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, and demonstrated a strong positive correlation between mRNA levels for HNF4α and SHBG, and found an inverse relationship with the amount of liver triglyceride ( 19 ). It is now known that HNF4α expression is reduced in rodents fed a high fat diet ( 20
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Aix-Marseille Université, Faculté de Médecine, Marseille, France
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loop. Binding of miRNA to target messenger RNA (mRNA) leads to translational suppression or mRNA degradation ( 17 ). Partial complementarity is often sufficient for binding ( 16 ), meaning that individual miRNAs may have hundreds of different mRNA
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towards an increased concentration of thyroid hormone metabolites. Placental expression of the isoforms THRα1, THRβ1 and THRα2 increases with gestational age ( 14 ). In a previous study, we could demonstrate that mRNA and protein expression of THRα1, THRα2
Université Côte d'Azur, INSERM, C3M, Team Cellular and Molecular Physiopathology of Obesity and Diabetes, Nice, France
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mediator linking obesity with BC. Accumulated evidence supports this hypothesis: (1.) Hyperleptinemia and intratumoral leptin mRNA levels have been linked to a poor prognosis in BC patients ( 4 ), (2.) the leptin receptor (ObR) is highly expressed in
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liver biopsies ( Table 1 ). Our data showed strong expression of both TH receptors, SLC16A2 , SLC10A1 and DIO1 , whereas the mRNA levels of SLCO1C1 , DIO2 and DIO3 were more than 10-fold lower, suggesting negligible biological relevance ( Fig. 1
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Medical Microbiology Department, College of Basic Medicine, Qingdao University, Qingdao, China
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Physiology Department, College of Basic Medicine, Qingdao University, Qingdao, China
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) could induce polyphagia and body weight gain ( 13 ). These effects were also observed following ghrelin injection into the fourth ventricle and dorsal vagal complex (DVC) in brainstem ( 14 ). Additionally, GHSR mRNA has been detected in the lateral
The First Affiliated Hospital, Department of Otorhinolaryngology, Hengyang Medical School, University of South China, Hengyang, Hunan, China
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The First Affiliated Hospital, Department of Endocrinology and Metabolism, Hengyang Medical School, University of South China, Hengyang, Hunan, China
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Bonferroni’s post hoc test. The difference between the four groups in the rescue experiments was analyzed by two-way ANOVA. Spearman correlation analysis was used to assess the correlation between FGF1 and HMGA1 mRNA levels in PTC tissues. P values < 0
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concentration and purity of RNA were detected, and the extracted RNA was reverse transcribed into cDNA by Takara reverse transcription kit (RR037A, Takara Bio). Then, the mRNA expression was quantitatively detected using the Takara quantitative kit (RR014A
Laboratory Medicine, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands
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. Data analysis Gene expression mRNA expression of all genes was calculated using the delta Ct method (2 −∆Ct ). All values were normalized to the corresponding HPRT value ( 29 ). Data were transferred to GraphPad Prism 5 and IBM SPSS 22.0 (SPSS
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Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia
Concord Clinical School, The University of Sydney, Sydney, Australia
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Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University, Shaanxi, China
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Concord Clinical School, The University of Sydney, Sydney, Australia
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commercial reverse transcription kit (SuperScript III First-Strand Synthesis for qRT-PCR, Life Technologies). Complementary DNA was synthesized from 600 µg RNA, and 200 µL Milli Q water was added to each cDNA sample for dilution before qPCR. Relative mRNA