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, betatrophin and adiponectin levels were determined by using ELISA kits from the CUSABIO Life Science, Inc. (Cat#: CSB-EQ027943HU, China) and Phoenix Pharmaceuticals, Inc. (Cat#: EK-067-29,USA) for irisin, Phoenix Pharmaceuticals Inc. (Cat#: EK-051-60,USA) for
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Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
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Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
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Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
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Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
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State Key Laboratory of Pharmaceutical Biotechnology, University of Hong Kong, Hong Kong, Hong Kong
Department of Pharmacy and Pharmacology, LKS Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong
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Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Chinese University of Hong Kong-Shanghai Jiao Tong University Joint Research Centre in Diabetes Genomics and Precision Medicine, Hong Kong, Hong Kong
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Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Chinese University of Hong Kong-Shanghai Jiao Tong University Joint Research Centre in Diabetes Genomics and Precision Medicine, Hong Kong, Hong Kong
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Diagnostics Corp.). Insulin levels in the PCOS group were tested using an automated chemiluminescent immunoassay analyser (Siemens Healthcare Diagnostics Inc.), while ELISA kits (Dako Denmark A/S) were used to measure the insulin levels of the control group
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centrifuged at 1000 g for 15 min, and serum was removed from SSTs and then stored at −80°C in preparation for the assays. Serum TNF-α and its soluble receptors were measured by the quantitative technique of a ‘sandwich’ ELISA. Circulating TNF-α levels were
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-matched Caucasian, nonobese, nondiabetic controls, using a sensitive assay. Recently, we published the results of a noninterventional study in which we compared two different, commercially available, ELISA formats of AG and UAG in venous plasma stabilized or not
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-house sandwich ELISA on a BEP-2000 ELISA analyser (Dade Behring, Inc., Marburg, Germany) (32) . In each run of 36 samples, we co-analyzed control samples and serum calibrator with concentrations traceable to purified CD163. The interassay imprecision in the
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Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland
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Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland
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Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland
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Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland
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Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland
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sandwich ELISA kit (R&D Systems, DGD150). The ELISA was performed according to the manufacturer’s instructions. The assay sensitivity was 4.39 pg/mL, and the intra- and inter-assay coefficient variations (CV) were <3% and <6%, respectively
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-associated protein proliferating cell nuclear antigen (PCNA) and the apoptosis-associated proteins BCL-XL and BAX were measured using respective ELISA kits (Meimian, Jianshu, China) according to the manufacturer instructions. Key ceRNA network construction and
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of diagnosis of the GEP NET (baseline). The sCgA levels were measured using a commercially available ELISA method (CIS Bio International, Gif-sur-Yvette cedex, France; upper limit of normal (ULN) 94 μg/l). ‘Nonelevated’ sCgA was defined as ≤2× the ULN
Division of Endocrinology and Metabolism, Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
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NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK
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NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK
National Institute for Health Research (NIHR) Health Protection Research Unit in Environmental Exposures and Health, Imperial College London, London, UK
Mohn Centre for Children’s Health and Wellbeing, Imperial College London, London, UK
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NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK
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NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK
National Institute for Health Research (NIHR) Health Protection Research Unit in Environmental Exposures and Health, Imperial College London, London, UK
Mohn Centre for Children’s Health and Wellbeing, Imperial College London, London, UK
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sample was analysed at 4 further time points up to a maximum of 608 days for steroids assayed by LC-MS/MS and 664 days for steroids assayed by ELISA immunoassay (Salimetrics Assay, State College, PA, USA). Saliva samples for males were analysed for
Graduate School, Wannan Medical College, Wuhu, Anhui, People’s Republic of China
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Graduate School, Wannan Medical College, Wuhu, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
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equation. Serum LCN2 levels were assayed using an ELISA kit (Shanghai Xitang Biotechnology Co. Ltd, Shanghai, China). Procedures were according to the manual instructions by kit provider. Assessment of DPN DPN was diagnosed using TCNS based on