Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
Unit of Cardiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
Unit of Cardiology, Karolinska University Hospital, Stockholm, Sweden
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Unit of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden
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susceptibility to respiratory tract infections. The diagnostic entities included IgA- and IgG-subclass deficiency. No statin treatment was allowed but other medications such as anti-hypertensive and anti-depressant drugs were permitted. The genotyping had been
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-rabbit IgG antibody (Sigma-Aldrich) and ECL reagent using an ImageQuant LAS4000 (GE Health Care). Enzymatic removal and modification of N -glycans Purified CBG (>90% purity, Affiland, Liége, Belgium) was reconstituted and 10 µg in 20 µL Tris–HCL, pH
Endocrine Unit, Royal Victoria Infirmary, Newcastle upon Tyne, UK
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Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
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Endocrine Unit, Royal Victoria Infirmary, Newcastle upon Tyne, UK
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ApoTome attachment). A negative control, without primary antibodies, was used to identify background autofluorescence and also to check for nonspecific binding. The isotype-matched species antibodies (Santa Cruz Biotechnology) were also used as IgG isotype
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sporadic patients with PC ( 20 , 21 ), which may assist in diagnosing PC. However, the absence of CDC73 mutations or loss of parafibromin staining cannot exclude PC ( 22 ). A recent study revealed that cancer-derived immunoglobulin G (cancer- IgG
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Royal Marsden Hospital, London, UK
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New onset hypocalcemia Activating antibodies to the calcium-sensing receptor (CaSR) , of IgG1 and IgG3 subclasses with affinity to functional epitopes on the receptor, thus causing hypocalcaemia Albumin corrected calcium below 2.1 mmol/L with low
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., Ltd., Item No. JL27073, JL12201, JL13251, JL12462). β-actin protein antibody, horseradish peroxidase (HRP) enzyme-labeled goat anti-rabbit immunoglobulin G (IgG), ultrasensitive electrogenerated chemiluminescence (ECL) chemiluminescence kit, animal
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for 130 out of 149 cases. Ki67 immunostaining was performed automatically by the Ventana Benchmark immunostaining system (Ventana Medical Systems, Inc., Tucson, AZ) using a rabbit monoclonal primary antibody (immunoglobulin (Ig)G) directed against the
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20 Autoimmune adrenalitis Vitiligo Hydrocortisone 32.5 mg/day 31 8 F 69 Autoimmune adrenalitis Hypertensive cardiopathy, gastroesophageal reflux, chronic atrophic gastritis, IgG-lambda paraproteinemia Cortisone acetate 25 mg
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IgA (tTG IgA) using a commercially obtained ELISA (enzyme linked immunosorbent assay) kit, anti-huTransG (Generic Assays, Dahlewitz, Germany). In NCDEG, serology was determined by tTG IgA and tTG IgG using the same method. An ELISA cut-off value of
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-rabbit IgG, cat. no. 7074, Cell Signalling) at RT for 1 h. All antibodies were diluted in a 1% fat-free skimmed milk and 0.05% TBS-Tween solution. Between stages, the membranes were washed with 0.05% TBS-Tween. Protein bands were visualised, with horseradish