Wang-shu Liu
Wang-shu Liu
Department of Endocrinology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Ling-yan Hua
Ling-yan Hua
Department of Ophthalmology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Su-xiang Zhu
Su-xiang Zhu
Department of Endocrinology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Feng Xu
Feng Xu
Department of Endocrinology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Xue-qin Wang
Xue-qin Wang
Department of Endocrinology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Chun-feng Lu
Chun-feng Lu
Department of Endocrinology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Jian-bin Su
Jian-bin Su
Department of Endocrinology, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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Feng Qi
Feng Qi
Emergency Intensive Care Unit, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, China
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. After fully centrifuged, all blood samples were stored at –80°C. Sandwich ELISA kits purchased from Elabscience (Wuhan, China) were used to measure serum SDF-1 levels. EZSACAN test During the test, patients placed their hands, feet and foreheads
Emmi Naskali
Emmi Naskali
Department of Dermatology, Allergology and Venereology, University of Helsinki, and Helsinki University Central Hospital, Helsinki, Finland
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Katja Dettmer
Katja Dettmer
Institute of Functional Genomics, University of Regensburg, Regensburg, Germany
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Peter J Oefner
Peter J Oefner
Institute of Functional Genomics, University of Regensburg, Regensburg, Germany
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Pedro A B Pereira
Pedro A B Pereira
Institute of Biotechnology, DNA Sequencing and Genomics Laboratory, University of Helsinki, Helsinki, Finland
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Kai Krohn
Kai Krohn
Clinical Research Institute HUCH Ltd, Biomedicum Helsinki 1, Helsinki, Finland
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Petri Auvinen
Petri Auvinen
Institute of Biotechnology, DNA Sequencing and Genomics Laboratory, University of Helsinki, Helsinki, Finland
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Annamari Ranki
Annamari Ranki
Department of Dermatology, Allergology and Venereology, University of Helsinki, and Helsinki University Central Hospital, Helsinki, Finland
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Nicolas Kluger
Nicolas Kluger
Department of Dermatology, Allergology and Venereology, University of Helsinki, and Helsinki University Central Hospital, Helsinki, Finland
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TPH-2 (OriGene Technologies Inc. Rockville, MD, USA) were assayed with a validated in-house ELISA as previously described in detail ( 9 ). Sera from healthy blood donors obtained from the Finnish Red Cross blood service served as reference. The cut
Charlotte Höybye
Charlotte Höybye
Patient Area Endocrinology and Nephrology, Infection and Inflammation Theme, Karolinska University Hospital, Stockholm, Sweden
Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
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Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
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Laia Faseh
Laia Faseh
Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
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Christos Himonakos
Christos Himonakos
Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
Department of Medicine, Karlstad Hospital, Karlstad, Sweden
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Department of Medicine, Karlstad Hospital, Karlstad, Sweden
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Tomasz Pielak
Tomasz Pielak
NUTOPI Sp. z o. o., Poznan, Poland
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Jesper Eugen-Olsen
Jesper Eugen-Olsen
Clinical Research Centre, Copenhagen University Hospital Hvidovre, Hvidovre, Denmark
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ranges were used to determine IGF-I SDS. Serum (S) suPAR was analysed using a commercial suPARnostic ELISA assay (ViroGates, Copenhagen, Denmark). All samples were analysed for S-suPAR at the same time and in the same batch with an interassay
Min Li
Min Li
Department of Endocrinology and Metabolism, Fudan Institute of Metabolic Diseases, Zhongshan Hospital, Fudan University, Shanghai, China
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Ying Chen
Ying Chen
Department of Endocrinology and Metabolism, Fudan Institute of Metabolic Diseases, Zhongshan Hospital, Fudan University, Shanghai, China
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Jingjing Jiang
Jingjing Jiang
Department of Endocrinology and Metabolism, Fudan Institute of Metabolic Diseases, Zhongshan Hospital, Fudan University, Shanghai, China
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Yan Lu
Yan Lu
Department of Endocrinology and Metabolism, Fudan Institute of Metabolic Diseases, Zhongshan Hospital, Fudan University, Shanghai, China
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Zhiyi Song
Zhiyi Song
Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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Shengjie Zhang
Shengjie Zhang
CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China
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Chao Sun
Chao Sun
CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China
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Hao Ying
Hao Ying
CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China
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Xiaofang Fan
Xiaofang Fan
Department of Endocrinology and Metabolism, Minhang Branch, Zhongshan Hospital, Central Hospital of Minhang District, Shanghai Minhang Hospital, Fudan University, Shanghai, China
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Yuping Song
Yuping Song
Department of Endocrinology and Metabolism, Minhang Branch, Zhongshan Hospital, Central Hospital of Minhang District, Shanghai Minhang Hospital, Fudan University, Shanghai, China
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Jialin Yang
Jialin Yang
Department of Endocrinology and Metabolism, Minhang Branch, Zhongshan Hospital, Central Hospital of Minhang District, Shanghai Minhang Hospital, Fudan University, Shanghai, China
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Lin Zhao
Lin Zhao
Department of Endocrinology and Metabolism, Fudan Institute of Metabolic Diseases, Zhongshan Hospital, Fudan University, Shanghai, China
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glucose (mmol/L)/22.5. Serum Nrg4 concentrations were measured using ELISA kits from Phoenix Pharmaceuticals Inc. (EK-056-24). Animal experiments Male C57BL/6 mice aged 8–10 weeks were housed at 22 ± 1°C with a humidity of 35 ± 5% under a 12-h
Yongping Liu
Yongping Liu
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Shuo Wang
Shuo Wang
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Qingling Guo
Qingling Guo
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Yongze Li
Yongze Li
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Jing Qin
Jing Qin
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Na Zhao
Na Zhao
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Yushu Li
Yushu Li
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Zhongyan Shan
Zhongyan Shan
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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Weiping Teng
Weiping Teng
Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, People’s Republic of China
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A level Serum Sema 5A levels were measured with ELISA kits (SEL924Hu) according to the manufacturer’s instructions (CLOUD-CLONE CORP, Wuhan, China). The intra-assay CV value is less than 10% and the inter-assay CV value is less than 12
Xiaoli Liu
Xiaoli Liu
Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, China
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Lanxiang Liu
Lanxiang Liu
Department of Medical Imaging, The First Hospital of Qinhuangdao, Qinhuangdao, China
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Rui Wang
Rui Wang
Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, China
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Xiaojiao Jia
Xiaojiao Jia
Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, China
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Binbin Liu
Binbin Liu
Department of Functional Examination, The First Hospital of Qinhuangdao, Qinhuangdao, China
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Ning Ma
Ning Ma
Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, China
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Qiang Lu
Qiang Lu
Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, China
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at −80°C for testing. An ELISA was used for detecting the FGF21. The kit was provided by BIM Company (USA), and the detection was performed according to the manufacturer’s instructions. The serum insulin level was detected using a chemiluminescence
Tao Gao
Tao Gao
Department of General Practice, The Second Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China
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Rui Liu
Rui Liu
Department of Oncology. The Second Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China
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Chunli Li
Chunli Li
Institute of Life Sciences, Chongqing Medical University, Chongqing, China
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Xinglin Chu
Xinglin Chu
Department of General Practice, The Second Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China
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Qiao Guo
Qiao Guo
Department of General Practice, The Second Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China
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Dazhi Ke
Dazhi Ke
Department of General Practice, The Second Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, China
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determined by commercial ELISA kits following the manufacturer’s protocol (Jingmei Engineering, Jiangsu Province, China). For all kits, the interassay and intra-assay coefficients of variation were <12 and < 8%, respectively. The minimum detectable
Shenghe Luo
Shenghe Luo
College of Pharmacy, Yanbian University, Yanji, China
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Yunhui Zuo
Yunhui Zuo
Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, China
Department of Cardiology, Yanbian University Hospital, Yanji, China
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Department of Cardiology, Yanbian University Hospital, Yanji, China
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Xiaotian Cui
Xiaotian Cui
Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, China
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Meiping Zhang
Meiping Zhang
Department of Cardiology, Yanbian University Hospital, Yanji, China
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Honghua Jin
Honghua Jin
Department of Pharmacy, Yanbian University Hospital, Yanji, China
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Lan Hong
Lan Hong
Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, China
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tissue wet weight (−80℃ was placed after anticoagulant treatment with aprotinin and EDTA in an ANP radioimmunoassay kit before collection of human, rat perfusate, and mouse plasma). ELISA Plasma from 163 patients was subjected to ELISA experiments
Xiaonan Guo
Xiaonan Guo
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Wenjing Hu
Wenjing Hu
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Xiaorui Lyu
Xiaorui Lyu
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Hanyuan Xu
Hanyuan Xu
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Huijuan Zhu
Huijuan Zhu
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Hui Pan
Hui Pan
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Linjie Wang
Linjie Wang
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Hongbo Yang
Hongbo Yang
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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Fengying Gong
Fengying Gong
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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collection All blood samples were collected from the abdominal aorta and immediately centrifuged at 3000 g for 10 min at 4°C under anesthesia before sacrifice. Serum IGF-1 levels were measured with ELISA kits (Cloud-Clone Corp., Wuhan, China) according
Beril Erdem
Beril Erdem
Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey
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Angela Schulz
Angela Schulz
Rudolf Schönheimer Institute of Biochemistry, Faculty of Medicine, Leipzig University, Leipzig, Germany
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Emel Saglar
Emel Saglar
Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey
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Ferhat Deniz
Ferhat Deniz
Department of Endocrinology, SBÜ Sultan Abdülhamid Han Teaching Hospital, Istanbul, Turkey
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Torsten Schöneberg
Torsten Schöneberg
Rudolf Schönheimer Institute of Biochemistry, Faculty of Medicine, Leipzig University, Leipzig, Germany
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Hatice Mergen
Hatice Mergen
Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey
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former described pcD-ps vector with a removed poly-A fragment 3′ of the MSC-cloning region ( 9 ). The correctness of the mutant V2 receptor plasmids was verified by sequencing. For receptor detection by ELISA, wild-type (WT) and all mutant AVPR2-coding
