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Fernando Aprile-Garcia Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina
Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina

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María Antunica-Noguerol Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina
Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina

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Maia Ludmila Budziñski Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina

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Ana C Liberman Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina

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Eduardo Arzt Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina
Instituto de Investigación en Biomedicina de Buenos Aires – CONICET, Departamento de Fisiología, Partner Institute of the Max Planck Society, Buenos Aires, Argentina

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adaptive responses to stressful circumstances. GCs belong to the steroid hormone family, a group of small lipophilic compounds derived from cholesterol, its common precursor. Steroid hormones are generally grouped according to the receptors they bind and

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B Fabre Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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G Maccallini Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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A Oneto Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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D Gonzalez Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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V Hirschler Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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C Aranda Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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G Berg Clinical Biochemistry Department, Hospital Carlos G. Durand Laboratory, TCba Salguero Laboratory, Hospital Carlos G. Durand Nutrition Unit, INFIBIOC, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

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Reviews in Molecular, Cellular and Developmental Biology 2009 31 843 – 852 . 10 Quissell DO . Steroid hormone analysis in human saliva . Annals of the New York Academy of Sciences 1993 694 143 – 145 . ( doi:10.1111/j.1749-6632.1993.tb18348

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Bruno Donadille Service d’Endocrinologie et Médecine de la Reproduction, Centre de Référence des Maladies Endocrines Rares de la Croissance, Hôpital Saint Antoine, Groupe Hospitalier Universitaire Est, AP-HP, Paris, France

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Muriel Houang Service d’Explorations Fonctionnelles Endocriniennes, Centre de Référence des Maladies Endocrines Rares de la Croissance, Hôpital Trousseau, Groupe Hospitalier Universitaire Est, AP-HP, Paris, France

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Irène Netchine Service d’Explorations Fonctionnelles Endocriniennes, Centre de Référence des Maladies Endocrines Rares de la Croissance, Hôpital Trousseau, Groupe Hospitalier Universitaire Est, AP-HP, Paris, France
Université Pierre et Marie Curie, Sorbonne Université, Paris, France

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Jean-Pierre Siffroi Université Pierre et Marie Curie, Sorbonne Université, Paris, France
INSERM UMR_S933, Paris, France

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Sophie Christin-Maitre Service d’Endocrinologie et Médecine de la Reproduction, Centre de Référence des Maladies Endocrines Rares de la Croissance, Hôpital Saint Antoine, Groupe Hospitalier Universitaire Est, AP-HP, Paris, France
Université Pierre et Marie Curie, Sorbonne Université, Paris, France
INSERM UMR_S933, Paris, France

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evaluations have been performed using liquid chromatography-mass spectrometry (LC–MS/MS), which allows steroid hormone analysis of sixteen analytes from a single sample in 150 µL of serum, as previously described ( 6 , 7 , 9 ). Semen was obtained after 4

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Anette Lundqvist Department of Public Health and Clinical Medicine, Family Medicine, Umeå University, Umeå, Sweden

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Herbert Sandström Department of Public Health and Clinical Medicine, Family Medicine, Umeå University, Umeå, Sweden

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Torbjörn Bäckström Department of Clinical Sciences, Obstetrics and Gynecology, Umeå University, Umeå, Sweden

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energy homeostasis to modulate appetite in response to signals by blood-borne hormones and peptides from adipose tissue and gastrointestinal organs in a complicated process ( 13 , 40 ). Allopregnanolone is not a classical steroid hormone and does not

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M Boering Isala, Diabetes Centre, Zwolle, The Netherlands

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P R van Dijk Isala, Diabetes Centre, Zwolle, The Netherlands
Isala, Department of Internal Medicine, Zwolle, The Netherlands

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S J J Logtenberg Diakonessenhuis, Department of Internal Medicine, Utrecht, The Netherlands
Langerhans Medical Research group, Zwolle, The Netherlands

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K H Groenier Isala, Diabetes Centre, Zwolle, The Netherlands
Department of General Practice, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

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B H R Wolffenbuttel Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

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R O B Gans Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

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N Kleefstra Isala, Diabetes Centre, Zwolle, The Netherlands
Langerhans Medical Research group, Zwolle, The Netherlands
Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

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H J G Bilo Isala, Diabetes Centre, Zwolle, The Netherlands
Isala, Department of Internal Medicine, Zwolle, The Netherlands
Department of Internal Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

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-binding globulin mediates steroid hormone signal transduction at the plasma membrane . Journal of Steroid Biochemistry and Molecular Biology 1999 69 481 – 485 . ( doi:10.1016/S0960-0760(99)00070-9 ) 3 Ng Tang Fui M Hoermann R Cheung AS Gianatti EJ

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Piera Rizzolo Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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Valentina Silvestri Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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Virginia Valentini Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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Veronica Zelli Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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Agostino Bucalo Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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Ines Zanna Cancer Risk Factors and Lifestyle Epidemiology Unit, Institute for Cancer Research, Prevention and Clinical Network (ISPRO), Florence, Italy

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Simonetta Bianchi Division of Pathological Anatomy, Department of Sciences of Health, University of Florence, Florence, Italy

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Maria Grazia Tibiletti Department of Pathology, ASST Settelaghi and Centro di Ricerca per lo Studio dei Tumori Eredo-Familiari, Università dell’Insubria, Varese, Italy

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Antonio Russo Section of Medical Oncology, Department of Surgical and Oncological and Oral Sciences, University of Palermo, Palermo, Italy

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Liliana Varesco IRCCS Ospedale Policlinico San Martino, Genoa, Italy

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Gianluca Tedaldi Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy

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Bernardo Bonanni Division of Cancer Prevention and Genetics IEO, European Institute of Oncology IRCCS, Milan, Italy

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Jacopo Azzollini Unit of Medical Genetics, Department of Medical Oncology and Hematology, Fondazione IRCCS Istituto Nazionale dei Tumori (INT), Milan, Italy

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Siranoush Manoukian Unit of Medical Genetics, Department of Medical Oncology and Hematology, Fondazione IRCCS Istituto Nazionale dei Tumori (INT), Milan, Italy

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Anna Coppa Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy

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Giuseppe Giannini Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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Laura Cortesi Department of Oncology and Haematology, University of Modena and Reggio Emilia, Modena, Italy

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Alessandra Viel Unit of Functional Onco-Genomics and Genetics, Centro di Riferimento Oncologico di Aviano (CRO), IRCCS, Aviano, Italy

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Marco Montagna Immunology and Molecular Oncology Unit, Veneto Institute of Oncology IOV – IRCCS, Padua, Italy

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Paolo Peterlongo Genome Diagnostics Program, IFOM – The FIRC Institute of Molecular Oncology, Milan, Italy

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Paolo Radice Unit of Molecular Bases of Genetic Risk and Genetic Testing, Department of Research, Fondazione IRCCS Istituto Nazionale Tumori (INT), Milan, Italy

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Domenico Palli Cancer Risk Factors and Lifestyle Epidemiology Unit, Institute for Cancer Research, Prevention and Clinical Network (ISPRO), Florence, Italy

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Laura Ottini Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy

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, such as BC, by altering the expression of steroid hormones, including estrogens ( 9 , 10 , 11 ). CYP17A1 is an enzyme essential for the biosynthesis of estrogens and androgens ( 12 ). A common polymorphism in the promoter of CYP17A1 (c.-34T>C; rs

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Lauren R Cirrincione Department of Pharmacy, University of Washington, Seattle, Washington, USA

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Bridgit O Crews Department of Pathology and Laboratory Medicine, University of California Irvine, Orange, California, USA

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Jane A Dickerson Department of Laboratories, Seattle Children's Hospital, Seattle, Washington, USA

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Matthew D Krasowski Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, Iowa, USA

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Jessica Rongitsch Internal Medicine, Capitol Hill Medical, Seattle, Washington, USA

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Katherine L Imborek Department of Family Medicine, University of Iowa Hospitals and Clinics, Iowa City, Iowa, USA

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Zil Goldstein Department of Medicine, Callen-Lorde Community Health Center, New York, New York, USA
CUNY Graduate School of Public Health and Health Policy, New York, New York, USA

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Dina N Greene Washington Kaiser Permanente, Renton, Washington, USA
Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA

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estrone in feminizing hormone treatment . Journal of Clinical Endocrinology and Metabolism 2021 107 e458 – e466 . ( https://doi.org/10.1210/clinem/dgab741 ) 23 Stanczyk FZ Lee JS Santen RJ . Standardization of steroid hormone assays: why, how

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Alessandra Gambineri Endocrinology Unit, Department of Medical and Surgical Sciences, St Orsola-Malpighi Hospital, Alma Mater University of Bologna, Bologna, Italy

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Carla Pelusi Endocrinology Unit, Department of Medical and Surgical Sciences, St Orsola-Malpighi Hospital, Alma Mater University of Bologna, Bologna, Italy

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tissues, particularly on adipose tissue. The sexual dimorphism of androgens in the pathophysiology of T2DM is emphasized. Sex hormone differences between the sexes Males and females produce the same steroid hormones in a similar way. Regarding

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Lesley A Hill Departments of Cellular and Physiological Sciences and Obstetrics and Gynaecology, The University of British Columbia, Vancouver, British Columbia, Canada

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Zeynep Sumer-Bayraktar School of Life and Environmental Science, Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia

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John G Lewis Canterbury Health Laboratories, Christchurch, New Zealand

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Eva Morava Mayo Clinic, Department of Clinical Genomics, CIM, Rochester, Minnesota, USA

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Morten Thaysen-Andersen Department of Molecular Sciences, Macquarie University, Sydney, New South Wales, Australia

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Geoffrey L Hammond Departments of Cellular and Physiological Sciences and Obstetrics and Gynaecology, The University of British Columbia, Vancouver, British Columbia, Canada

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manuscript for publication. Acknowledgement The authors wish to thank Caroline Underhill for technical assistance. References 1 Hammond GL . Plasma steroid-binding proteins: primary gatekeepers of steroid hormone action . Journal of

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Stine A Holmboe Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

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Ravi Jasuja Research Program in Men’s Health: Aging and Metabolism, Brigham and Womens Hospital, Harvard Medical School, Boston, Massachusetts, USA

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Brian Lawney Research Program in Men’s Health: Aging and Metabolism, Brigham and Womens Hospital, Harvard Medical School, Boston, Massachusetts, USA

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Lærke Priskorn Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

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Niels Joergensen Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

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Allan Linneberg Centre for Clinical Research and Prevention, Frederiksberg Hospital, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Tina Kold Jensen Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Department of Environmental Medicine, Institute of Public Health, University of Southern Denmark, Odense, Denmark

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Niels Erik Skakkebæk Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

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Anders Juul Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

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Anna-Maria Andersson Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
The International Research Centre in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

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reliable compared to methods based on liquid or gas chromatography tandem mass spectrometry, which today are recommended for analyses of small molecules like steroid hormones ( 1 , 33 ). The main problem of immunoassays relates to their tendency of

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