Riccardo Pofi
Riccardo Pofi
Department of Endocrinology, Oxford Centre for Diabetes, Endocrinology and Metabolism and NIHR Oxford Biomedical Research Centre, Churchill Hospital, University of Oxford, Oxford, UK
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Ilaria Bonaventura
Ilaria Bonaventura
Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena, Rome, Italy
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Joanne Duffy
Joanne Duffy
Department of Clinical Chemistry and Immunology, Heartlands Hospital, Birmingham, UK
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Zoe Maunsell
Zoe Maunsell
Department of Clinical Biochemistry, Oxford University Hospitals NHS Foundation Trust, Oxford, UK
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Brian Shine
Brian Shine
Department of Clinical Biochemistry, Oxford University Hospitals NHS Foundation Trust, Oxford, UK
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Andrea M Isidori
Andrea M Isidori
Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena, Rome, Italy
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Jeremy W Tomlinson
Jeremy W Tomlinson
Department of Endocrinology, Oxford Centre for Diabetes, Endocrinology and Metabolism and NIHR Oxford Biomedical Research Centre, Churchill Hospital, University of Oxford, Oxford, UK
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–liquid extraction by isotope dilution liquid chromatography-tandem mass spectrometry. Deuterated internal standard (Fludrocortisone D5, Sigma) was added to each sample, QC and calibrator followed by diethylether. Samples were mixed and then placed into an ethanol
Simon Chang
Simon Chang
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Odense, Denmark
Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Internal Medicine, Lillebaelt Hospital, Kolding, Denmark
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Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Internal Medicine, Lillebaelt Hospital, Kolding, Denmark
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Arkadiusz J Goszczak
Arkadiusz J Goszczak
NanoSYD, The Mads Clausen Institute, University of Southern Denmark, Sønderborg, Denmark
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Anne Skakkebæk
Anne Skakkebæk
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark
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Department of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark
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Jens Fedder
Jens Fedder
Centre of Andrology and Fertility Clinic, Odense University Hospital, Odense, Denmark
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Anders Bojesen
Anders Bojesen
Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark
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M Vakur Bor
M Vakur Bor
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Odense, Denmark
Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
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Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
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Moniek P M de Maat
Moniek P M de Maat
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Odense, Denmark
Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
Department of Haematology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands
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Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
Department of Haematology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands
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Claus H Gravholt
Claus H Gravholt
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark
Department of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark
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Department of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark
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Anna-Marie B Münster
Anna-Marie B Münster
Unit for Thrombosis Research, Department of Regional Health Research, University of Southern Denmark, Odense, Denmark
Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
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Department of Clinical Biochemistry, Hospital of South West Jutland, Esbjerg, Denmark
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using DiameterJ 1.018, a validated ( 20 ) open-source software package ( https://imagej.net/DiameterJ ). Other quantities Sex hormones were assessed by liquid chromatography-tandem mass spectrometry. For testosterone, the limit of detection was 0
I Savchuk
I Savchuk
Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden
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M L Morvan
M L Morvan
LUNAM Université, École Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Nantes-Atlantique (Oniris), Laboratoire d’Étude des Résidus et Contaminants dans les Aliments (LABERCA), USC INRA 1329, Nantes, France
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J P Antignac
J P Antignac
LUNAM Université, École Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Nantes-Atlantique (Oniris), Laboratoire d’Étude des Résidus et Contaminants dans les Aliments (LABERCA), USC INRA 1329, Nantes, France
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K Gemzell-Danielsson
K Gemzell-Danielsson
Department of Obstetrics and Gynecology, Karolinska Institute & University Hospital, Stockholm, Sweden
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B Le Bizec
B Le Bizec
LUNAM Université, École Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Nantes-Atlantique (Oniris), Laboratoire d’Étude des Résidus et Contaminants dans les Aliments (LABERCA), USC INRA 1329, Nantes, France
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O Söder
O Söder
Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden
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K Svechnikov
K Svechnikov
Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden
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Skakkebaek NE Juul A Le Bizec B. Assessment of circulating sex steroid levels in prepubertal and pubertal boys and girls by a novel ultrasensitive gas chromatography-tandem mass spectrometry method . Journal of Clinical Endocrinology and
Angelica Sharma
Angelica Sharma
Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Katharine Lazarus
Katharine Lazarus
Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Deborah Papadopoulou
Deborah Papadopoulou
Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Hemanth Prabhudev
Hemanth Prabhudev
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Tricia Tan
Tricia Tan
Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
Department of Clinical Biochemistry, North West London Pathology, London, UK
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Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
Department of Clinical Biochemistry, North West London Pathology, London, UK
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Karim Meeran
Karim Meeran
Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
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Sirazum Choudhury
Sirazum Choudhury
Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
Department of Clinical Biochemistry, North West London Pathology, London, UK
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Department of Endocrinology, Imperial College Healthcare NHS Trust, London, UK
Department of Clinical Biochemistry, North West London Pathology, London, UK
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mass spectrometry (Waters Quattro Premier Mass Spectrometer). The assay is controlled using custom internal quality controls and is a UKAS-accredited assay. This method has demonstrated intra-assay and inter-assay coefficient variations of 2.7 and 4
Emmi Naskali
Emmi Naskali
Department of Dermatology, Allergology and Venereology, University of Helsinki, and Helsinki University Central Hospital, Helsinki, Finland
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Katja Dettmer
Katja Dettmer
Institute of Functional Genomics, University of Regensburg, Regensburg, Germany
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Peter J Oefner
Peter J Oefner
Institute of Functional Genomics, University of Regensburg, Regensburg, Germany
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Pedro A B Pereira
Pedro A B Pereira
Institute of Biotechnology, DNA Sequencing and Genomics Laboratory, University of Helsinki, Helsinki, Finland
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Kai Krohn
Kai Krohn
Clinical Research Institute HUCH Ltd, Biomedicum Helsinki 1, Helsinki, Finland
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Petri Auvinen
Petri Auvinen
Institute of Biotechnology, DNA Sequencing and Genomics Laboratory, University of Helsinki, Helsinki, Finland
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Annamari Ranki
Annamari Ranki
Department of Dermatology, Allergology and Venereology, University of Helsinki, and Helsinki University Central Hospital, Helsinki, Finland
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Nicolas Kluger
Nicolas Kluger
Department of Dermatology, Allergology and Venereology, University of Helsinki, and Helsinki University Central Hospital, Helsinki, Finland
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separated by centrifugation and stored at −80°C. For the determination of 5-HT, L-DOPA and various Trp metabolites by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), the frozen serum samples were sent on dry ice to the
L Ghataore
L Ghataore
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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I Chakraborti
I Chakraborti
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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S J Aylwin
S J Aylwin
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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K-M Schulte
K-M Schulte
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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D Dworakowska
D Dworakowska
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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P Coskeran
P Coskeran
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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N F Taylor
N F Taylor
Departments of, Clinical Biochemistry, Medicine, Department of Endocrinology and Internal Medicine, King's College Hospital, London SE5 9RS, UK
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conjugates were hydrolysed enzymatically using Helix pomatia digestive juice. The free steroid products were then re-extracted and methyl oxime–trimethylsilyl ether (MO–TMS) derivatives were prepared before analysis by gas chromatography–mass spectrometry
Filippo Ceccato
Filippo Ceccato
Department of Medicine DIMED, University of Padova, Padova, Italy
Endocrine Disease Unit, University-Hospital of Padova, Padova, Italy
Department of Neuroscience DNS, University of Padova, Padova, Italy
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Endocrine Disease Unit, University-Hospital of Padova, Padova, Italy
Department of Neuroscience DNS, University of Padova, Padova, Italy
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Elisa Selmin
Elisa Selmin
Department of Medicine DIMED, University of Padova, Padova, Italy
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Giorgia Antonelli
Giorgia Antonelli
Department of Medicine DIMED, University of Padova, Padova, Italy
Laboratory Medicine, University-Hospital of Padova, Padova, Italy
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Laboratory Medicine, University-Hospital of Padova, Padova, Italy
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Mattia Barbot
Mattia Barbot
Department of Medicine DIMED, University of Padova, Padova, Italy
Endocrine Disease Unit, University-Hospital of Padova, Padova, Italy
Department of Neuroscience DNS, University of Padova, Padova, Italy
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Endocrine Disease Unit, University-Hospital of Padova, Padova, Italy
Department of Neuroscience DNS, University of Padova, Padova, Italy
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Andrea Daniele
Andrea Daniele
Department of Medicine DIMED, University of Padova, Padova, Italy
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Marco Boscaro
Marco Boscaro
Department of Medicine DIMED, University of Padova, Padova, Italy
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Mario Plebani
Mario Plebani
Department of Medicine DIMED, University of Padova, Padova, Italy
Laboratory Medicine, University-Hospital of Padova, Padova, Italy
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Laboratory Medicine, University-Hospital of Padova, Padova, Italy
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Carla Scaroni
Carla Scaroni
Department of Medicine DIMED, University of Padova, Padova, Italy
Endocrine Disease Unit, University-Hospital of Padova, Padova, Italy
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Endocrine Disease Unit, University-Hospital of Padova, Padova, Italy
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), because outdated radioimmunoassays were not able to differentiate compounds with structural similarity to the target molecule as cortisone (E) and F ( 9 , 10 ). The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasing, and
Kristian Almstrup
Kristian Almstrup
Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Hanne Frederiksen
Hanne Frederiksen
Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Anna-Maria Andersson
Anna-Maria Andersson
Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Anders Juul
Anders Juul
Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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ArrayExpress repository ( www.ebi.ac.uk/arrayexpress ), under accession number E-MTAB-4187. Measurements of EDCs in urine In this study, we included data from liquid-chromatography tandem-mass-spectrometry (LC-MS/MS) measurements of triclosan (TCS
Anela Blažević
Anela Blažević
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Anand M Iyer
Anand M Iyer
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Marie-Louise F van Velthuysen
Marie-Louise F van Velthuysen
Department of Pathology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Johannes Hofland
Johannes Hofland
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Peter M van Koestveld
Peter M van Koestveld
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Gaston J H Franssen
Gaston J H Franssen
Department of Surgery, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Richard A Feelders
Richard A Feelders
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Marina Zajec
Marina Zajec
Laboratory of Neuro-Oncology/Clinical & Cancer Proteomics, Department of Neurology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Theo M Luider
Theo M Luider
Laboratory of Neuro-Oncology/Clinical & Cancer Proteomics, Department of Neurology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Wouter W de Herder
Wouter W de Herder
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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Leo J Hofland
Leo J Hofland
Department of Internal Medicine, Section Endocrinology, Erasmus University Medical Center and Erasmus MC Cancer Institute, Rotterdam, Netherlands
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involved in the tryptophan metabolism by liquid chromatography−mass spectrometry (LC-MS). Methods Sample selection Patients were included from the Erasmus Medical Center NET database. The study was performed retrospectively, and, according to
Florian Schederecker
Florian Schederecker
Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
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Alexander Cecil
Alexander Cecil
Molecular Endocrinology and Metabolism, Genome Analysis Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
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Cornelia Prehn
Cornelia Prehn
Molecular Endocrinology and Metabolism, Genome Analysis Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
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Jana Nano
Jana Nano
Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
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Wolfgang Koenig
Wolfgang Koenig
Institute of Epidemiology and Medical Biometry, University of Ulm, Ulm, Germany
Deutsches Herzzentrum München, Technische Universität München, DZHK (German Centre for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany
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Deutsches Herzzentrum München, Technische Universität München, DZHK (German Centre for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany
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Jerzy Adamski
Jerzy Adamski
Molecular Endocrinology and Metabolism, Genome Analysis Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, Germany
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
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Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, Germany
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
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Tanja Zeller
Tanja Zeller
Department of General and Interventional Cardiology, University Heart Center Hamburg, Hamburg, Germany
German Center for Cardiovascular Research (DZHK), Partner Site Hamburg/Kiel/Lübeck, Germany
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German Center for Cardiovascular Research (DZHK), Partner Site Hamburg/Kiel/Lübeck, Germany
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Annette Peters
Annette Peters
Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
German Centre for Cardiovascular Research (DZHK), Partner Site Munich, Munich, Germany
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German Centre for Cardiovascular Research (DZHK), Partner Site Munich, Munich, Germany
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Barbara Thorand
Barbara Thorand
Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
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/active) and education in years ( 36 ). Further, we used estimated glomerular filtration rate (eGFR) which was calculated from serum creatinine (isotope dilution mass spectrometry-calibrated) with the ‘Chronic Kidney Disease Epidemiology Collaboration’ (CKD
