
Tingting Shu Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China

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Zhigang Lv Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China

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Yuchun Xie Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China

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Junming Tang Department of Clinical Laboratory, Yixing People Hospital, Affiliated Jiangsu University, Yixing, Wuxi, Jiangsu, China

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Xuhua Mao Department of Clinical Laboratory, Yixing People Hospital, Affiliated Jiangsu University, Yixing, Wuxi, Jiangsu, China

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islet β-cell function laboratory, Jiangsu Province Official Hospital), was cultured in Dulbecco’s modified Eagles medium (Invitrogen) containing 25 mM glucose and supplemented with 15% fetal bovine serum (Invitrogen). The media was supplemented with 100

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0516
Volume/Issue:
Volume 8: Issue 3
Open access
Agnieszka Kosowska Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Enrique Gallego-Colon Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Wojciech Garczorz Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Agnieszka Kłych-Ratuszny Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Mohammad Reza F Aghdam Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Michał Woz´niak Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Andrzej Witek Department of Gynaecology and Obstetrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Agnieszka Wróblewska-Czech Department of Gynaecology and Obstetrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Anna Cygal Department of Gynaecology and Obstetrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Jerzy Wojnar Department of Internal Medicine and Oncological Chemotherapy, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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Tomasz Francuz Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

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a reduction by 14% and GLP-1 by 25% in SKOV-3 cells ( Fig. 4A ) and a reduction by 22% and GLP-1 by 19% in CAOV-3 cells ( Fig. 4D ). We also assessed the TNF-α in both cancer cell media after stimulation with CPT and incretin drugs. TNF-α protein

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0294
Volume/Issue:
Volume 6: Issue 8
Open access