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Xingrong Tan Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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Wenjing Hu Chongqing Prevention and Treatment Hospital for Occupational Diseases, Chongqing, China

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Shan Yang Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

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Han Dai Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

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Shangcheng Xu Chongqing Prevention and Treatment Hospital for Occupational Diseases, Chongqing, China

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Gangyi Yang Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

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Ling Li Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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Shiguo Tang Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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Yi Wang Department of Endocrinology, 9th People’s Hospital of Chongqing, Beibei City, Chongqong, China

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, betatrophin and adiponectin levels were determined by using ELISA kits from the CUSABIO Life Science, Inc. (Cat#: CSB-EQ027943HU, China) and Phoenix Pharmaceuticals, Inc. (Cat#: EK-067-29,USA) for irisin, Phoenix Pharmaceuticals Inc. (Cat#: EK-051-60,USA) for

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0569
Volume/Issue:
Volume 10: Issue 1
Open access
Feifei Cheng Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Noel Yat Hey Ng Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Claudia Ha Ting Tam Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong
Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Yuying Zhang Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong
Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Cadmon King Poo Lim Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Guozhi Jiang Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong
Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Alex Chi Wai Ng Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Tiffany Tse Ling Yau Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Lai Ping Cheung Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong, Hong Kong

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Aimin Xu Department of Medicine, Li Ka Shing (LKS) Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong
State Key Laboratory of Pharmaceutical Biotechnology, University of Hong Kong, Hong Kong, Hong Kong
Department of Pharmacy and Pharmacology, LKS Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong

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Juliana C N Chan Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong
Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Chinese University of Hong Kong-Shanghai Jiao Tong University Joint Research Centre in Diabetes Genomics and Precision Medicine, Hong Kong, Hong Kong

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Ronald C W Ma Department of Medicine and Therapeutics The Chinese University of Hong Kong, Hong Kong, Hong Kong
Hong Kong Institute of Diabetes and Obesity, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
Chinese University of Hong Kong-Shanghai Jiao Tong University Joint Research Centre in Diabetes Genomics and Precision Medicine, Hong Kong, Hong Kong

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Diagnostics Corp.). Insulin levels in the PCOS group were tested using an automated chemiluminescent immunoassay analyser (Siemens Healthcare Diagnostics Inc.), while ELISA kits (Dako Denmark A/S) were used to measure the insulin levels of the control group

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0082
Volume/Issue:
Volume 10: Issue 10
Open access
Qing Zhu Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Jianbin Su Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Xueqin Wang Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Mengjie Tang Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Yingying Gao Department of Rheumatology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Dongmei Zhang Clinical Medicine Research Center, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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centrifuged at 1000 g for 15 min, and serum was removed from SSTs and then stored at −80°C in preparation for the assays. Serum TNF-α and its soluble receptors were measured by the quantitative technique of a ‘sandwich’ ELISA. Circulating TNF-α levels were

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0162
Volume/Issue:
Volume 9: Issue 7
Open access
Roxanne C S van Adrichem Department of Internal Medicine, Sector of Endocrinology, ENETS Centre of Excellence for Neuroendocrine Tumors, Erasmus MC, Rotterdam, The Netherlands

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Aart Jan van der Lely Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands

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Martin Huisman Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands

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Piet Kramer Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands

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Richard A Feelders Department of Internal Medicine, Sector of Endocrinology, ENETS Centre of Excellence for Neuroendocrine Tumors, Erasmus MC, Rotterdam, The Netherlands

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Patric J D Delhanty Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands

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Wouter W de Herder Department of Internal Medicine, Sector of Endocrinology, ENETS Centre of Excellence for Neuroendocrine Tumors, Erasmus MC, Rotterdam, The Netherlands

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-matched Caucasian, nonobese, nondiabetic controls, using a sensitive assay. Recently, we published the results of a noninterventional study in which we compared two different, commercially available, ELISA formats of AG and UAG in venous plasma stabilized or not

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-16-0021
Volume/Issue:
Volume 5: Issue 4
Open access
Lars Peter Sørensen
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Tina Parkner Department of Endocrinology and Internal Medicine, Department of Clinical Biochemistry, Department of Biostatistics, Department of Clinical Biochemistry, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark

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Esben Søndergaard
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Bo Martin Bibby Department of Endocrinology and Internal Medicine, Department of Clinical Biochemistry, Department of Biostatistics, Department of Clinical Biochemistry, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark

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Holger Jon Møller Department of Endocrinology and Internal Medicine, Department of Clinical Biochemistry, Department of Biostatistics, Department of Clinical Biochemistry, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark

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Søren Nielsen
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-house sandwich ELISA on a BEP-2000 ELISA analyser (Dade Behring, Inc., Marburg, Germany) (32) . In each run of 36 samples, we co-analyzed control samples and serum calibrator with concentrations traceable to purified CD163. The interassay imprecision in the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-14-0107
Volume/Issue:
Volume 4: Issue 1
Open access
Mohamed Asrih Service of Endocrinology, Diabetes, Nutrition and Patient Therapeutic Education, Geneva University Hospitals, Geneva, Switzerland

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Flore Sinturel Thoracic and Endocrine Surgery Division, Department of Surgery, Geneva University Hospitals (HUG), Geneva, Switzerland
Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland

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Richard Dubos Department and Division of Primary Care Medicine, Geneva University Hospitals (HUG), Geneva, Switzerland

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Idris Guessous Department and Division of Primary Care Medicine, Geneva University Hospitals (HUG), Geneva, Switzerland

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Zoltan Pataky Service of Endocrinology, Diabetes, Nutrition and Patient Therapeutic Education, Geneva University Hospitals, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland

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Charna Dibner Thoracic and Endocrine Surgery Division, Department of Surgery, Geneva University Hospitals (HUG), Geneva, Switzerland
Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland

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François R Jornayvaz Service of Endocrinology, Diabetes, Nutrition and Patient Therapeutic Education, Geneva University Hospitals, Geneva, Switzerland
Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland

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Karim Gariani Service of Endocrinology, Diabetes, Nutrition and Patient Therapeutic Education, Geneva University Hospitals, Geneva, Switzerland
Diabetes Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland

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sandwich ELISA kit (R&D Systems, DGD150). The ELISA was performed according to the manufacturer’s instructions. The assay sensitivity was 4.39 pg/mL, and the intra- and inter-assay coefficient variations (CV) were <3% and <6%, respectively

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0054
Volume/Issue:
Volume 11: Issue 7
Open access
Zhengrong Jiang Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Linghong Huang Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Lijun Chen Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Jingxiong Zhou Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Bo Liang Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Xuefeng Bai Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Lizhen Wu Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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Huibin Huang Department of Endocrinology, The Second affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China

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-associated protein proliferating cell nuclear antigen (PCNA) and the apoptosis-associated proteins BCL-XL and BAX were measured using respective ELISA kits (Meimian, Jianshu, China) according to the manufacturer instructions. Key ceRNA network construction and

Article Type:
Research Article
Collections:
Thyroid
DOI:
https://doi.org/10.1530/EC-22-0030
Volume/Issue:
Volume 11: Issue 11
Open access
R C S van Adrichem
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L J Hofland
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R A Feelders
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M C De Martino
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P M van Koetsveld
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C H J van Eijck Department of Internal Medicine, Department of Surgery, Department of Pathology, Sector of Endocrinology, Erasmus MC, ‘s Gravendijkwal 230, Room D-430, 3015 CE Rotterdam, The Netherlands

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R R de Krijger Department of Internal Medicine, Department of Surgery, Department of Pathology, Sector of Endocrinology, Erasmus MC, ‘s Gravendijkwal 230, Room D-430, 3015 CE Rotterdam, The Netherlands

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D M Sprij-Mooij
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J A M J L Janssen
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W W de Herder
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of diagnosis of the GEP NET (baseline). The sCgA levels were measured using a commercially available ELISA method (CIS Bio International, Gif-sur-Yvette cedex, France; upper limit of normal (ULN) 94 μg/l). ‘Nonelevated’ sCgA was defined as ≤2× the ULN

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-13-0052
Volume/Issue:
Volume 2: Issue 4
Open access
Supitcha Patjamontri Developmental Endocrinology Research Group, University of Glasgow, Royal Hospital for Children, Glasgow, UK
Division of Endocrinology and Metabolism, Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

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Alexander Spiers MRC Centre for Environment and Health, Imperial College London, London, UK
NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK

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Rachel B Smith MRC Centre for Environment and Health, Imperial College London, London, UK
NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK
National Institute for Health Research (NIHR) Health Protection Research Unit in Environmental Exposures and Health, Imperial College London, London, UK
Mohn Centre for Children’s Health and Wellbeing, Imperial College London, London, UK

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Chen Shen MRC Centre for Environment and Health, Imperial College London, London, UK
NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK

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Jo Adaway Department of Clinical Biochemistry, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK

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Brian G Keevil Department of Clinical Biochemistry, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK

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Mireille B Toledano MRC Centre for Environment and Health, Imperial College London, London, UK
NIHR Health Protection Research Unit on Chemical Radiation Threats and Hazards, Imperial College London, London, UK
National Institute for Health Research (NIHR) Health Protection Research Unit in Environmental Exposures and Health, Imperial College London, London, UK
Mohn Centre for Children’s Health and Wellbeing, Imperial College London, London, UK

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S Faisal Ahmed Developmental Endocrinology Research Group, University of Glasgow, Royal Hospital for Children, Glasgow, UK

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sample was analysed at 4 further time points up to a maximum of 608 days for steroids assayed by LC-MS/MS and 664 days for steroids assayed by ELISA immunoassay (Salimetrics Assay, State College, PA, USA). Saliva samples for males were analysed for

Article Type:
Research Article
Collections:
Adrenal
DOI:
https://doi.org/10.1530/EC-23-0084
Volume/Issue:
Volume 12: Issue 12
Open access
Xi Zhang Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Graduate School, Wannan Medical College, Wuhu, Anhui, People’s Republic of China

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Xiurong Shen Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Graduate School, Wannan Medical College, Wuhu, Anhui, People’s Republic of China

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Wan Zhou Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Mengyun Xu Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Yan Xing Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Jianping Weng Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Shandong Ye Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Suowen Xu Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Zhi Zhang Hefei National Laboratory for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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Wei Wang Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China
Institute of Endocrine and Metabolic Diseases, University of Science and Technology of China, Hefei, Anhui, People’s Republic of China

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equation. Serum LCN2 levels were assayed using an ELISA kit (Shanghai Xitang Biotechnology Co. Ltd, Shanghai, China). Procedures were according to the manual instructions by kit provider. Assessment of DPN DPN was diagnosed using TCNS based on

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0290
Volume/Issue:
Volume 10: Issue 11
Open access